Table of Content

  

10 September 2013
Volume 51 Issue 9

Articles


Articles

Isolation of rat bone marrow mesenchymal stem cell-derived exosome and the uptake of exosome by H9C2

LIU Shan-wen1, WANG Fu1, LI Bin2, GENG Hai-hua3, LI Cai-e4, XU Zhe5, LI Rui1, XIAO Jie1, ZHANG Sen1, JI Xiao-ping1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  1-7. 

Abstract ( 1149 )   PDF (18020KB) ( 230 )   Save

Related Articles | Metrics

Objective     To study the features such as the diameter distribution and average radius of the hypoxia preconditioned bone marrow mesenchymal stem cells (BMSCs)-derived exosomes; to elucidate whether the exosomes are able to target cardiomyocytes efficiently; to explore the feature of time-dependent uptake of exosomes by H9C2. Methods      We collected exosomes from the supernatant of hypoxia preconditioned BMSCs by the combination of step-by-step centrifugations and ultracentrifugation, characterized exosomes by transmission electron microscopy (TEM) and western blotting, statistically analyzed the messages of the diameters from results of TEM by image analysis software Image Pro Plus 6.0, obtained the distribution of diameter and average radius of exosomes by excel and statistical software Graph Pad 5.0, tracked exosomes with PKH-67, incubated PKH-67-dyed exosomes with H9C2 to observe whether rat BMSCs-derived exosomes could be uptaken by H9C2. Further, we incubated PKH-67-labeled exosomes with H9C2 for different periods to explore the relationship between uptake and time. Results   We observed that the extract was of micro-capsule structures, approximately spherical or ellipsoidal in shape and homogeneous in size. They scattered neatly in the vision, and positively expressed both CD63 and CD9 molecules. The diameters of exosomes approximately ranged from 20nm to 60nm, and the radius was (17.03±0.40) nm. In the incubation experiment of exosomes with H9C2, we could only observe green fluorescent-flashing exosome pellets within the cytoplasm of H9C2 in the dyed positive group. The uptake of exosomes by H9C2 was not different between four subgroups of the control group. For the PKH-67-dyed exosomes within H9C2 cytoplasm 1h after the co-culture, during the period of 1.5h to 2.5h, the numbers of green fluorescent-flashing exosome pellets were more and the fluorescence intensity was stronger than any other experimental subgroups. Conclusion    We successfully isolated hypoxia preconditioned rat BMSCs-derived exosomes, and proved that cardiomyocytes H9C2 were the biological targets of such exosomes. The uptake of exosomes by H9C2 showed significant changes with the extension of incubating time.

Effects of down-regulated α5-nicotinic acetylcholine receptor expression on HIF-1α expression in lung cancer cells

ZU Shan-shan1, MA Xiao-li1,2, JIA Yan-fei1,2, ZHAO Yun3, JIA Ying1, XIAO Dong-jie1,2, WANG Yun-shan1,2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  8-12. 

Abstract ( 1063 )   PDF (1722KB) ( 347 )   Save

Related Articles | Metrics

Objective   To explore the influence of small interfering RNA (siRNA) down-regulated α5-nicotinic acetylcholine receptor (α5-nAChR) expression on hypoxia-induced factor-1α(HIF-1α) expression during the process of nicotine-induced lung cancer A549 cell proliferation in vitro. Methods    Expressions of α5-nAChR and HIF-1α at mRNA and protein levels in A549 cells were detected by FQ-PCR and Western blotting after treatment with various concentrations of nicotine. After CHRNA5-siRNA transfection, nicotine-induced A549 cell proliferation and expression of HIF-1α were detected by MTT, FQ-PCR and Western blotting respectively. Results   Expressions of α5-nAChR and HIF-1α were significantly enhanced with 1μmol/L nicotine treatment compared with the other concentrations(P<0.05), which presented a nicotine dose-dependent manner. After CHRNA5-siRNA transfection, the expressions of α5-nAChR mRNA and protein decreased obviously(P<0.05). Nicotine-induced A549 cell proliferation was significantly inhibited (P＜0.05). Conclusion    α5-nAchR plays an important role in nicotine-induced A549 cell proliferation via regulating expression, which suggests that α5-nAChR/HIF-1α axis may be one of the important molecular mechanisms for nicotine-related lung cancer.

Effects of shear stress on the cytoskeleton in rat pulmonary microvascular endothelial cells

DU Qing-xia1, WANG Chun-xiao1, LI Dong2, XIA Wei1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  13-16. 

