Abstract:

Objective   To establish a quantified analytical method for the determination of acipimox in human plasma by HPLC-UV with caffeine as the internal standard. Methods   Acipimox and caffeine in plasma were separated on a C18 reversed phase column after simple protein precipitation, eluted with mobile phase of acetonitrile-2 mmol/L tetrabutyl ammonium bromide solution (pH=3, 13∶87, V/V) at a flow rate of 1.0 mL/min, and detected at 263 nm. Results   The calibration curve was linear (r2>0.99) over the concentration range of 0.05~6μg/mL. The specificity, recovery, sensitivity, linearity, accuracy, precision, and stabilities were validated for acipimox in human plasma. Conclusion   The established method is sensitive, specific and can successfully fulfill the requirements of acipimox pharmacokinetic studies.

Key words: Acipimox; Plasma; Chromatography; High performance liquid; Pharmacokinetics