Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (6): 1-5.doi: 10.6040/j.issn.1671-7554.0.2018.109

    Next Articles

Application of Suiplus semen analysis automatic detection system and manual counting using hemocytometer in mouse semen analysis

SU Xiaohui1, SUN Mingqi1, ZHANG Meijie1, FANG Li1, LUO Dandan1, CHEN Guoping2, YU Chunxiao1, GUAN Qingbo1   

  1. 1. Department of Endocrinology, Shandong Provincial Hospital Affiliated to Shandong University;
    Shandong Provincial Key Laboratory of Endocrinology and Lipid Metabolism;
    Institute of Endocrinology and Metabolism, Shandong Academy of Clinical Medicine, Jinan 250021, Shandong, China;
    2. Beijing Suiplus Software Co. Ltd, Beijing 100086, China
  • Published:2022-09-27

PDF (PC)

109

Abstract: Objective To compare the functions and differences of Suiplus semen analysis(SSA)automatic detection system and manual counting using hemocytometer in mouse semen analysis. Methods The general morphology, concentration, kinematic parameters and motility of sperm samples of adult male C57BL/6 mice were measured by the above two methods respectively. The data were statistically analyzed. Results The sperm concentration detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). The results of the SSA system showed that the straight line velocity was(25.41±6.42)μm/s, the elocity in curvilinear velocity was(74.13±5.82)μm/s, the linearity was(24.63±8.43)%, and the straightness was(41.00±9.62)%. The proportion of motile sperm detected by the SSA system was significantly higher than that detected by manual counting(P<0.05). Conclusion SSA automatic 山 东 大 学 学 报 (医 学 版)56卷6期 -苏晓慧,等.穗加精液分析自动检测系统与血细胞计数板人工计数法在小鼠精液分析中的应用 \=-detection system can directly observe the morphology and movement of sperm, and analyze the corresponding parameters simply but accurately, which provides a convenient and reliable method for semen analysis.

Key words: Semen analysis, Suiplus semen analysis automatic detection system, Manual counting using hemocytometer, Mouse

CLC Number: 