Abstract ( 1024 )   PDF (4175KB) ( 210 )   Save

Related Articles | Metrics

Objective     To observe the effects of shear stress on rat pulmonary microvascular endothelial cells (PMVECs) cultured in vitro and to elucidate the early pathogenesis of pulmonary hypertension induced by high pulmonary flow. Methods    Rat PMVECs were cultured with conventional methods. Self-made perfusion system was used to exert different shear stresses on rat PMVECs. The dynamic changes of F-actin stained by FITC-phalloidin were observed through laser scanning confocal microscope. Results   Microfilament rearrangement (compliant changed from former disorderliness into orderliness) occurred in PMVECs due to the shear stress, towards the direction of the shear force. Stress fibers appeared along the cell long axis. This change was time and stress strength dependent. With the extension of treatment time, the phenomena became more obvious， until nearly all the F-actin changed to be stress fibers.  ConclusionThe cytoskeleton of the PMVECs will rearrange to be stress fibers according to the shear stress it endured. This phenomenon is both time and stress strength dependent, and occurs at the early stage. This may be closely related to the early pathogenesis of pulmonary hypertension induced by high pulmonary flow.

Acute hyperglycemia worsens ischemic stroke-induced blood-brain barrier impairment via HMGB1 in rats

HUANG Jing-yang1, YANG Cheng-hui1, MA Kun-run1, LIU Xi1, WANG Yi-fei1, CHEN Hai-li1, LIU Bao-yi2, YUAN Zhong-rui1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  17-21. 

Abstract ( 849 )   PDF (1343KB) ( 234 )   Save

Related Articles | Metrics

Objective   To investigate the effects of acute hyperglycemia on ischemia-induced blood-brain barrier (BBB) impairment and the mechanism involved．Methods   Wistar rats were randomly divided into sham operation group, normoglycemia (NG) group, hyperglycemia (HG) group  and glycyrrhizic acid (GL) intervention (NG+GL, HG+GL)groups. Ischemia was induced by 90 minutes middle cerebral artery occlusion. Western blotting was used to detect the release of high mobility group box 1 (HMGB1) in cerebrospinal fluid, and BBB permeability was evaluated by Evan′s blue (EB) leakage. Brain edema, infarction volume, neurological deficit scores were evaluated after operation. Results   Hyperglycemia significantly enhanced the release of HMGB1 in cerebrospinal fluid, compared with the NG group (P<0.01). Meanwhile, Hyperglycemia significantly increased the brain edema and the infarction volume (P<0.01) and worsened the neurological deficit (P<0.05). Inhibition of HMGB1 with GL significantly reduced EB leakage, brain edema, infarction volume, and neurological deficit (P<0.01). Conclusion    In transient middle cerebral artery occlusion, the increased release of HMGB1 may contribute to the hyperglycemia-exacerbated BBB damage. Inhibiting HMGB1 may protect the BBB from the hyperglycemia-exacerbated damage.

Effect and expression of GRK2 on collagen synthesis in myocardial fibrosis rat model

KAN Li-li1, JIANG Fang-guo1, AN Feng-shuang2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  22-25. 

Abstract ( 1030 )   PDF (1840KB) ( 529 )   Save

Related Articles | Metrics

Objective  To study the role of G protein-coupled receptor kinase 2 (GRK2) on myocardial fibrosis. Methods    Rat model of myocardial fibrosis was established by subcutaneous injection with isoproterenol. Myocardial histological changes of the rats were tested by microscope after hematoxylin and eosin staining (HE). Myocardial fibroblasts were isolated and cultured with differential anchoring velocity. The expressions of GRK2 and collagen type Iα were examined by western blotting. The cAMP level was tested after GRK2 gene was silenced by siRNA. Results   HE staining showed myocardium necrosis, hypertrophy, and lymphocytic infiltration. The expression of GRK2 (P＜0.05) and collagen type Iα (P＜0.05) were up-regulated in isoproterenol-induced rats. The level of cAMP decreased significantly in isoproterenol-induced rats (P＜0.05). However, the level of cAMP increased after GRK2 gene silenced by siRNA (P＜0.05). Conclusion    The intracellular cAMP   increased after GRK2 gene expression is silenced by siRNA. GRK2 may serve as a novel potential therapeutic target.

Effect of Baicalin on atherosclerosis ApoE-/- mice and VE-cadherin level

YIN Hai-peng， LIU Xiang-qun, YU Xin, WANG Min

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  26-30. 

