睾酮对于多囊卵巢综合征易感基因Tox3表达的影响

doi:10.6040/j.issn.1671-7554.0.2021.0056

摘要/Abstract

摘要： 目的确定多囊卵巢综合征(PCOS)易感基因Tox3在野生型斑马鱼模型中的表达模式,探讨睾酮对于Tox3基因表达的影响。方法取斑马鱼胚胎约200枚与雌雄成年斑马鱼各约100条进行对照处理及睾酮(10 ng/mL)处理,利用反转录-聚合酶链反应(RT-PCR)及实时荧光定量逆转录聚合酶链反应(qRT-PCR)技术,检测各组斑马鱼中Tox3基因的表达特性。采用胚胎整体原位杂交(WISH)方法,在数枚斑马鱼胚胎内定位观察Tox3基因的表达以及分布。结果野生型斑马鱼的神经系统和生殖系统中Tox3基因表达水平较高。与对照组相比,雄性斑马鱼Tox3的表达在10 ng/mL睾酮处理后的精巢［(1.05±0.32) vs(1.50±0.30), P=0.015］及脑［(1.01±0.19)vs(1.43±0.35), P=0.043］中均升高。结论斑马鱼Tox3基因可能通过作用于神经内分泌系统,参与雄激素合成。

关键词: 睾酮, 多囊卵巢综合征, Tox3, 基因表达, 斑马鱼

Abstract: Objective To determine the expression of polycystic ovary syndrome(PCOS)susceptibility gene Tox3 in wild-type zebrafish, and explore the effect of testosterone on Tox3 gene expression. Methods About 200 zebrafish embryos and 100 male and female adult zebrafish for control treatment and testosterone(10 ng/mL)treatment were required. Reverse transcription-polymerase chain reaction(RT-PCR)and real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)were used to test the expression of Tox3 gene in zebrafish. The distributions of Tox3 mRNA in zebrafish embryos were observed by whole mount in situ hybridization(WISH). Results In wild-type zebrafish, the level of Tox3 gene expression was high in the nervous system and reproductive system. After treated with 10 ng/mL testosterone, compared with the control group, the Tox3 gene expression of male zebrafish treated with 10 ng/mL testosterone was higher in brain ［(1.01±0.19) vs(1.43±0.35),P=0.043］ and in nest ［(1.05±0.32) vs(1.50±0.30),P=0.015］. Conclusion Tox3 in zebrafish may regulates the synthesis and release of androgen by acting on neuroendocrine systems.

Key words: Testosterone, Polycystic ovary syndrome, Tox3, Gene expression, Zebrafish

中图分类号:

R711

韩笑,李雷,刘聪聪. 睾酮对于多囊卵巢综合征易感基因Tox3表达的影响[J]. 山东大学学报 (医学版), 2021, 59(4): 42-47.

HAN Xiao, LI Lei, LIU Congcong. Effect of testosterone on the expression of polycystic ovary syndrome susceptibility gene Tox3 in zebrafish[J]. Journal of Shandong University (Health Sciences), 2021, 59(4): 42-47.

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