Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (6): 1-5.doi: 10.6040/j.issn.1671-7554.0.2018.109

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Application of Suiplus semen analysis automatic detection system and manual counting using hemocytometer in mouse semen analysis

SU Xiaohui1, SUN Mingqi1, ZHANG Meijie1, FANG Li1, LUO Dandan1, CHEN Guoping2, YU Chunxiao1, GUAN Qingbo1   

  1. 1. Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University;
    Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism;
    Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan 250021, Shandong, China;
    2. Beijing Suiplus Software Co. Ltd, Beijing 100086, China
  • Published:2022-09-27

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Abstract: Objective To compare the functions and differences of Suiplus semen analysis(SSA)automatic detection system and manual counting using hemocytometer in mouse semen analysis. Methods The general morphology, concentration, kinematic parameters and motility of sperm samples of adult male C57BL/6 mice were measured by the above two methods respectively. The data were statistically analyzed. Results The sperm concentration detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). The results of the SSA system showed that the straight line velocity was(25.41±6.42)μm/s, the elocity in curvilinear velocity was(74.13±5.82)μm/s, the linearity was(24.63±8.43)%, and the straightness was(41.00±9.62)%. The proportion of motile sperm detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). Conclusion SSA automatic 山 东 大 学 学 报 (医 学 版)56卷6期 -苏晓慧,等.穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用 \=-detection system can directly observe the morphology and movement of sperm, and analyze the corresponding parameters simply but accurately, which provides a convenient and reliable method for semen analysis.

Key words: Semen analysis, Suiplus semen analysis automatic detection system, Manual counting using hemocytometer, Mouse

CLC Number: 

  • R588.1
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