Abstract ( 859 )   PDF (1991KB) ( 288 )   Save

Related Articles | Metrics

Objective   To study the effect of VE-cadherin expressions in the development of atherosclerosis (AS) and the possible mechanism of Baicalin in anti-AS. Methods    ApoE-/- mice model of atherosclerosis (AS) was established. 20 male ApoE-/- mice were randomly divided into the model group and the baicalin treatment group. Ten  C57BL/6 mice were chosen in the normal control group. 50mg/(kg·d) baicalin was given to mice in treatment group, and  physiological saline was given to the mice in AS model group and normal control group for 4 weeks. After 12 weeks， the mice were put to death and the expression level of the serum inflammation biomarkers， aortic NF-κB and VE-cadherin were detected. Results   NF-κB of aortic artery cells in mice, VE-cadherin positive expression and inflammatory factor levels  were obviously increased compared with the normal group (P<0.01 or P<0.05). After the treatment of baicalin, aortic artery cells NF-κB in mice, VE-cadherin expression and inflammatory factors in the treatment group were significantly reduced (P<0.01 or P<0.05), which showed that VE-cadherin expression,  the NF-κB expression and inflammatory factor levels were positively related. Conclusion    Baicalin could have certain protective effect and the mechanism may be related to  reduction the NF-κB transcription activity, lower inflammatory factors and VE-cadherin, thereby reducing the injury of endothelial cell.

Construction and effect of shRNA vector silencing EZH2 expression

Lv Yan-feng1, HAN Bing-bing2, YU Hua-long1, WANG Jian-xin1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  31-34. 

Abstract ( 981 )   PDF (1366KB) ( 362 )   Save

Related Articles | Metrics

Objective     To construct a recombinant short hairpin RNA (shRNA) expression vector targeting enhancer of zeste homolog 2(EZH2) gene and explore its effect on colorectal cancer cells. Methods    Two DNA sequences with short hairpin structure were designed according to the EZH2 cDNA sequence and cloned into pGFP-V-RS vector to construct a recombinant expression vector silencing EZH2 gene.After identification, the shRNA-expressing vector was then transfected into SW480 cells.Then transfected SW480 cells were divided into negative control group and gene silencing group.RT-PCR and Western blotting were used to detect inhibitory effect.MTT was used to detect cell proliferation. Results   A recombinant vector harboring shRNA of EZH2 was successfully constructed in this work.With respect to assessment of interference efficiency,the expression of EZH2 mRNA in negative control group was 5.8 times (P<0.01) of that in gene silencing group. And the expression of EZH2 protein in gene silencing group was significantly lower than that in negative control group (P<0.05 ).Moreover, the cell viability in gene silencing group was significantly reduced than that in negative control group (P<0.05). Conclusion    The present study demonstrates that a recombinant shRNA expression vector targeting EZH2 gene was successfully constructed, with significant inhibitory effect on proliferation of SW480 cells. This lays an experimental foundation for further exploring the mechanism underlying the action of EZH2 gene on tumor biology.

Inhibition of epithelial-mesenchymal transition by metformin in  prostate cancer cells and correlative mechanisms

ZHANG Jing1， WANG Lin2，3， XIA Ping-tian3， QIN Xiao-min4， HAN Bo3，4， GUO Xiu-li1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  35-39. 

Abstract ( 1231 )   PDF (24439KB) ( 206 )   Save

Related Articles | Metrics

Objective   To investigate the effects of metformin on proliferation, invasion and epithelial-mesenchymal transition (EMT) of prostate cancer Vcap cells and the possible miRNA-based mechanisms. Methods    Vcap cells that were treated with PBS were used as control group. MTS assay was used to determine cellular proliferation of Vcap cells treated by metformin with various concentrations (1-50mmol/L). Flow cytometric analysis was performed to detect cell cycle distributions. Wound healing assay and martrigel invasion assay were performed to evaluate invasive capacity of cancer cells treated by 5mmol/L metformin and miR30a; RT-PCR and Western blotting were used to detect mRNA and protein expression levels of epithelium markers (β-catenin, E-cadherin) as well as mesenchymal marker (Vimentin, N-cadherin). RT-PCR was used to detect the expression levels of miR30a, miR143, miR185, miR196b and miR205. Results   Metformin significantly inhibited proliferation of Vcap cells in a dose- and time-dependent manner. 5mmol/L metformin significantly influenced cell cycle distribution and inhibited invasiveness and motility capacity of Vcap cells. Metformin upregulated expression of E-cadherin (P<0.05) and β-catenin (P<0.05), but downregulated Vimentin (P<0.05) and N-cadherin (P<0.05) expression at mRNA and protein levels in Vcap cells. Significant upregulation of miR30a expression levels by metformin was identified (P<0.05) and further experiments confirmed that miR30a significantly inhibited proliferation and EMT of Vcap cells. Conclusion    Metformin significantly inhibits cell proliferation, invasion and EMT in prostate cancer Vcap cells. This process may involve upregulation of miR30a by metformin.

Inhibition of Salinomycin on the characteristics of colon cancer stem cell

SUN Xiu-mei1, GAO Feng2, LIU Zi-feng3

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  40-44. 

