Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (1): 13-20.doi: 10.6040/j.issn.1671-7554.0.2021.1002

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Repairing effect of a self-constructed chitosan nerve conduit on facial nerve injury in rats

XU Feifei1,2, CHEN Qingwen1,2, WANG Dongqin1,2, DING Bixiao1,2, WU Hao1, YANG Yumin3   

  1. 1. Department of Otolaryngology Head Neck Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, China;
    2. Medical School of Nantong University, Nantong 226001, Jiangsu, China;
    3. Key Laboratory of Neural Regeneration, Nantong University, Nantong 226001, Jiangsu, China
  • Published:2022-01-08

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Abstract: Objective The facial nerve defect of model rats was repaired with a chitosan artificial nerve conduit independently constructed by the Key Laboratory of Nerve Regeneration of Nantong University in order to evaluate its role in facial nerve function recovery and regeneration. Methods A total of 24 rats were divided into 3 groups, with 8 in each group. The defect models of superior buccal branch of right facial nerve were established and treated with autogenous nerve overturning bridging(autogenous nerve group), chitosan nerve conduit bridging(chitosan group)and amputation(amputation group)respectively. The recovery of innervation function of facial nerve was evaluated with whisker motor score at 2, 4, 6, 8, 10 and 12 weeks after treatment. The diameter, thickness and number of layers of myelin sheath and axon of contralateral normal nerve and the facial nerve 12 weeks after treatment were observed with immunohistochemical double staining and transmission electron microscope. Results After the analysis of tentacle motor score with generalized linear mixed effect model(GLMMs), the main effects of grouping and time were statistically significant(P<0.05), and the interaction between grouping and time was statistically significant(P<0.05). The recovery of tentacle motor function began to appear in autologous nerve group and chitosan group at 4 and 6 weeks after treatment, respectively. There was no significant difference between autologous nerve group and chitosan group 12 weeks after operation(P>0.05). There was no recovery of tentacle motor function in amputation group. Morphological observation and transmission electron microscope showed that regenerated nerves were found in autologous nerve group and chitosan group. There were significant differences in the diameter of myelinated nerve fibers(F=36.734, P<0.05), sheath thickness(F=67.307, P<0.05)and the number of regenerated myelin sheath layers(F=75.213, P<0.05)in autologous nerve group, chitosan group and the contralateral normal nerves. The diameter of the myelinated nerve fiber, sheath thickness and the number of regenerated myelin sheath layers in chitosan group were significantly different from those in autologous nerve group(P<0.05). Conclusion The chitosan artificial nerve conduit can promote the regeneration and functional recovery of facial nerve injury, which provides a basis for clinical repair of peripheral nerve besides sciatic nerve and radial nerve.

Key words: Chitosan, Facial nerve regeneration, Injury, Repair

CLC Number: 

  • R651.3
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