JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (10): 6-10.doi: 10.6040/j.issn.1671-7554.0.2015.064

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The inhibitory effect of nitidine chloride on pituitary adenoma GH3 cells

LI Tao, WU Hongxi, ZHANG Yongchao, ZHENG Zhiming, ZHANG Zhen, TENG Liangzhu   

  1. Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2015-01-16 Online:2015-10-10 Published:2015-10-10

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Abstract: Objective To explore the inhibitory effect and role of nitidine chloride on pituitary adenoma GH3 cells. Methods After GH3 cells were treated with different concentrations of nitidine chloride for different periods, cell viability was detected using Cell Counting Kit 8 (CCK8) assay, apoptosis was analyzed using Annexin V/PI Apoptosis Detection kit by flow cytometry, and cell cycle distribution was evaluated using PI staining by flow cytometry. The mRNA expression levels of apoptosis related moleculars Bax and Bcl-2 and cell cycle related moleculars Cyclin B1, CDK1, p21 and p27 were detected with real time PCR. Protein expression levels of Bax, Bcl-2, Cyclin B1, CDK1, activation of AKT signal and ERK signal were determined with Western blotting. Results Nitidine chloride inhibited GH3 cell proliferation, induced apoptosis, arrested cells in G2/M stage, and blocked the activation of AKT and ERK signaling pathway. Conclusion Nitidine chloride inhibits cell proliferation and induces apoptosis of pituitary adenoma GH3 cells. It could be an effective therapeutic agent against pituitary adenoma.

Key words: Nitidine Chloride, Apoptosis, AKT, ERK, Cell cycle, Pituitary adenoma

CLC Number: 

  • R736.4
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