Abstract ( 925 )   PDF (2220KB) ( 262 )   Save

Related Articles | Metrics

Objective   To investigate the impacts of Salinomycin on colon cancer stem cell (cCSC). Methods    The cCSC was screened by culturing the clone spheres in Serum Free Medium (SFM). Further, we detected the targeting of Salinomycin to cCSC and studied the impacts of Salinomycin on the characteristics of cCSC including proliferation ability, drug resistence, clone sphere forming ability, expression level of stem cell proteins. Results   We acquired cCSC successfully by sphere culturing in SFM and established the ideal cCSC model. The cCSC showed triple sensitivity to Salinomycin than HCT116+/+ cell. After treatment by Salinomycin, the double time of cCSC increased from (1.21±0.15) days to (1.54±0.04) days; the IC50 to 5-FU declined from (40.21±3.02) μmol/L to (20.28±2.15) μmol/L, and the IC50 to oxaliplatin declined from (14.23±1.08) μmol/L to (7.64±0.53) μmol/L; the clone sphere forming rate declined from (0.42±0.01)% to (0.08±0.01)%, and the expression level of ABCG2, SOX2 and OCT4 also declined significantly. Conclusion    Salinomycin possesses significant targeting to cCSC, and can inhibit the proliferation ability, drug resistence, clone sphere forming ability, expression level of stem cell proteins of cCSC. This provides new theory basis for cCSC therapy.

Construction of STAT3 eukaryotic over-expression vector and its effects on the proliferation of gastric cancer cells

WANG Xiao-lei1, JIA Yan-fei2, ZHENG Yan2, LIU Hua2, WANG Yun-shan2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  45-49. 

Abstract ( 858 )   PDF (1397KB) ( 241 )   Save

Related Articles | Metrics

Objective     To investigate the effects of overexpression human signal transducer and activator of transcription 3(STAT3) on human gastric cancer cell line MGC803 by the construction of a STAT3 gene eukaryotic expression vector. Methods    The eukaryotic expression vector pcDNA3-STAT3 was constructed and transfected into the gastric cancer cell line（MGC803) by the liposome. The mRNA and protein expression levels of STAT3, P-STAT3(Y705), P-STAT3(S727), Survivin and PCNA in MGC803 were detected by RT-PCR and Western blotting. The effects of STAT3 on MGC803 cell proliferation were detected through the CCK-8 assay. Results   The DNA sequencing and restriction endonuclease identification results confirmed that the eukaryotic expression plasmid pcDNA3-STAT3 was successfully constructed. Western blotting and RT-PCR results confirmed that STAT3, P-STAT3(Y705), P-STAT3(S727), Survivin and PCNA protein and mRNA expression levels were significantly increased in the recombinant plasmid group compared with the empty vector group. CCK-8 results proved that, compared with the empty vector group，the recombinant plasmid group showed higher proliferative capacity, and the difference was statistically significant (P<0.05). Conclusion    eukaryotic expression plasmids of pcDNA3-STAT3 can increase the expression of STAT3 gene in human gastric cancer cell line MGC803, and enhance the proliferation of gastric cancer cells.

Effect of basil polysaccharide on the invasiveness of MDA-MB-231  cells

SHI Huan1,2, SHAO Qian-qian1, SUN Jin-tang1, SONG Bing-feng1, Lv Jing1,3, HE Ying1, ZHANG Yun1, QU Xun1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  50-54. 

Abstract ( 924 )   PDF (1831KB) ( 276 )   Save

Related Articles | Metrics

Objective     To investigate the effect of basil polysaccharide on the invasiveness of MDA-MB-231 cells. Methods    MDA-MB-231 cells cultured in vitro were divided into the experimental group and control group. The control group was not treated, while the experimental group was treated with 100μg/mL or 300μg/mL basil polysaccharide. The invasiveness of the cells was detected with transwell assay. The expressions of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and membrane-type 1 matrix metalloproteinase (MT1-MMP) were tested with RT-PCR and Western blotting. Results   Basil polysaccharide suppressed the invasion of MDA-MB-231 cells (P<0.01), decreased the mRNA expression of MT1-MMP (P<0.05), but had no effect on the mRNA expression of MMP-2 and MMP-9. Conclusion    Basil polysaccharide inhibits the invasiveness of MDA-MB-231 cells by reducing the expression of MT1-MMP.

Protective effect of bone marrow mesenchymal stem cells and CD133+ renal cells on acute renal injury

GE Jun-ke, ZHAO Sheng-tian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  55-59. 

