Loading...

Table of Content

      
    10 August 2013
    Volume 51 Issue 8
    Articles
    Role of ALK7 in the transformation of cardiac fibroblasts and the production of collagen type I induced by high glucose
    SHANG Rui1,2, TANG Meng-xiong2,3, LIU Lin1,2, DING Wen-yuan2,3, HAO Pan-pan2,3, CHEN Yu-guo2,3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  1-6. 
    Abstract ( 1305 )   PDF (7401KB) ( 740 )   Save
    Related Articles | Metrics

    Objective   To investigate the role of ALK7 in the process of myocardial interstitial fibrosis (transformation of cardiac fibroblasts and the production of collagen type I by cardiac fibroblasts) induced by high glucose. Methods   Neonatal rat cardiac fibroblasts were cultured in vitro with different concentrations of glucose. Fibroblast phenotype transformation was observed by immumofluorescence method.The mRNA expressions of collagen type I and ALK7 were measured by real timePCR; the protein level of collagen type I was determined by enzymelinked immunosorbent assay; the protein level of ALK7 was measured by Westernblotting analysis. Thereafter, we investigated the theraputic effects of ALK7 siRNA on the above changes induced by high glucose. Results   The transformation of cardiac fibroblasts to myofibroblast increased by high glucose; Both mRNA and protein expressions of collagen type I increased after high glucose stimulation (P<0.05); The mRNA and protein expressions of ALK7 increased by high glucose (P<0.05); The increased mRNA and protein levels of collagen type I and the transformation of cardiac fibroblasts induced by high glucose were reversed by treatment with ALK7 siRNA (P<0.05). Conclusion   ALK7 is involved in the transformation of cardiac fibroblasts to myofibroblast and the production of collagen type I by cardiac fibroblasts induced by high glucose.

    Osteogenic potential of bone marrow mesenchymal stem cells from streptozotocininduced diabetic rats
    HU Su1,2, PANG Shu-guang2, CUI Ying2, YU Chun-xiao1, ZHAO Jia-jun1, GUAN Qing-bo1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  7-12. 
    Abstract ( 841 )   PDF (3552KB) ( 262 )   Save
    Related Articles | Metrics

    Objective   To explore the proliferation, anti-apoptosis characteristics and osteogenic differentiation of BMSCs isolated from diabetic rats. Methods   Forty 6-week female adult Sprague-Dawley rats were randomly divided into the control group and experimental group, with 20 animals in each group. The rats in the experimental group were administrated with a single intraperitoneal injection of streptozotocin (STZ, 65mg/kg, dissolved in 5mmol/L citrate buffer, pH 4.5) to induce diabetes mellitus. The control group was treated with the same dose of normal saline. After 10-week treatment, BMSCs were isolated from the two groups by using adherent method. The proliferation of secondpassage BMSCs was evaluated by CCK-8 assay. The apoptosis induced by serum deprivation and 0.3% hydrogen peroxide was assessed by flow cytometry. After 4-week osteoinductive culture, the expressions of COL-I, OCN, and Runx-2 mRNA were detected by RT-PCR. Alkaline phosphatase (ALP) activity was determined in cell lysates using enzyme-linked immunosorbent assay (ELISA). Meanwhile, the capacities of mineralization of BMSCs were measured by VonKossa and alizarin red staining. Results   Diabetic rat models were successfully induced by intraperitoneal injection of STZ. The proliferative and antiapoptotic abilities of BMSCs derived from diabetic rats were significantly decreased compared with those from normal rats (P<0.01). The mRNA expressions of COL-I, OCN and Runx-2 were down-regulated in experimental group. ALP activity in the experimental group was lower than that in the control group. In addition, the ability of osteogenic differentiation decreased significantly. Conclusion   BMSCs derived from diabetic rats show an impaired ability of proliferative, anti-apoptosis and osteogenic differentiation in vitro.

    Protection against ischemiareperfusion injury in aged liver donor by induction of exogenous hTERT gene
    JIN Bin1, WANG Wei2, LIU Ze-yang1, LI Guang-zhen1, DU Gang1, ZHANG Zong-li1, HAN Li-tao1, HUANG Guo-zhen1, TANG Zhen-yu1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  13-16. 
    Abstract ( 992 )   PDF (10069KB) ( 252 )   Save
    Related Articles | Metrics

    Objective   To investigate the liver ischemiareperfusion (I/R) injury of adult and aged rats. Exogenous human telomerase reverse transcriptase (hTERT) gene was transferred into aged rats′ liver before liver transplantation, and then the effects of the gene on cell apoptosis caused by I/R injury were observed. Methods   Wistar rats were divided into two groups, with adult rats (5 months) in group Ⅰ and aged rats (16~18 months) in group Ⅱ. After transplantation, ALT content, chronic oxidative stress, lipid peroxidation related indicators including vitamin C and vitamin E, the content of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) were tested. The aged rats were divided into 3 groups: group A were pretreated with exogenous hTERT gene, group B with adenovirus vector and group C with physiologic saline. The indicators were detected to analyze the effects of exogenous hTERT gene on I/R injury. Results   Contents of vitamin C, vitamin E, SOD, CAT were lower in group Ⅱ than in group Ⅰ (P<0.05); MDA and ALT were higher in group Ⅱ than in group Ⅰ (P<0.05). The apoptotic index and ALT level were significantly lower in group A than in group B and C (P<0.05), while telomerase activity was increased and histological injury was milder in group A. Conclusion   Compared with that in the adult rats, the I/R injury in aged liver donors were severer. Exogenous hTERT gene induction offers protection against I/R injury in aged liver.

