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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (4): 1-5.doi: 10.6040/j.issn.1671-7554.0.2014.666

• 基础医学 •    下一篇

半乳凝素-9抑制胶质瘤细胞增殖及迁移

姚众1, 陈为亮1, 徐洋洋1, 何影2, 曲迅2, 李新钢1   

  1. 1. 山东大学齐鲁医院神经外科 山东大学脑科学研究所, 山东 济南 250012;
    2. 山东大学齐鲁医院基础医学研究所, 山东 济南 250012
  • 收稿日期:2014-10-08 修回日期:2014-12-23 出版日期:2015-04-10 发布日期:2015-04-10
  • 通讯作者: 李新钢.E-mail:lixg@sdu.edu.cn E-mail:lixg@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(81172404,81372720)

Galectin-9 inhibits proliferation and migration of glioma cells

YAO Zhong1, CHEN Weiliang1, XU Yangyang1, HE Ying2, QU Xun2, LI Xingang1   

  1. 1. Department of Neurosurgery, Qilu Hospital of Shandong University, Brain Science Research Institute, Shandong University, Jinan 250012, Shandong, China;
    2. Institute of Basic Medical Sciences, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-10-08 Revised:2014-12-23 Online:2015-04-10 Published:2015-04-10

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摘要： 目的 研究半乳凝素-9(galectin-9)对胶质瘤细胞系U251增殖和迁移能力的影响,并探讨其作用机制.方法 利用实时定量PCR方法检测胶质瘤细胞系中galectin-9的表达情况;加入不同浓度(1、4、8、16 μg/mL)外源galectin-9蛋白处理一定时间(12、24、36 h)后,CCK-8、Transwell实验检测细胞增殖和迁移能力;另外,在galectin-9处理的同时加入其竞争性抑制剂乳糖,观察其对增殖、迁移作用的影响.结果 4种胶质瘤细胞系均极低水平表达galectin-9;8、16 μg/mL 浓度的galectin-9处理组的增殖率较对照组明显降低(P< 0.001),随着时间延长,细胞增殖率逐渐降低(P< 0.01),添加乳糖组细胞增殖率相对于未加乳糖组增高(P< 0.05);外源性galectin-9刺激不同时间,穿至膜下的细胞数较对照组明显减少(P< 0.01),添加乳糖组细胞数较未加乳糖组增多(P< 0.05).结论 半乳凝素-9可明显抑制胶质瘤细胞的增殖,具有浓度和时间依赖性,并且能够有效抑制胶质瘤细胞的迁移.

关键词: 胶质瘤, 迁移, 半乳凝素-9, 增殖

Abstract: Objective To investigate the effects of exogenous galectin-9 on the proliferation and migration of glioma cells and its potential mechanism. Methods The expression of galectin-9 in glioma cells was detected with real-time quantitative polymerase chain reaction (PCR). After glioma cell line U251 was treated with different concentrations of exogenous galectin-9 (1, 4, 8, 16 μg/mL), the changes of proliferation and migration were tested with cell count kit-8 (CCK-8) and transwell chambers respectively at different time (12, 24, 36 h). When the competitive inhibitor lactose was added, the changes in proliferation and migration were detected. Results The expression of galectin-9 in 4 glioma cell lines was low or barely detected. Cells treated with 8 and 16 μg/mL galectin-9 showed decreased cell proliferation rates, compared with control group (P <0.001). With time passing by, the cell proliferation rate decreased gradually (P <0.01). After lactose treatment, cell proliferation rate increased (P <0.05). Galectin-9 treatment significantly reduced the numbers of cells penetrating the micropomus membrane (P <0.01), while lactose treatment increased the number of cells, compared with the untreated group (P<0.05). Conclusion Galectin-9 effectively inhibits proliferation of glioma cells in a time- and dose-dependent manner, and it can also inhibit the migration of glioma cells.

Key words: Proliferation, Migration, Glioma, Galectin-9

中图分类号: 

  • R739.4
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