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山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (4): 58-63.doi: 10.6040/j.issn.1671-7554.0.2017.998

• 临床医学 • 上一篇    



  1. 山东大学附属生殖医院 国家辅助生殖与优生工程技术研究中心 〓生殖内分泌教育部重点实验室(山东大学)山东省生殖医学重点实验室, 山东 济南 250001
  • 发布日期:2022-09-27
  • 通讯作者: 刘洪彬. E-mail:humangenetics2008@hotmail.com
  • 基金资助:

Expression of FBP1 in granulosa cells of PCOS patients with hyperandrogenism and its significance

SHU Xin, WANG Qun, HUANG Tao, ZHAO Shigang, SHI Yuhua, LIU Hongbin   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China
  • Published:2022-09-27

摘要: 目的 检测多囊卵巢综合征(PCOS)高雄激素血症患者卵巢颗粒细胞中果糖-1,6-二磷酸酶1(FBP1)基因的表达,并评价其临床意义。 方法 收集100例PCOS患者的卵泡液颗粒细胞,其中PCOS高雄激素血症患者(PCOS高雄激素组)28例、PCOS非高雄激素血症患者(PCOS非高雄激素组)72例、同期正常对照患者(正常对照组)58例。采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测各组卵泡液颗粒细胞中FBP1的表达,并对FBP1的表达和高雄激素相关指标进行关联性分析,同时收集患者年龄、体质量指数(BMI)、相关激素等进行回顾分析。 结果 PCOS高雄激素组中超重或肥胖、高血压、高血糖和代谢综合征的发生率均高于PCOS非高雄激素组和正常对照组(P<0.05)。FBP1 mRNA在PCOS高雄激素组患者卵泡液颗粒细胞中的表达高于正常对照组(P=0.002)及PCOS非高雄激素组(P=0.038)。PCOS高雄激素组FBP1 mRNA的相对表达量与硫酸脱氢表雄酮(DHE-S)呈正相关(r=0.470, P=0.021)。 结论 FBP1在PCOS高雄激素血症患者卵泡液颗粒细胞中高表达,并与DHE-S相关,其可能通过卵泡局部微环境参与PCOS高雄激素血症的致病过程。

关键词: 多囊卵巢综合征, FBP1基因, 颗粒细胞, 高雄激素血症, 硫酸脱氢表雄酮

Abstract: Objective To explore the expression and significance of FBP1 in ovarian granulosa cells in patients with polycystic ovary syndrome(PCOS). Methods A total of 28 PCOS patients with hyperandrogenism(hyperandrogenism group), 72 PCOS patients without hyperandrogenism(non-hyperandrogenism group)and 58 healthy controls(control group)were enrolled. The expressions of FBP1 in the ovarian granulosa cells of the three groups were examined with real-time quantitative reverse transcription PCR(qRT-PCR). The correlation between FBP1 and androgen-related indicators was analyzed. The patients age, body mass index(BMI)and related hormones were analyzed. Results The hyperandrogenism group had higher incidence of obesity, hypertensive disease, hyperglycemia, metabolic syndrome and morbidity than the non-hyperandrogenism and control groups(P<0.05). The mRNA expression of FBP1 was higher in the hyperandrogenism group than that in the non-hyperandrogenism group(P=0.038)and control group(P=0.002). There was positive correlation between dehydroepiandrosterone sulfate(DHE-S)and the mRNA expression of 山 东 大 学 学 报 (医 学 版)56卷4期 -舒心,等.多囊卵巢综合征高雄激素血症患者颗粒细胞FBP1基因的表达及影响 \=-FBP1 in the hyperandrogenism group(r=0.470, P=0.021). Conclusion High expression of FBP1 is associated with hyperandrogenism of PCOS and DHE-S, and may be involved in the pathogenesis of PCOS hyperandrogenism through follicular microenvironment.

Key words: Polycystic ovary syndrome, FBP1 gene, Granulosa cell, Hyperandrogenism, Dehydroepiandrosterone sulfate


  • R711.75
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