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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (1): 69-74.doi: 10.6040/j.issn.1671-7554.0.2016.1313

• 临床医学 • 上一篇    下一篇

microRNA-183在PCOS胰岛素抵抗中的表达及其临床意义

李婧博,刘洪彬,贾月月,王泽,孙梅,石玉华   

  1. 山东大学附属生殖医院 国家辅助生殖与优生工程技术研究中心 生殖内分泌教育部重点实验室(山东大学)山东省生殖医学重点实验室, 山东 济南 250001
  • 收稿日期:2016-10-14 出版日期:2017-01-10 发布日期:2017-01-10
  • 通讯作者: 石玉华. E-mail:shiyuhua2003@126.com E-mail:shiyuhua2003@126.com
  • 基金资助:
    国家科技支撑计划(2012BAI32B04);国家自然科学基金面上项目(81471428,81200423);山东省科技发展计划(2014GSF118070);山东省人口和计划生育委员会科技计划(2013-03);山东大学青年学者未来计划(第二批,2016WLJH50);山东大学附属生殖医院自主创新项目(20141102,20141103)

Expression and clinical significance of microRNA-183 in polycystic ovary syndrome with insulin resistance

LI Jingbo, LIU Hongbin, JIA Yueyue, WANG Ze, SUN Mei, SHI Yuhua   

  1. Center for Reproductive Medicine, Shandong University;
    National Research Center for Assisted Reproductive Technology and Reproductive Genetics;
    Key Laboratory for Reproductive Endocrinology(Shandong University), Ministry of Education;
    Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan 250001, Shandong, China
  • Received:2016-10-14 Online:2017-01-10 Published:2017-01-10

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摘要： 目的 检测多囊卵巢综合征(PCOS)胰岛素抵抗患者卵巢颗粒细胞中microRNA-183(miR-183)的表达,并评价其临床意义。 方法 采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)检测120例PCOS患者［其中 78例PCOS胰岛素抵抗患者(PCOS胰岛素抵抗组)和42例PCOS非胰岛素抵抗患者(PCOS非胰岛素抵抗组)及72例正常对照者(正常对照组)］颗粒细胞中miR-183的表达,并对miR-183表达和胰岛素抵抗指数(HOMA-IR)进行关联分析,同时对组间临床资料进行回顾性分析。采用受试者工作特征(ROC)曲线评价miR-183的诊断效能。 结果 miR-183在PCOS胰岛素抵抗组颗粒细胞中的表达高于正常对照组(P<0.001)及非胰岛素抵抗组(P=0.034),PCOS胰岛素抵抗组miR-183的表达与HOMA-IR呈正相关(r=0.476, P=0.003),ROC曲线提示miR-183联合体质量指数(BMI)作为PCOS胰岛素抵抗的评价指标,其诊断效能较高(AUC: 0.820, 95%CI: 0.750~0.889, P<0.001)。 结论 miR-183在PCOS胰岛素抵抗患者颗粒细胞中高表达,并与HOMA-IR相关,其可能通过卵泡局部微环境参与调控PCOS胰岛素抵抗的致病过程。

关键词: 多囊卵巢综合征, 胰岛素抵抗, miR-183, 颗粒细胞

Abstract: Objective To explore the expression of microRNA-183(miR-183)in the ovarian granulosa cells of polycystic ovary syndrome(PCOS)patients with insulin resistance(IR), and to evaluate its clinical significance. Methods A total of 120 PCOS patients including 78 with insulin resistance and 42 without, and 72 healthy controls were enrolled. The miR-183 expression was examined by real-time quantitative reverse transcription PCR(qRT-PCR). The correlation between miR-183 and homeostasis model assessment-insulin resistance(HOMA-IR)was analyzed. The area under the receiver operating characteristic(ROC)curve(AUC)was calculated to evaluate the predictive power of miR-183 for PCOS insulin resistance. Results PCOS patients with insulin resistance had higher miR-183 expression than the control group(P<0.001)and PCOS without insulin resistance group(P=0.034). There was positive correlation 山 东 大 学 学 报 (医 学 版)55卷1期 -李婧博,等.microRNA-183在PCOS胰岛素抵抗中的表达及其临床意义 \=-between HOMA-IR and miR-183 expression in PCOS with insulin resistance group(r=0.476, P=0.003). The ROC analysis suggested that combination of miR-183 and body mass index(BMI)(AUC: 0.820, 95%CI: 0.750-0.889, P<0.001)might be an effective indicator. Conclusion The high expression of miR-183 is correlated to insulin resistance of PCOS and HOMA-IR, and may be involved in the pathogenesis of PCOS insulin resistance through follicular microenvironment.

Key words: MicroRNA-183, Insulin resistance, Granulosa cell, Polycystic ovary syndrome

中图分类号: 

  • R711.75
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