Abstract ( 882 )   PDF (2002KB) ( 388 )   Save

Related Articles | Metrics

Objective   To investigate the protective effect of exogenous bone marrow mesenchymal stem cells (MSCs) and CD133+ renal cells in acute renal injury induced by ischemia/reperfusion. Methods    A total of 32 male C57BL/6 mice were divided into the normal control group, I/R group, I/R+ MSCs group and I/R+ CD133+ renal cell group, with 8 mice in each group. On the first, second, third and seventh day after the models were established, 2 mice in each group were sacrificed respectively. The blood was sampled to detect the level of blood urea nitrogen (BUN) and creatinine (Cr). The kidneys were prepared for paraffin sections, the pathological changes were observed, and the acute tubular necrosis (ATN) score of kidney tissue was evaluated. The levels of tumor necrosis factor-α (TNF-α) and hepatocyte growth factor (HGF) in kidney homogenate were detected by enzyme-linked immune sorbent assay (ELISA). Results   ① The levels of the BUN, Cr, TNF- α and ATN score of the I/R+MSCs group were higher than those of the control group while lower than those of the I/R group, with the most significant difference on the 7th day after operation (P<0.05). The HGF level of the I/R+MSCs group was lower than that of the control group while higher than that of the I/R group, with the most significant difference on the 7th day after operation (P<0.05). ②The levels of the BUN, Cr, TNF-α and ATN score of the I/R+CD133+ renal cell group were higher than those of the control group while lower than those of the I/R group, with the most significant difference on the 3rd day after operation (P<0.01). The HGF level of the I/R+CD133+ renal cell group was lower than that of the control group while higher than that of the I/R group, with the most significant difference on the 3rd day after operation (P<0.01). ③ The levels of the BUN, Cr, TNF-α and ATN score of I/R+CD133+ renal cell group were lower than those of the I/R+MSCs group, while the HGF level was higher (P<0.05). Conclusion    Exogenous bone marrow MSCs and CD133+ renal cells may regulate cytokine secretion and improve microenvironment in renal injury by endocrinic approach, which helps the recovery of acute renal injury, and the CD133+ renal cells may be more effective than MSCs.

Expression of autophagy-related gene Beclin1 on second-degree scald skin in rats

ZHU Xu-guo, LIU Lei, JIANG Du-yin, YU Guan-ying, LI Chuan, LI Yao-nan, LIU Zhen-zhong, ZHANG Ji-xun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  60-63. 

Abstract ( 991 )   PDF (4826KB) ( 310 )   Save

Related Articles | Metrics

Objective     To observe expression of autophagy-related gene Beclin1on second-degree scald tissues in rats, and to investigate the relationship of autophagy and scald tissues. Methods    Sixteen  Wistar rats were randomly divided into 2 groups:the normal group (the control group) and the scald group (the experimental group), 8 rats in each group. Second-degree scald models were established in experimental group, and scald tissues were obtained on day 1, 3 and 7 after scald. The rat skin in each group was evaluated by histologically examination. Expression of Beclin1 in each group was detected by the immunohistochemiscal technique and conducted by image processing techniques. Statistical analyses were performed. Results   Expression of Beclin1 was positive in the experimental group, but negative in the control group(P＜0.05). Conclusion    Expression of Beclin1 in the experimental group was significantly higher than that in the control group, and autophagy was speculated to participate repair process of second-degree scald.

Detection of the Nelin in peripheral blood of patients with varicose veins of lower extremity and its significance

PEI Chang-an, QIN Shi-yong, CHEN Shi-hui, ZHANG Shu-guang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  64-66. 

Abstract ( 854 )   PDF (964KB) ( 366 )   Save

Related Articles | Metrics

Objective     To explore the different expressions and significance of Nelin in the peripheral blood of healthy controls and patients with varicose veins of lower extremity. Methods    A total of 57 patients with varicose veins of lower extremity treated in our hospital and 60 healthy controls were enrolled in the study. The expression of Nelin mRNA in the peripheral blood was detected with RT-PCR; Nelin protein in the peripheral blood was determined with double-antibody sandwich ELISA technique. Results   The results of RT-PCR showed that the expressions of Nelin mRNA in patients and controls were 0.226±0.070 and 0.718±0.114 respectively (t=9.028, P<0.01), with statistical difference.  ELISA examination showed that Nelin protein was detectable in all serum samples, which was (2.61±1.73) ng/mL in patients and (3.64±1.65)ng/mL in controls (t=2.92, P<0.01). The difference was statistically significant. Conclusion    The expression of Nelin in patients with varicose veins is significantly lower than that in healthy controls at both transcription and translation levels, indicating that the expression of Nelin is related to the pathogenesis and development of varicose veins of lower extremity.

Differential procedure of narrow QRS complex tachycardia based on lead V1 and avL in electrocardiograms

LIU Wen-ting, LIU Tong-bao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  67-71. 