  • R588.1
[1] Skakkebaek NE, Rajpert-De Meyts E, Buck Louis GM, et al. Male reproductive disorders and fertility trends: influences of environment and genetic susceptibility[J]. Physiol Rev, 2016, 96(1): 55-97.
[2] Adewoyin M, Ibrahim M, Roszaman R, et al. Male infertility: the effect of natural antioxidants and phytocompounds on seminal oxidative stress[J]. Diseases, 2017, 5(1): 9. doi: 10.3390/diseases5010009
[3] 吴园园, 韩玉芳, 王瑞红, 等. 计算机辅助精液分析与手工计数法检测精液的比较研究[J]. 国际检验医学杂志, 2017, 38(12): 1726-1727. doi:10.3969/j.issn.1673-4130.2017.12.061
[4] PENG Nanni, ZOU Xiangli, LI Lingwei. Comparison of different counting chambers using a computer-assisted semen analyzer[J]. Syst Biol Reprod Med, 2015, 61(5): 307-313.
[5] 申涌, 刘咏梅. 枸杞多糖对甲醛所致雄性小鼠生殖毒性的拮抗作用[J]. 贵州医科大学学报, 2017, 42(4): 440-443. SHEN Yong, LIU Yongmei. Study on the antagonism of lycium barbarum polysaccharides on formaldehyde induced male mice reproductive toxicity[J]. Journal of Guizhou Medical University, 2017, 42(4): 440-443.
[6] Kumari K, Capstick M, Cassara AM, et al. Effects of intermediate frequency magnetic fields on male fertility indicators in mice[J]. Environ Res, 2017, 157: 64-70. doi:10.1016/j.envres.2017.05.014
[7] 李文娟, 王晋. 间歇低氧对小鼠精子生成数量及活力的影响[J]. 中国继续医学教育, 2015, 7(18): 82-83. LI Wenjuan, WANG Jin. The effects of intermittent hypoxia on sperm number and activity in mice[J]. China Continuing Medical Education, 2015, 7(18): 82-83.
[8] Miller MR, Mansell SA, Meyers SA, et al. Flagellar ion channels of sperm: similarities and differences between species[J]. Cell Calcium, 2015, 58(1): 105-113.
[9] 世界卫生组织. 人类精液检查及处理实验室手册[M]. 5版. 北京: 人民卫生出版社, 2011: 117-118.
[10] Nikmehr B, Bazrafkan M, Hassanzadeh G, et al. The correlation of gene expression of inflammasome indicators and impaired fertility in rat model of spinal cord injury: a time course study[J]. Urol J, 2017, 14(6): 5057-5063.
[11] 史晓蓉, 刘舒瑜, 陈亚, 等. 芹菜素对小鼠精液参数影响的研究[J]. 中华男科学杂志, 2010, 16(9): 778-782. SHI Xiaorong, LIU Shuyu, CHEN Ya, et al. Apigenin affects semen parameters in male mice[J]. National Journal of Andrology, 2010, 16(9): 778-782.
[12] Nelson VL, Negrón AL, Reid I, et al. Loss of PI3K p110α in the adipose tissue results in infertility and delayed puberty onset in male mice[J]. Biomed Res Int, 2017, 2017: 3756089. doi:10.1155/2017/3756089
[13] ZANG Zhijun, FANG Youqiang, JI Suyun, et al. Formaldehyde inhibits sexual behavior and expression of steroidogenic enzymes in the testes of mice[J]. J Sex Med, 2017, 14(11): 1297-1306.
[14] Moretti E, Collodel G, Mazzi L, et al. Ultrastructural study of spermatogenesis in KSR2 deficient mice[J]. Transgenic Res, 2015, 24(4): 741-751.
[15] Dardmeh F, Alipour H, Gazerani P, et al. Lactobacillus rhamnosus PB01(DSM 14870)supplementation affects markers of sperm kinematic parameters in a diet-induced obesity mice model[J]. PLoS One, 2017, 12(10): e0185964. doi:10.1371/journal.pone.0185964.
[16] Weng Z, Ohtani K, Suda M, et al. Assessment of the reproductive toxicity of inhalation exposure to ethyl tertiary butyl ether in male mice with normal, low active and inactive ALDH2[J]. Arch Toxicol, 2014, 88(4): 1007-1021.
[17] WANG Huafeng, CHANG Meng, PENG Tingting, et al. Exposure to cadmium impairs sperm functions by reducing CatSper in mice[J]. Cell Physiol Biochem, 2017, 42(1): 44-54.
[18] 陆金春, 岳茹倩, 冯瑞祥, 等. 精子计数池深度对精子活力影响的实验研究[J]. 中华男科学杂志, 2013, 19(9): 776-779. LU Jinchun, YUE Ruqian, FENG Ruixiang, et al. Influence of the depth of the sperm counting chamber on sperm motility[J]. National Journal of Andrology, 2013, 19(9): 776-779.
[19] Brito LF, Althouse GC, Aurich C, et al. Andrology laboratory review: evaluation of sperm concentration[J]. Theriogenology, 2016, 85(9): 1507-1527.
[20] LU Jinchun, YUE Ruqian, FENG Ruixiang, et al. Accuracy evaluation of the depth of six kinds of sperm counting chambers for both manual and computer-aided semen analyses[J]. Int J Fertil Steril, 2016, 9(4): 527-533.