    Effects of resveratrol on brain inflammation and the level of Aβ1-42 in hippocampus from ovariectomized obese rats fed with a high-fat diet
    MIAO Yu-lian, WU Chuan-long, LIU Jin-bo, ZHANG Xiao-li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  17-21. 
    Abstract ( 894 )   PDF (1747KB) ( 301 )   Save
    Related Articles | Metrics

    Objective   To explore the effects of resveratrol on the neuroinflammation and the level of Aβ1-42 in hippocampus from ovariectomized obese rats fed with a highfat diet. MethodsThirty female Wistar rats aged 3 months were randomly divided into three groups: the sham operation fed with low-fat diet (SHAM) group; the ovariectomy fed with high-fat diet(OH) group; the ovariectomy plus highfat diet treated with resveratrol [40mg/(kg·d)-1] (OHR) group. After three months of administration, the rats blood was collected from arteria cruralis to detect the serum concentration of total TC, TG, LDLC, HDLC and E2; the expression of IL6 and TNFα mRNA in hippocampus was determined by realtime PCR; the levels of IL6, TNFα and Aβ142 protein in hippocampal tissues were observed by ELISA. ResultsThe level of serum E2 was significantly lower in the OH group than that in the SHAM group(P<0.01). However, rats in the OHR group had a higher level of serum E2 than rats in the OH group (P<0.05); The levels of serum TC and LDLC in the OH group were higher than those in the SHAM group (P<0.01), and rats in the OHR group had a lower level of serum TC(P<0.01); Expression of IL6, TNFα mRNA and IL6, TNFα, Aβ142 proteins in the OH group significantly increased compared with that in the SHAM group (P<0.01). Nevertheless, rats in the OHR group showed significantly lower expression of IL6, TNFα mRNA and IL6, TNFα, Aβ142 proteins rats in the OH group (P<0.05). ConclusionResveratrol can attenuate the accumulation of Aβ142 in hippocampus from ovariectomized obese rats fed with a highfat diet. The mechanism may be associated with its increasing the level of E2, decreasing the content of TC and ameliorating the obesityrelated brain inflammatory responses.

    Effect of glucagon-like peptide-1 on the levels of FABP-4 and CPT-1A  during the process of 3T3-L1 preadipocyte differentiation
    YANG Jun-peng1,2, HOU Xin-guo1,2, SONG Jun1,2, WU Chuan-long1,2, LI Wen-juan1,2, GONG Lei1,2, YAN Fei1,2, WANG Ling-shu1,2, CHEN Li1,2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  22-27. 
    Abstract ( 1189 )   PDF (13156KB) ( 183 )   Save
    Related Articles | Metrics

    Objective   To examine the effect of glucagon-like peptide-1 (GLP-1) on the levels of fatty acid binding protein 4 (FABP-4) and carnitine palmitoyltransferase 1A (CPT-1A) during 3T3-L1 preadipocyte differentiation process. Methods   In this paper, the traditional “Cocktail method” was adopted to induce the 3T3-L1 preadipocyte differentiation and 1×10-8 mol/L GLP-1 was added to the medium. At the 6th day and the 8th day of differentiation, Oil Red O staining was used to examine the lipid accumulation; RT-PCR and Western blotting were employed to test the levels of FABP-4 and CPT-1A. Results   ①It is found that about forty percent of the cells appeared lipid droplets at the 6th day; about eighty percent of the cells had differentiated to mature adipocytes at the 8th day. ②The results of Oil Red O staining showed that the lipid accumulations in GLP-1 group were slightly increased at the 6th day while modestly decreased at the 8th day (both P>0.05), as compared to the control group. ③RTPCR results indicated that the mRNA level of FABP-4 in GLP-1 group was significantly increased at the 6th day and the 8th day. And the mRNA level of CPT-1A was also enhanced at the 8th day (P<0.05). ④ Western blotting results showed that the protein expression of FABP-4 in GLP-1 group was improved markedly at the 6th day and the 8th day (P<0.05). The protein level of CPT-1A in control group was not changed significantly. While it was greatly increased in the cells treated with GLP-1, which was increased 3.57 times as compared to control group at the 8th day (P<0.01). Conclusion   GLP-1 can significantly promote the expressions of FABP-4 and CPT-1A during the process of 3T3-L1 preadipocyte differentiation, which indicates that GLP-1 can simultaneously promote the fatty acid absorption and decomposition and it has played an important role in improving the free fatty aicd induced insulin resistance.

