多囊卵巢综合征患者颗粒细胞中microRNA-141的表达与意义

doi:10.6040/j.issn.1671-7554.0.2019.0148

摘要/Abstract

摘要： 目的探讨微小RNA-141(miR-141)在多囊卵巢综合征(PCOS)患者卵巢颗粒细胞中的表达,并评价其临床意义。方法选取70例PCOS患者,根据体质量指数(BMI)分为正常体质量组(BMI<25, n=42)和肥胖组(BMI≥25, n=28);选取同期71例对照患者,分为正常体质量组(BMI<25, n=60)和肥胖组(BMI≥25, n=11)。采用逆转录实时定量PCR(qRT-PCR)检测各组卵巢颗粒细胞中miR-141的表达,并分析其临床意义。结果 PCOS正常体质量组中miR-141的表达高于对照正常体质量组(P<0.001);与对照肥胖组相比,miR-141在PCOS 肥胖组中高表达(P=0.009)。此外,PCOS患者妊娠并发症发生率有升高的趋势,但差异无统计学意义(P>0.0125)。结论 PCOS患者正常体质量组与肥胖组中miR-141的表达水平分别高于其相对应的对照患者,且PCOS患者发生妊娠并发症的风险有升高的趋势,提示miR-141可能在PCOS的发病机制中发挥重要作用。

关键词: 多囊卵巢综合征, 颗粒细胞, microRNA-141, 妊娠并发症

Abstract: Objective To investigate the expression of microRNA-141(miR-141)in ovarian granulosa cells of patients with polycystic ovary syndrome(PCOS)and its clinical significance. Methods A total of 70 PCOS patients were divided into normal-BMI group(BMI<25, n=42) and obesity group(BMI≥25, n=28)according to their(BMI), and 71 controls were divided into normal-BMI group(BMI<25, n=60)and obesity group(BMI≥25, n=11). The expression of miR-141 in ovarian granulosa cells of each group was detected with real-time quantitative reverse transcription PCR(qRT-PCR), and its clinical significance was analyzed. Results The expression of miR-141 was higher in the normal-BMI PCOS group than in the normal-BMI control group (P<0.001); and compared with obesity control group, higher expression of miR-141 was observed in obesity PCOS group (P=0.009). In addition, the incidence of 山东大学学报 (医学版)57卷5期 -贺婷婷,等.多囊卵巢综合征患者颗粒细胞中microRNA-141的表达与意义 \=-pregnancy complications in PCOS patients tended to be higher, but there was no statistical significance(P>0.0125). Conclusion The expression of miR-141 is higher in the PCOS groups than in the control groups. and the risk of pregnancy complications tends to increase in PCOS patients, suggesting that miR-141 may play an important role in the pathogenesis of PCOS.

Key words: Polycystic ovary syndrome, Granulosa cell, MicroRNA-141, Pregnancy complications

中图分类号:

R711.75

贺婷婷,刘红,舒凯云,宋晓翠,石玉华. 多囊卵巢综合征患者颗粒细胞中microRNA-141的表达与意义[J]. 山东大学学报 (医学版), 2019, 57(5): 116-120.

HE Tingting, LIU Hong, SHU Kaiyun, SONG Xiaocui, SHI Yuhua. Expression and significance of microRNA-141 in granulosa cells of patients with polycystic ovary syndrome[J]. Journal of Shandong University (Health Sciences), 2019, 57(5): 116-120.

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