Abstract ( 1091 )   PDF (1095KB) ( 317 )   Save

Related Articles | Metrics

Objective   To propose a set of differential procedure of narrow QRS complex tachycardia, based on pseudo r′ wave in lead V1 and terminal notching of QRS complex in lead avL, to observe and evaluate the clinical significance.   Methods   We randomly selected 277 in-patients with proximal supraventricular tachycardia who received radiofrequency ablation; observed the electrocardiograms of both sinus rhythm and tachycardia induced during the surgeries in 165 patients, including 90 in the atrioventricular nodal reentrant tachycardia (AVNRT) group, and 75 in the orthodromic atrioventricular reciprocating tachycardia (AVRT) group; proposed a set of differential procedure according to the sensitivity, specificity, PPV and NPV of differential algorithms. We then carried out a blinded prospective research on the other 112 patients’ electrocardiograms, and evaluated the accuracy of the procedure. Results   The following algorithms were of significant differences between the two groups (P<0.005): pseudo r′ wave in lead V1, terminal notching of  QRS complex  in lead avL, RP interval longer than 70ms, ST segment elevation in lead avR and depression over 0.2mV in precordial leads. With the new differential procedure, we could correctly diagnose 91.38% of AVNRT and 88.89% of AVRT. Conclusion    The differential procedure based on pseudo r′ wave in lead V1 and terminal notching of QRS complex in lead avL can improve the accuracy of differential diagnosis between slow-fast AVNRT and overt AVRT.

Meta-analysis of postoperative effects of dynamic neutralization system (Dynesys) compared with posterior lumbar interbody fusion (PLIF)

WANG Bing-xiang1, NIE Lin1, PU Hua-qing2, ZHANG Zhi-mian2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  72-78. 

Abstract ( 989 )   PDF (3787KB) ( 298 )   Save

Related Articles | Metrics

Objective     To assess the postoperative effects of two different surgical methods—Dynesys and posterior lumbar interbody fushion(PLIF). Methods    Literatures about postoperative effects of Dynesys compared with PLIF from 1994 to 2013 were collected. Stata SE 11.2 software was applied to analyze the included literatures. Results   A total of 359 cases (159 cases for Dynesys, 200 cases for PLIF) in 7 literatures were included. The SMD and 95%CI of the related indicators were as follows:operation time, -1.34(-1.95—-0.73); bleeding volume, -1.28(-2.18—-0.38); postoperative VAS, 0.03(-0.35—0.41); postoperative ODI, -0.07(-0.30—0.17); operated segment ROM, 4.57(3.29—5.85); and adjacent segment ROM, -1.36(-2.32—-0.40). Conclusion    The results indicate that Dynesys has significant advantage on decreasing operation time and bleeding volume. There is  no significant difference in postoperative VAS and ODI between the two surgical methods. Dynesys can maintain mobility of operated segment without the ROM of adjacent segment increased.

Quantitative analysis of 3.0T MR in differential diagnosis of breast lesions

MA Zhen-shen1, WANG Da-wei2, SUN Xiu-bin3, SHI Hao1, PANG Tao1, DONG Gui-qing1, ZHANG Cheng-qi1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  79-83. 

Abstract ( 949 )   PDF (2994KB) ( 381 )   Save

Related Articles | Metrics

Objective     To study the value of 3.0T MR quantitative assessment analysis in the diagnosis of breast lesions. Methods    44 patients with breast lesions were selected. All the patients underwent MR plain scanning and T1 dynamic contrast-enhanced imaging. The vascular function parameters (Ktrans, Kep, Ve and iAUC) of the lesions were acquired. These parameters were compared in benign and malignant breast lesions, and also compared in differential grades of invasive ductal carcinoma. Results   ①The value of Ktrans, Kep and iAUC between the benign and malignant tumors had significant difference, and the value of Ve between the benign and malignant tumors had no significant difference. ②The values of Ktrans, Kep and iAUC in invasive ductal carcinoma between grade I and grade II, and between grade I and grade III had significant difference, and there was no significant difference between grade II and grade III. The value of Ve in invasive ductal carcinoma among grade I, grade II and grade III had no significant difference. ③Ktrans was more meaningful in the differentiation of benign and malignant lesions. Conclusion    3.0T MR quantitative assessment analysis is valuable in the diagnosis of benign and malignant breast lesions. It can also provide reference value in the differentiation of histological grade of breast invasive ductal carcinoma.

Surgical treatment for malignant focal ground glass opacity lung nodule

ZHANG Lin1, ZHANG Zhen-jiang2, MENG Long1, MA Wei1, DU Jia-jun1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  84-87. 

Abstract ( 936 )   PDF (1218KB) ( 323 )   Save

Related Articles | Metrics

Objective     To identify clinical and pathological characteristics of malignant focal ground glass opacity lung nodule (fGGON) and to select suitable candidates for curative limited resection according to the proportion of GGO. Methods    A retrospective analysis of 96 consecutive patients who underwent a complete resection for malignant fGGON was performed during the past three years. All patients were classified as three groups according to the proportion of GGO showed on preoperative CT. The pathological type and prognosis were compared among those groups. The relationships between invasive pathological type and other clinical factors were also assessed. Results   Most of malignant fGGON was adenocarcinoma in early stage. With the increase of GGO component in lung cancer, the proportion of simple bronchioloalveolar cell carcinoma (BAC) also increased (P<0.05). There was no invasive pathological type in patients with pure GGO component, but 28.1% and 44.4% were found to have invasive pathological character in those with high GGO component and low component respectively (P<0.01).The overall 3-year survival rate was 100% in general. The 3-year tumor-free survival rate was 100% for patients with pure GGO component, whereas it was 90.6% for those with high GGO component and 69.4% with low GGO component (P<0.01). Conclusion    The prognosis after operation for malignant fGGON was excellent. The index of GGO component has significant correlations with histological classification, pathologic invasiveness, and postoperative outcome of malignant fGGON. Patients with tumors that have pure GGO component are considered to be possible candidates for limited pulmonary resection.

A comparison of lipid metabolism in PCOS patients with different testosterone levels in Ningxia Hui Autonomous Region

HA Ling-xia1,2, SHI Yu-hua1, ZHAO Jun-li2, CHEN Zi-jiang1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  88-91. 

Abstract ( 1035 )   PDF (870KB) ( 305 )   Save

Related Articles | Metrics

Objective   To explore the effect of hyperandrogenemia on lipid metabolism in patients with polycystic ovary syndrome (PCOS) in Ningxia Hui Autonomous Region. Methods    A total of 267 female patients with PCOS (178 Han and 89 Hui nationalities) were collected in this study, who were divided into high androgen group and normal androgen group according to   their blood testosterone levels. And then their clinical characteristics of hyperandrogenemia were analyzed and compared. Results   ① In all ethnic patients, in Hui and Han patients, the incidence of acne was 20.60%, 16.85% and 22.47%; the incidence of hirsutism was 25.09%, 21.35% and 26.97%; the incidence of hyperandrogenemia was 47.56%, 50.56% and 46.06%, respectively. Hirsutism was more common in Han group than in Hui group (P<0.01). ② In all ethnic patients, the total cholesterol (CHO) and apolipoprotein (ApoB) were significantly higher in the hyperandrogenemia group than in normal androgen group (P<0.05). In Han patients, CHO, low-density lipoprotein (LDL) and ApoB were significantly higher in the hyperandrogenemia group than in normal androgen group (P<0.05 or P<0.01). In Hui patients, there was no significant difference between the two groups (P>0.05). ③ In the non-obesity Han group with non-IR, CHO and ApoB were significantly higher in the hyperandrogenemia group than in normal androgen group (P<0.05). Conclusion    Hyperandrogenemia is the risk factor for dyslipidemia in women with PCOS, independent of obesity and insulin resistance. However, there exist differences between Hui and Han groups.

The role of serum miR-146a in early diagnosis of sepsis

LIU Xin-nong, ZHAN Han-xiang, HU San-yuan, ZHANG Guang-yong, WANG Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  92-94. 

Abstract ( 948 )   PDF (1491KB) ( 188 )   Save

Related Articles | Metrics

Objective     To evaluate the role of serum miR-146a in early diagnosis of sepsis. Methods    From January 2012 to February 2013, serum samples from patients diagnosed with sepsis in intensive care unit (ICU), SIRS in general surgery and healthy controls in Qilu HosPital of Shandong University were collected and their data about age, gender, body mass index (BMI) were recorded at the same time. The expression levels of serum miR-146a in all subjects were determined by Taqman-probe real-time quantitative PCR and the diagnostic performance of serum miR-146a was analyzed by Receiver Operating Characteristic Curve (ROC). Results   Serum miR-146a was significantly reduced in group A compared to the patients in group B and C (P＜0.05) and the sensitivity, specificity and area under the receiver operating characteristic curve were 99.0%, 68.0%, 0.82, respectively. In addition, the levels of serum miR-146a in the patients with sepsis was lower than those in the patients with SIRS (P＜0.05). Conclusion    Serum miR-146a could serve as a new diagnostic biomarker for sepsis with higher sensitivity and specificity. What′s more, it could distinguish sepsis from SIRS.

Changes in mandibular direction during and after protraction headgear treatment

WANG Na-na, CAO Cong, LIU Dong-xu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  95-99. 