    Effect of fasudil on the expression of BMPR in rats with monocrotalineinduced pulmonary arterial hypertension
    WANG Chun-xiao, XIA Wei, LI Fu-hai, WU Zhi-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  28-33. 
    Abstract ( 1192 )   PDF (2693KB) ( 352 )   Save
    Related Articles | Metrics

    Objective   To explore the mechanism of fasudil improving MCT-induced PAH by studying the effect of fasudil on the expression of bone morphogenetic protein receptor (BMPR) in rat of monocrotaline (MCT)-induced pulmonary arterial hypertension. Methods   A total of 60 healthy Wistar rats of 4 weeks old were randomly divided into 6 groups: the early normal control group, the early model control group, the early fasudil group, the later normal control group, the later model control group, the later fasudil group. The ratios of arteriole wall thickness to vascular external diameter (WT%), and vascular area to total vascular area (WA%) were measured by a computerized image analyzer. The gene expression of BMPR1A and BMPR2 was determined by SYBGREEN RT-PCR. The protein expression of BMPR1A, BMPR2 and phosphorylated myosin phosphatase target protein-1(p-MYPT1)was determined by Western blotting. Results   Compared with the early/later normal control groups, the WT% and WA% increased significantly in the early/later model control groups(P<0.05). After treated with fasudil, the WT% and WA% in the early/later model control groups was lower than that of the early/later normal control groups(P<0.05). And in the later model control groups, the WT% and WA% obviously decreased compared with that of the early model control groups(P<0.05). The gene and protein expression of BMPR1A, BMPR2 were lower in the early/later model control groups than that in the early/later normal control groups revealed by PCR and Western blotting(P<0.05). After the treatment with fasudil, the WT% and WA% in the early/later fasudil groups increased significantly(P<0.05). The expression of p-MYPT1 makeredly elevated in the early/later model control groups compared to that in the early/later normal control groups(P<0.05). After treated wih fasudil, it decreased in the early/later fasudil groups(P<0.05). Conclusion   Fasudil can inhibit and reverse the pulmonary artery smooth muscle proliferation of MCT-induced pulmonary artery remodeling in PAH in rats effectively by upregulating mRNA and protein expressions of BMPR.

    Construction of the eukaryotic expression vectors of USF2 gene and its truncated genes and their influence on Smurf1/2 transcriptional level
    TAN Ya-wen, GAO Hai-dong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  34-37. 
    Abstract ( 1070 )   PDF (1555KB) ( 536 )   Save
    Related Articles | Metrics

    Objective    To construct the eukaryotic expression vectors of Upstream Stimulatory Factor 2 (USF2) and its truncated genes,and identify the functional region of USF2 protein down regulating the Smurf1/2 transcriptional level. Methods   Using polymerase chain reaction and gene recombination techniques,USF2 gene and its two truncated genes USF2(1-235aa) and USF2(236-346aa) were amplified and cloned into the pCMV-Myc vector respectively, and then transfected into MCF7 cells to detect their expressions. The 236-346aa region at the C terminal of USF2 inhibiting the Smurf1/2 transcription was explored by Western blot and real-time PCR techniques. Results   USF2 and its truncated genes were successfully cloned into the pCMV-Myc vector. Through Western blot and real-time PCR, the 236-346aa region at the C terminal of USF2 inhibiting the transcriptional level of Smurf1/2 was proved. Conclusion   USF2 suppresses the Smurf1/2 transcriptional level through the 236-346aa region at the C terminal.

    T541 in Q-loop of STEP plays a key role in the catalytical activity
    XIE Di-dong1,2*, GONG Zheng3*, LI Rong2, LI Hui2, LIU Hong-da2, SUN Jin-peng1,2, PANG Qi1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  38-44. 
    Abstract ( 1330 )   PDF (2375KB) ( 489 )   Save
    Related Articles | Metrics

    Objective   To explore the essential role of Threonine at position 541 and 330(T541,T330)in the intrinsic phosphatase activity of striatal-enriched protein tyrosine phosphatase(STEP). Methods   STEP wild type(STEP-WT) and its mutants STEP-T330D/T541A were sub-cloned into the PET15b vector. Expression and purification of STEP-WT and its mutants were performed by affinity column and liquid chromatography. The phosphatase activity was measured in vitro with 4nitrophenyl phosphate (pNPP) as substrate. The inhibition by NaVO3 was measured to monitor the effects of mutants on protein folding. The pH-dependence and leaving-group pKa dependence of STEP catalysis were carried out to dissect the underlying molecular mechanism. Results   STEP-WT and STEP-T330D displayed similar catalytic ability toward pNPP at pH 7.0. The kcat of STEP-T541A decreased 3 folds compared to STEP-WT.-STEP-WT and the two mutants had similar Ki for NaVO3. Examination of the kcat versus pH curve revealed that pK2app of STEP-T541A significantly increased and the (kcat)lim dropped by at least 10 folds. In consisitent with these observations, β1g(kcat) of STEP-T541A increased significantly. Conclusion   T541 plays an important role in STEP catalysis, by participating the processes from product formation to phosphate release. Future drugs targeting to STEP for therapeutic usage could be developed through modulating T541 conformations.