[21] Auger J, Jouannet P, Eustache F. Another look at human sperm morphology[J]. Hum Reprod, 2016, 31(1): 10-23.
[22] 王丽琼, 陈建伟, 齐德生, 等. 精子DNA单双链损伤与精子浓度的关系研究[J]. 中国性科学, 2017, 26(3): 87-90. WANG Liqiong, CHEN Jianwei, QI Desheng, et al. Study on the relationship between sperm nuclear DNA damage and sperm production[J]. The Chinese Journal of Human Sexuality, 2017, 26(3): 87-90.
[23] 刘政, 刘子巍, 林冲, 等. 亚慢性低浓度苯暴露对小鼠精子活力的影响[J]. 毒理学杂志, 2013, 27(3): 207-209.
[24] Young SA, Miyata H, Satouh Y, et al. CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa[J]. J Cell Sci, 2016, 129(23): 4379-4387.
[25] Miura N, Ohtani K, Hasegawa T, et al. High sensitivity of testicular function to titanium nanoparticles[J]. J Toxicol Sci, 2017, 42(3): 359-366.
[26] 张永涛. 男性不育患者精液常规检查结果中的异常项目分析[J]. 河南医学研究, 2017, 26(15): 2715-2716.
[1] YANG Xiao-Qian, JI Jing, LIU Na, GUO Dong-Mei, CUI Xi. Effect of FeCl3 and FeEDTA on inhibiting psoriasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 114-117.
[2] LIU Min, ZHANG Yuchao, MA Xiaoli, LIU Xinyu, SUN Lu, ZUO Dan, LIU Yuantao. Effects of orphan nuclear receptor NR4A1 on H2O2 induced injury of mouse renal podocytes [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 16-21.
[3] LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde. Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 15-23.
[4] SUO Dongyang, SHEN Fei, GUO Hao, LIU Lichang, YANG Huimin, YANG Xiangdong. Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 1-6.
[5] ZHU Lin, LI Weiwei, LIU Zhikai. Human stromal vascular fraction combined with adipose stem cells promotes the healing of radiation skin damage in nude mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 66-72.
[6] LI Hongzhi, SHEN Yongchao, LIU Jieting, ZHAO Xiaojin, CHU Yanhui, YUAN Xiaohuan. An 11β-HSD1 inhibitor improves insulin sensitivity in db/db mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 59-64.
[7] SHI Rui, SUN Pei, WANG Lulu, DING Lin, XIA Jin, WANG Yan, PANG Shuguang. Vitamin D and mecobalamin combined with mouse nerve growth factor for diabetic peripheral neuropathy: a clinical observation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 64-67.
[8] JIA Junwei, ZHOU Xueying, LI Qiuhong, YU Kai, ZHOU Guoyu, ZHOU Shengnian. Expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 6-10.
[9] ZHAI Hongyun, ZHANG Denglu, WANG Guangjie, KONG Feng, CHENG Guanghui, ZHAO Shengtian. Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 8-12.
[10] XIAO Fang, JIANG Weidong, WANG Xiaohong, QIN Aiqiong, HAN Min, CHEN Li. Extraction and identification of mouse islet microvascular endothelial cells (MS-1)-derived exosomes [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 6-9.
[11] ZHANG Zhongxia, MA Xiaowei, WANG Yanyong, LI Xiaoli, WANG Mingwei. Impact of aging on the nigro-strital oxidative stress in a mice model of Parkinson's disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 26-29.
[12] WEN Yan1, ZHAO Shuqin2, SHEN Yanjun1, CHAO Lan1, SONG Changzheng3, DENG Xiaohui1. Exploration of the protective role of antifreeze protein III on mouse embryo in vitrification [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 35-38.
[13] WANG Hongyan, WANG Yanrong, XU Xian, ZHAO Junli, HA Lingxia, JIA Shaotong, LIU Chunlian. Fertility restore of allotransplantation of vitrified-warmed junvenile ovaries treated with a modified vitrification solution [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 27-32.
[14] SONG Ling-gang1, LI Xiao-dong2, MA Zhi-yong1, WANG Yuan-yuan1, WANG Jian-chun2, LI Li1. Effect of  the β1-adrenoceptor antibody on potassium channels in  mouse cardiac myocytes [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 58-.
[15] TIAN Bao-guo1, WANG Ping1, NIU Jian-yi2, WANG Zi-ping2, ZHAO Hai-qing2, BI Jian-zhong1. G-CSF and VEGF increase expressions of CD133 and synaptophysin in  the APP transgenic mouse model [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 39-42.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd