多囊卵巢综合征高雄激素血症患者颗粒细胞FBP1基因的表达及影响

doi:10.6040/j.issn.1671-7554.0.2017.998

摘要/Abstract

摘要： 目的检测多囊卵巢综合征(PCOS)高雄激素血症患者卵巢颗粒细胞中果糖-1,6-二磷酸酶1(FBP1)基因的表达,并评价其临床意义。方法收集100例PCOS患者的卵泡液颗粒细胞,其中PCOS高雄激素血症患者(PCOS高雄激素组)28例、PCOS非高雄激素血症患者(PCOS非高雄激素组)72例、同期正常对照患者(正常对照组)58例。采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测各组卵泡液颗粒细胞中FBP1的表达,并对FBP1的表达和高雄激素相关指标进行关联性分析,同时收集患者年龄、体质量指数(BMI)、相关激素等进行回顾分析。结果 PCOS高雄激素组中超重或肥胖、高血压、高血糖和代谢综合征的发生率均高于PCOS非高雄激素组和正常对照组(P<0.05)。FBP1 mRNA在PCOS高雄激素组患者卵泡液颗粒细胞中的表达高于正常对照组(P=0.002)及PCOS非高雄激素组(P=0.038)。PCOS高雄激素组FBP1 mRNA的相对表达量与硫酸脱氢表雄酮(DHE-S)呈正相关(r=0.470, P=0.021)。结论 FBP1在PCOS高雄激素血症患者卵泡液颗粒细胞中高表达,并与DHE-S相关,其可能通过卵泡局部微环境参与PCOS高雄激素血症的致病过程。

关键词: 多囊卵巢综合征, FBP1基因, 颗粒细胞, 高雄激素血症, 硫酸脱氢表雄酮

Abstract: Objective To explore the expression and significance of FBP1 in ovarian granulosa cells in patients with polycystic ovary syndrome(PCOS). Methods A total of 28 PCOS patients with hyperandrogenism(hyperandrogenism group), 72 PCOS patients without hyperandrogenism(non-hyperandrogenism group)and 58 healthy controls(control group)were enrolled. The expressions of FBP1 in the ovarian granulosa cells of the three groups were examined with real-time quantitative reverse transcription PCR(qRT-PCR). The correlation between FBP1 and androgen-related indicators was analyzed. The patients age, body mass index(BMI)and related hormones were analyzed. Results The hyperandrogenism group had higher incidence of obesity, hypertensive disease, hyperglycemia, metabolic syndrome and morbidity than the non-hyperandrogenism and control groups(P<0.05). The mRNA expression of FBP1 was higher in the hyperandrogenism group than that in the non-hyperandrogenism group(P=0.038)and control group(P=0.002). There was positive correlation between dehydroepiandrosterone sulfate(DHE-S)and the mRNA expression of 山东大学学报 (医学版)56卷4期 -舒心,等.多囊卵巢综合征高雄激素血症患者颗粒细胞FBP1基因的表达及影响 \=-FBP1 in the hyperandrogenism group(r=0.470, P=0.021). Conclusion High expression of FBP1 is associated with hyperandrogenism of PCOS and DHE-S, and may be involved in the pathogenesis of PCOS hyperandrogenism through follicular microenvironment.

Key words: Polycystic ovary syndrome, FBP1 gene, Granulosa cell, Hyperandrogenism, Dehydroepiandrosterone sulfate

中图分类号:

R711.75

舒心,王群,黄涛,赵世刚,石玉华,刘洪彬. 多囊卵巢综合征高雄激素血症患者颗粒细胞FBP1基因的表达及影响[J]. 山东大学学报 (医学版), 2018, 56(4): 58-63.

SHU Xin, WANG Qun, HUANG Tao, ZHAO Shigang, SHI Yuhua, LIU Hongbin. Expression of FBP1 in granulosa cells of PCOS patients with hyperandrogenism and its significance[J]. Journal of Shandong University (Health Sciences), 2018, 56(4): 58-63.

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