    Changes of substance P and betaEndorphin in the brain of  rats with trigeminal neuralgia
    HAN Jie1, LIU Yi-qing1, REN Yan-yan2, LUAN De-heng1, GAO Kun1, XIE Feng-yang1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  45-48. 
    Abstract ( 1046 )   PDF (909KB) ( 546 )   Save
    Related Articles | Metrics

    Objective   To explore the pathogenesis of trigeminal neuralgia by establishing a rat model of trigeminal neuralgia and measuring changes of SP and β-EP in hypothalamus and hypophysis. Methods   Twenty-eight male adult Sprague-Dawley rats were randomly assigned to the experimental group(Group A), the Sodium Chloride group(Group B) and the control group(Group C). In Group A, the rats were injected with 3μL penicillin G-K into the subarchnoid space caudal to obex. After the models were established, they were killed, and then their hypothalamus and hypophysis were taken out and were determined for the concentrations of SP and β-EP by radioimmunological assay(RIA) . As to Group B, the progress was the same as Group A except that the rats were injected with 3μL Sodium Chloride and were killed 30 minutes later. And the rats in Group C were directly killed, others just following Group A. Results   The concentration of SP in the experimental group was significantly higher than that in the Sodium Chloride group and the control group (P<0.01),while the concentration of β-EP was much lower(P<0.01) but there was no considerable difference between the Sodium Chloride group and the control group (P>0.05).  Conclusion   Neuropeptides, such as SP and β-EP, may play important roles in the pathphysiological mechanism of trigeminal neuralgia.

    Apoptosis-inducing factor and PARP-1 involving in gentamicin-induced vestibular hair cell death
    ZHANG Dao-gong1, FAN Zhao-min1, HAN Yue-chen1, LIU Wen-wen2,3, BAI Xiao-hui2,3, LI Ya-wei1, XU Lei1, WANG Ming-ming1, LUO Jian-fen1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  49-52. 
    Abstract ( 1110 )   PDF (2439KB) ( 453 )   Save
    Related Articles | Metrics

    Objective   To investigate the possible involvement of AIF and PARP-1 in gentamicin-induced vestibular hair cell death. Methods   Vestibular organ obtained from postnatal day (p) p3 or p4 rats were maintained in tissue culture medium and were exposed to 2 mg/mL gentamicin (GM) for up to 72 h. Apoptosis was determined by flow cytometry (FCW). Expressions of AIF and PARP-1 mRNA were determined by RT-PCR. Expressions of the mitochondrial protein and cytoplasm protein of AIF were detected by Western blotting. The expression of PARP-1 protein was detected by Western blotting. Results   GM induced apoptosis in vestibular hair cells. The number of apoptotic cells increased as shown by FCW. RT-PCR showed an up-regulation of AIF and PARP-1 mRNA in the GM group. Western blotting results showed that mitochondrial protein of AIF was decreased after gentamicin exposure while cytoplasm protein of AIF was increased. Expression of protein of PARP-1 was increased after gentamicin exposure. Conclusion   AIF participates in gentamicin-induced apoptosis of vestibular hair cells. PARP-1 might participate in the action of AIF pathway.

    Phantom investigation of the factors affecting the intensity of the twinkling artifact
    WANG Meng1, LIU Yan2, LI Jie2, SHI Dan-dan2, LIU Cong-cong2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  53-57. 
    Abstract ( 853 )   PDF (1537KB) ( 491 )   Save
    Related Articles | Metrics

    Objective   To explore the different physical factors affecting the intensity of the twinkling artifact in order to guide its clinical use. Methods   The sandpapers were selected as the phantom to qualify the roughness condition and water was selected as the acoustic window to conduct color-doppler imaging ultrasonography. All the selected parameters were changed respectively according to the principle of single variable. The optimized parameter settings were determined and used in the four different kinds of the sandpapers. All the above-mentioned data were saved and analyzed by Matlab software. The two-tail t test, one factor analysis of variance (ANOVA) and curved regression analysis were used for the comparison. Results   The optimized condition for the display of the twinkling artifact were as follows: the highest color write priority and color gain, which were just below the threshold mentioneded under for color noise, a maximum wall-filter under a higher pulse repetition frequency, a focus depth setting below the sandpaper and a color box adjusted properly in the fundamental imaging mode (P<0.05). The intensity of the twinkling artifact was in direct proportion to the roughness of the sandpaper(r=0.912, P<0.001). Conclusion   The parameter settings and the roughness of the sandpaper are found to affect the intensity of the twinkling artifact. It is of great significance to grasping the mechanism of these parameters for the improvement of the twinkling artifact in clinical use and research.

