山东大学学报 (医学版) ›› 2019, Vol. 57 ›› Issue (1): 48-54.doi: 10.6040/j.issn.1671-7554.0.2018.726
• • 上一篇
刘聪聪,刘洪彬,符江,李雷
LIU Congcong, LIU Hongbin, FU Jiang, LI Lei
摘要: 目的 探讨多囊卵巢综合征(PCOS)易感基因dennd1a在斑马鱼模型胚胎发育过程以及成鱼不同器官中的表达,构建适用于PCOS分子机制研究的斑马鱼实验动物模型。 方法 采用反转录-聚合酶链反应(RT-PCR)及实时荧光定量逆转录聚合酶链反应(qRT-PCR)技术,检测dennd1a基因在斑马鱼中的表达特性。利用胚胎整体原位杂交(WISH)方法,观察dennd1a基因在斑马鱼胚胎内的表达及分布。 结果 qRT-PCR结果显示斑马鱼dennd1a mRNA的表达水平随着胚胎的发育而增加,且在多个组织中均有表达,于神经系统和雄性生殖系统中存在高表达。WISH结果表明该基因早期主要在神经系统表达。 结论 斑马鱼dennd1a基因的表达在胚胎早期发育中发挥重要作用,基因高表达尤其发现于中枢神经系统和雄性生殖系统,提示该基因的表达可能通过影响神经内分泌功能,调控雄激素的合成或释放,诱发PCOS并导致相关表型。
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