Abstract ( 800 )   PDF (1198KB) ( 777 )   Save

Related Articles | Metrics

Objective     To observe and evaluate the mandibular changes in growing Class Ⅲ malocclusion patients treated with protraction headgear. Methods    This study consisted of 30 adolescent Class Ⅲ malocclusion subjects treated with protraction headgear and 30 untreated controls from the Department of Orthodontic, School of Stomatology, Shandong University. All the subjects had their lateral cephlometrics taken before and after the treatment or observation period.The cephalometric data including variables assessing sagittal and vertical relationships of maxillary was analyzed and the direction of mandibular displacement was evaluated according to a structural superimposition method. Results   The results showed significant improvements in dentofacial profile after 6 months of maxillary protraction. During the treatment or observation period, the mean mandibular rotation between the treatment and control groups had significant difference (P<0.05). It was clockwise rotation for the treatment group and anticlockwise rotation for the control group. The mean rotation in the treatment and control groups were 0.8° and -0.6°, respectively. A more horizontal movement of the mandibular could be observed in the control group during the study period. Conclusion    The mandibular of the treatment group produces clockwise rotation while the control group has a trend of anticlockwise rotation after protraction headgear treatment.

Information processing speed and related factors in first-episode schizophrenia

YANG Li-min1,2, WANG Yan1, SUN Meng-meng2, CUI Kai-yan2, WANG Li-na2, LIU Lan-fen1,2

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  100-104. 

Abstract ( 982 )   PDF (872KB) ( 393 )   Save

Related Articles | Metrics

Objective   To explore the information processing speed and the related factors in first-episode schizophrenia. Methods    Information processing speed of 79 cases of first-episode schizophrenia and 54 healthy controls was assessed with the matrics consensus cognitive battery(MCCB). The clinical symptoms of patients were evaluated with the positive and negative syndrome scale (PANSS). Results   The patients′ information processing speed, their scores in trail making test, symbol coding and verbal fluency test were significantly lower than those of the controls. Gender, age of first onset, family history and course of disease were closely related to the information processing speed in first-episode schizophrenia. Conclusion    The information processing speed of cognitive function in first-episode schizophrenia is  significantly impaired. Age of first onset, family history, gender and course of disease are  associated with the processing speed in first-episode schizophrenia.

Relationship between TCM college students’ IAD cases and the five-state personality theory

ZHANG Bo-hua, HU Shuang, SHENG Jie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  105-108. 

Abstract ( 766 )   PDF (864KB) ( 340 )   Save

Related Articles | Metrics

Objective   To explore the incidence of internet addiction disorder (IAD) in the students who study at the schools of Traditional Chinese Medicine and its connection with five-state personality. Methods    The IAD group and the control group were selected to study by CIAS. Results   52 IAD students in 853 college students were selected. The incidence of IAD in the samples is 5.51%. Scores of Taiyang and Shaoyang in the IAD group were significantly lower than those of the control group (P＜0.01), while scores of Taiyin and Shaoyin were significantly higher than those of the control group (P＜0.01); scores of each IAD factor were positively correlated with the score of Taiyin factor (P＜0.01). Compulsive Internet behaviors, tolerance of Internet addiction, social and health issues scores were positively correlated with that of Shaoyin factor (P＜0.01). When Taiyin and Shaoyin entered the regression equation (F=9.903，P＜0.01), they had obvious effect to predict IAD. Conclusion     Personality of Taiyin and Shaoyin is closely related to IAD. It also indicates that Taiyin and Shaoyin scores can predict IAD.

Analysis of burden of family long-term caregivers for disabled elders in Jinan city and influencing factors

LI Ming1, YANG Hua2, GAO Qian-qian1, LI Shi-xue1

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(9):  109-112. 

Abstract ( 947 )   PDF (861KB) ( 426 )   Save

Related Articles | Metrics

Objective   To analyze the burden of family long-term caregivers for disabled elders and its influencing factors. Methods    Using stratified random cluster sampling, 418 disabled elders and their family caregivers were chosen and interviewed with Zarit burden interview (ZBI), Barthel index, instrumental activity of daily living（IADL） and caregiver task inventory (CTI), respectively. Results   The average score of burden of caregivers for disabled elders was 40.97. Nearly 85.4% caregivers had different level burden. Elders with high IADL and Barhel index, good health status and having spouse decreased the burden of caregivers（P<0.01）. High ability to care and having helpers decreased the caregivers’ burden（P<0.01）. Conclusion    The burden of family long-term caregivers for disabled elders is serious. The disabled elders’ ADL score，health status, age, whether having spouse, the ability of caregivers and whether having helpers are associated with the burden of caregivers.