    Evaluation of efficacy and safety of manidipine hydrochloride among patients with mild or moderate essential hypertension: a multicenter, randomized, doubleblind study
    MA Xiao1, WANG Yue-ming2, LI Hong-shi3, KAN Xiao-hong4,ZHAO Jing4, YUE Xin4, ZHANG Yun4, ZHONG Ming4, LI Li4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  58-61. 
    Abstract ( 1224 )   PDF (944KB) ( 1937 )   Save
    Related Articles | Metrics

    Objective   To evaluate the efficacy and safety of manidipine hydrochloride among patients with essential mild or moderate hypertension. Methods   Two hundred and forty subjects with mild or moderate hypertension were enrolled in the study and assigned to two groups (the study group and the control group) equally by a randomized, double-blind,multicenter and positive controlled study.After a one to two-week placebo run-in period, patients with essential mild or moderate hypertension were distributed randomly into two groups to receive study drug (manidipine hydrochloride 10~20mg per day) or positive controlled drug ( amlodipine besylate 5~10mg per day) respectively for 8 weeks. BP change, tolerance and adverse events between the two groups were evaluated. Results   One hundred and three patients completed trial in the study group and 103 patients in the control group.Subjects who completed the study were with good compliance,except those dropped off or were excluded.There was no significant difference for baseline characteristics between the two groups (P>0.05) including age,sex,body mass index,baseline systolic and diastolic BP,and heart rate.The reduction of diastolic and systolic BP, heart rate and seat diastolic blood pressure normalized rate between the two groups had no significant difference at the end of treatment (P>0.05). The incidence of adverse events also had no significant difference between the two groups(P>0.05). Conclusion   The antihypertensive efficacy of manidipine hydrochloride is equal to amlodipine besylate among patients with mild or moderate hypertension. Manidipine hydrochloride is safe and can be well tolerated.

    Relationship of plasma concentration of brain natriuretic peptide and lung cancer
    CAO Hong-li1, LIU Shu-ying2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  62-64. 
    Abstract ( 1311 )   PDF (1101KB) ( 1420 )   Save
    Related Articles | Metrics

    Objective   To explore the risk factors of elevated serum brain natriuretic peptide(BNP), especially the influence of lung cancer on BNP. Methods   Retrospective study was carried out with  439 patients hospitalized from January, 2002 to January, 2013. The variables were analyzed by univariate analysis, followed by multivariate Logistic regression model to assess the dependent risk factors of elevated serum BNP(≥125pg/mL). Results   The multivariate analysis showed that female, ≥58 years old, smoking, C-reactive protein >4mg/L and lung cancer were independent risk factors for the elevated serum BNP. The risk of elevated serum BNP(≥125pg/mL) in lung cancer patients was 7 times higher (OR=7.0, 95% CI=2.9~17.0,P<0.01 ) than that in the patients without lung cancer. Conclusion   Patients with lung cancer had a higher risk of elevated serum  BNP. Therefore, lung cancer should be included in the differential diagnosis with elevated serum BNP.

    Drug sensitivity of carbapenems combined with sulbactam or cefoperazone on Pseudomonas Aeruginosa
    LI Tao1, LI Yu1, WANG Wen1, ZHANG Fan2, GU Teng-zhen3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  65-68. 
    Abstract ( 1234 )   PDF (901KB) ( 468 )   Save
    Related Articles | Metrics

    Objective   To compare drug sensitivity of carbapenems combined with sulbactam or cefoperazone on Pseudomonas Aeruginosa(PA) in vitro. Methods   After the collection of  PA strains, antimicrobial susceptibility test were conducted. The PA strains were divided into sensitive group, multidrug resistant (MDR) group, extensively drug resistant (XDR) group and pan-drug resistant(PDR) group according to the results of antibiotic sensitivity test. The sensitivities of biapenem or imipenem combined with sulbactam, cefoperazone, fosfomycin, amikacin, cefperazone-sulbactam, ciprofloxacin were examined by Kirby-Bauer (K-B) disc agar diffusion method and minimal inhibitory concentration (MIC) assay,then the fractional inhibitory concentration (FIC) indexes were calculated. Results   One hundred and twenty clinical PA strains were collected, including 56 sensitive strains, 36 MDR strains, 13 XDR strains and 15 PDR strains. Biapenem showed synergistic effects with sulbactam, fosfomycin, amikacin and cefoperazone seperately. Synergistic effects were observed in the combinations of imipenem and fosfomycin, sulbactam, amikacin. The antagonistic effects were observed in the combinations of imipenem and  cefopcrazone or cefperazone-sulbactam. Conclusion   Carbapenems combined with fosfomycin,amikacin or sulbactam can be used in the treatment of resistant PA infections, but imipenem combined with cefoperazone is not recommended.

    Multiple regression analysis of influencing factors  in FibroScan detecting liver stiffness
    WANG Ai-guang1, CUI Lei2, WANG Gui-ling2, LI Jian-zhi2, ZHANG Mei-fang2, ZHAO Min2, SUN Ai-hua2, SHAN Rong2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  69-73. 
    Abstract ( 1170 )   PDF (919KB) ( 962 )   Save
    Related Articles | Metrics

    Objective   To determine the independent predictors influencing liver stiffness detection with FibroScan by multiple regression analysis.  Methods   One hundred and eighty-one inpatients and outpatients who required liver biopsy were enrolled. Liver stiffness measurement (LSM) was detected by FibroScan on the day of performing liver biopsy, and clinical information and routine biochemical tests data (±3 days) were gathered. Ten factors were chosen for the multiple regression analysis. Results   Multiple regression analysis showed that platelet(PLT), serum albumin(ALB), prothrombin activity(PTA) and body mass index(BMI) were independent predictors. ConclusionIn    FibroScan detection, PLT, ALB, PTA and BMI might be influencing factors.

    Expressions of centrosome α tubulin, P53 protein and telomerase in hepatocarcinoma and their correlations
    LI Heng1, LI Zhe1, QIAO Xiao-jun1, GAO Ai-qin2, ZHANG Nan2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  74-77. 
    Abstract ( 1021 )   PDF (29383KB) ( 224 )   Save
    Related Articles | Metrics

    Objective   To evaluate the expressions of centrosome α tubulin, P53 protein and telomerase in human hepatocellular carcinoma (HCC), paracancerous tissues, and cirrhosis / chronic hepatitis tissues, in order to investigate the correlations between centrosome abnormalities and telomerase in hepatocellular canceration. Methods   The paraffin-embedded tissues of 47 cases of HCC, 47 paracancerous tissues and 12 cirrhosis / chronic hepatitis tissues prepared in the Pathological Department of Jinan Central Hospital were selected. Immunohistochemistry SP method was applied to detect the expressions of centrosome α tubulin, P53 protein and human telomerase reverse transcriptase (hTERT). The correlations were then analyzed. Results   The expressions of α tubulin in HCC, paracancerous and cirrhosis /chronic hepatitis tissues were 59.57%, 23.4% and 16.67%; the expressions of P53 protein were 76.60%, 29.97% and 33.33%. The expressions were significantly higher in HCC than in paracancerous and cirrhosis /chronic hepatitis tissues (P<0.05). The positive expressions of hTERT were 68.09% in HCC, 34.04% in paracancerous tissues and 0% in cirrhosis /chronic hepatitis tissues, with significant difference (P<0.05). Expressions of α tubulin, P53 protein and telomerase were positively correlated. Conclusion    Centrosome abnormalities may participate in the development of HCC, and may serve as a new target for diagnosis and therapy.

    Value of virtual touch tissue quantification in the diagnosis of chronic allograft nephropathy after renal transplantation
    LIU Yan, LIU Cong-cong, SHI Dan-dan, SUN Xiao, LI Jie
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  78-81. 
    Abstract ( 1087 )   PDF (1444KB) ( 438 )   Save
    Related Articles | Metrics

    Objective   To investigate the significance of virtual touch tissue quantification (VTQ) for the assessment of chronic allograft nephropathy (CAN). Methods   A total of 28 patients with CAN were enrolled in the case group, while 33 patients with normal renal function served as the controls. All cases and controls underwent color Doppler sonography and VTQ examination. The results were compared between the two groups. Results   The mean shear wave velocity (SWV) of the cases and controls were (3.03±0.23) m/s and (2.71±0.23) m/s respectively, with statistically significant difference (P<0.05). The receiver operator characteristic (ROC) curve displayed that VTQ value of 2.83m/s could be used to diagnose CAN; the sensitivity, specificity, positive predictive value and negative predictive value were 92.9%, 66.7%, 70.3% and 91.7%, respectively. The diagnostic accuracy was 78.7%. Conclusion   Parenchymal stiffness can be quantitatively measured by VTQ, which can monitor renal function and offer more diagnostic information than conventional ultrasound examination.

    Evaluation of the new method for ultrasound measurement of fetal lateral cerebral ventricle
    LI Yang1, TAO Guo-wei2, LIU Shao-ping2, MA Zhe2, ZHI Rui-juan2, WANG Yin2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  82-84. 
    Abstract ( 1064 )   PDF (1463KB) ( 1442 )   Save
    Related Articles | Metrics

    Objective   To evaluate a new method —the Guibaud method for ultrasound measurement of the fetal lateral cerebral ventricle. Methods   One hundred and forty  fetuses’ lateral ventricles width were  measured by the routine method and the Guibaud method. The Bland-Altman statistical method was used to compare the consistency between the two methods.30 fetuses were randomly selected to evaluate the repeatability and reproducibility. Results   The Guibaud method had high consistency with the routine method. 98.6% (138/140) of the points were in the 95% limits of consistency (-0.46,0.73);the mean difference was 0.13cm and the maximum absolute value was 0.7cm.  The repeatability and reproducibility of the Guibaud method was better(P>0.05). Conclusion   The Guibaud method has high consistency with the routine method.The standard planar is simple and stable to obtain. The repeatability and the reproducibility is better. All of these improve the accuracy of the measurement. 

    Structural autograft for acetabular reconstruction in total hip arthroplasty of developmental hip dysplasia
    REN Chong-min, ZHANG Yuan-kai, LI De-qiang, LIU Pei-lai, LI Ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  85-88. 
    Abstract ( 1054 )   PDF (1218KB) ( 359 )   Save
    Related Articles | Metrics

    Objective   To evaluate the result of bulk femoral head autograft for acetabular reconstruction in total hip arthroplasty for developmental hip dysplasia patients with severe acetabular deficiency.  Methods   Twenty-five -patients(31 hips) with development hip dysplasia were performed with bulk femoral head autograft for the acetabular reconstruction in THA during 2005-2010. Twenty-nine  hips in 23 patients were successfully followed up in average 5.1 years with mean age of 52. According to Crowe classification, 19 hips in 15 patients were stage III and 10 hips in 8 patients were stage IV. The deficient acetabula were reconstructed by bulk femoral head autograft. With bone guaranteed coverage, patients had prosthesis installed at the original level or closed level of true acetabulum. Radiographic changes and Harris score were evaluated.  Results   None suffered from loosening and collapse during the follow-up. One was revised for dislocation. Harris scores increased from 44 preoperative to 88 at last follow-up.  Conclusion   To reconstruct acetabulum at true acetabular level with satisfactory bone coverage by using bulk femoral head autograft is  an effective and safe way for THA in developmental hip dysplasia.

    Vertical semicircular canal function in Meniere′s disease
    ZHU Rong-yu, SUN Qing, PENG Xin, LI Na, SHAN Xi-zheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  89-94. 
    Abstract ( 1499 )   PDF (2350KB) ( 765 )   Save
    Related Articles | Metrics

    Objective   To assess the function of vertical semicircular canal (VSCC) in patients with Meniere′s disease(MD)  and to analyze the relationship of the VSCC function and HSCC in MD. Methods   Sixty-six patients presenting with MD and 45 normal subjects were examined the VSCC function using the 180-degree vertical plane non-damping swing method with the developed automatic benign paroxysmal positional vertigo (BPPV) diagnosis and therapy system (SRM-Ⅳ). The course including the eye nystagmus orientation and the slow phase velocity(SPV) and other parameters were recorded with computers and videos. The vertical plane swing test was done in all subjects, calculating the average slow phase velocity of the vertical nystagmus and the canal paresis (CP) of the right anterior semicircular canal(SCC) -left posterior SCC plane(RALP) as well as the left anterior SCC-right posterior SCC plane(LARP). PC and SPSS17.0 software were used for statistical analysis. At the same time, all patients were examined the HSCC function with a caloric test. Results   ① Vertical nystagmus could be induced in 128 of 132 (96.97%) pairs in 66 MD patients while the CP range of the two pairs of VSCCs was 0.13%89.00%. 39 patients with MD had at least one pair of VSCCs CP≥20%. The average slow phase velocity(ASPV) of normal subjects was larger than that of patients with MD, and there was statistically significant difference in either LARP or RALP plane. ② CP of 180-degree vertical plane non-damping swing was consistent with caloric test in 63.64%. Conclusion   As supplement of the caloric test,180-degree vertical plane non-damping swing method may prove helpful in assessment of semicircular canal function.

    Determination of acipimox in human plasma by HPLC and its pharmacokinetics
    LI Rong, HUANG Jing, BU Fan-long, YUAN Gui-yan, WEI Chun-min, ZHANG Rui, WANG Ben-jie, GUO Rui-chen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  95-98. 
    Abstract ( 1079 )   PDF (1716KB) ( 700 )   Save
    Related Articles | Metrics

    Objective   To establish a quantified analytical method for the determination of acipimox in human plasma by HPLC-UV with caffeine as the internal standard. Methods   Acipimox and caffeine in plasma were separated on a C18 reversed phase column after simple protein precipitation, eluted with mobile phase of acetonitrile-2 mmol/L tetrabutyl ammonium bromide solution (pH=3, 13∶87, V/V) at a flow rate of 1.0 mL/min, and detected at 263 nm. Results   The calibration curve was linear (r2>0.99) over the concentration range of 0.05~6μg/mL. The specificity, recovery, sensitivity, linearity, accuracy, precision, and stabilities were validated for acipimox in human plasma. Conclusion   The established method is sensitive, specific and can successfully fulfill the requirements of acipimox pharmacokinetic studies.

    Association of the marital quality and psychological stress in patients with epilepsy
    DING Juan, JIANG Hong, PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  99-102. 
    Abstract ( 877 )   PDF (940KB) ( 432 )   Save
    Related Articles | Metrics

    Objective   To explore the relationship between the marital quality and psychological stress in patients with epilepsy. Methods   In the paired-case control study, 40 adult married epilepsy out-patients and 40 normal controls were investigated with Enrich Marital Inventory, self-rating depression scale (SDS), self-rating anxiety scale (SAS), and psycho-social stress scale (PSS) to analyze the marital quality and the psychological stress of the two groups. Results   The marital satisfaction, personality issues, conflict resolution, financial management, and leisure activities of the patients scored significantly lower than those of the controls (P<0.05). The scores of SAS, SDS, stress emotion reaction (SER), stress physical reaction (SPR) and negative coping (NC) were significantly higher in patients than in controls (P<0.01). The scores of positive coping (PC) was significantly lower in patients than in controls (P<0.01). The total scores of marital quality were negatively correlated with epileptic seizure type, total SDS and SAS scores, while positively correlated with the scores of PC and social support. In addition, regression analysis showed that three factors were involved, including depression (β=-1.324), epileptic seizure type (β=-31.771) and family support (β=5.192). Conclusion   The marital quality of patients with epilepsy is lower than that of the controls. The patients suffer significant anxiety, depression and stress, whose marital quality is closely related with epileptic seizure type, anxiety, depression, social support and coping style.     

    Relationship between personality traits and childhood trauma in patients with schizophrenia
    WANG Yan1, YANG Li-min1,2, SUN Meng-meng2, CUI Kai-yan2, YANG Xiao-dong2, QIAO Dong-dong1,2, WANG Ru-zhan2, ZHANG Jing-xuan2, LIU Lan-fen1,
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  103-106. 
    Abstract ( 1152 )   PDF (905KB) ( 568 )   Save
    Related Articles | Metrics

    Objective   To explore personality traits in patients with schizophrenia, and to explore its relationship with childhood trauma. Methods   Two hundred and ten  patients with schizophrenia (the case group) and 190 healthy individuals (the control group) were investigated with tridimensional personality questionnaire (TPQ) and childhood trauma questionnaire-short form (CTQ-SF). Results   The harm-avoidance dimension scores in the case group were higher compared with the control group(P<0.01); the reward-dependence dimension scores were lower in the case group than those in the control group(P<0.01). The noveltyseeking dimension scores were significantly related to emotional abuse, sexual abuse and the CTQ-SF total scores (P<0.01). The harm-avoidance dimension scores were significantly related to physical neglect scores(P<0.05). The reward-dependence dimension scores were significantly related to sexual abuse and the CTQ-SF total score (P<0.05). Multivariate stepwise regression analysis found that emotional abuse was the optimum predictor for novelty-seeking dimension and CTQ -SF total score was the optimum predictor for reward-dependence dimension. Conclusion   Patients with schizophrenia have unique characteristics in TPQ′s harm-avoidance dimension and reward-dependence dimension, and childhood trauma may influence their personality characteristics.

    Effects of vitamin E and zinc on the decreases of immunity due to phone calls in pregnant and fetal rats
    YIN Long-long, YIN Mei-shang, ZHANG Yi, AN Kang, GUO Dong-mei, CUI Xi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(8):  107-110. 
    Abstract ( 1046 )   PDF (905KB) ( 461 )   Save
    Related Articles | Metrics

    Objective   To investigate protective effects of vitamin E and zinc supplement on the influence by a certain time phone calls in pregnant rats and offspring. Methods   Sixty pregnant rats were randomly divided into six groups. All groups were exposed to a certain intensity of cell phone radiation for 1 hour each time,3 times per day for 21 days from the first day of pregnancy. The negative control group and positive control group were given vitamin E removed peanut oil , whereas the low, middle and high dose groups were administered 5,15 and 30mg/mL vitamin E peanut oil, respectively. Combined intervention group was supplemented with 15mg/mL vitamin E peanut oil and 10mg/mL sulfuric acid zinc. C3, C4, IgG, IgA and IgM levels in maternal and offspring’s serum were determined after the delivery. Results   In the positive control group, the levels of C3 and IgG in the dams and offspring rats and of C4 in the parent rats significantly reduced when compared to the negative controls (P<0.05). Compared with the positive group the C3, C4 and IgG levels in maternal and the C3, IgG levels in offspring rats increased significantly in vitamin E high dose and vitamin E+Zn combined group (P<0.05). The level of C3 in vitamin E middle dose group of mothers was also increased significantly (P<0.05). The IgG levels of offspring rats in middle, high dosages groups all increased and the differences were significant when compare to the control croup (P<0.05). The differences of C3 levels in the offspring rats and the C4 levels in maternal rats between combination group and middle dose group were significant (P<0.05). Conclusion   Vitamin E has a protective effect against damage to C3, C4, IgG induced by a certain time phone calls in pregnant mice and neonatal rat. Vitamin E combined with zinc has synergistic effect.