JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 16-20.doi: 10.6040/j.issn.1671-7554.0.2014.359

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Impact of miR-486-5p on the biological behavior of colorectal carcinoma SW620 cells

LIU Chengxia1, LI Ming2, LIAN Haifeng1, SHI Ning1   

  1. 1. Department of Gastroenterology, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong, China;
    2. Department of Gastroenterology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong, China
  • Received:2014-06-03 Revised:2014-12-17 Published:2015-01-10

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Abstract: Objective To investigate the effect of microRNA-486-5p (miR-486-5p) on the proliferation, apoptosis and migration of human colorectal cancer cell line SW620. Methods The miR-486-5p over-expressing plasmid was constructed and transfected into human colorectal carcinoma cell line SW620 using LipofectamineTM2000. The expression of miR-486-5p of the transfected cells was measured with quantitative real-time PCR (qRT-PCR), the proliferation and apoptosis of SW620 cells were assessed with MTT and flow cytometry, and the in vitro migration abilities of SW620 cells was detected with Transwell test. Results The expression of miR-486-5p in SW620 cells was markedly up-regulated (8.72±0.52 vs 1.02±0.06). The proliferation (0.92±0.02 vs 1.40±0.03) and migration abilities of SW620 cells (75.20±10.60 vs 142.50±11.30)were inhibited significantly, and apoptosis rate[(15.30%±2.60)% vs (4.70±1.30)%] was increased (P<0.05). Conclusion MiR-486-5p can effectively suppress the proliferation and in vitro migration abilities of SW620 cells, and increase the apoptosis rate, indicating that miR-486-5p might be used as a target for molecular therapy of colorectal cancer.

Key words: Colorectal carcinoma, Apoptosis, MiR-486-5p, Transfection, Proliferation, Migration

CLC Number: 

  • R574
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