JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2013, Vol. 51 ›› Issue (9): 22-25.

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Effect and expression of GRK2 on collagen synthesis in myocardial fibrosis rat model

KAN Li-li1, JIANG Fang-guo1, AN Feng-shuang2   

  1. 1. Department of Intensive Care Unit, General Hospital of Yankuang Group Co. Ltd, Zoucheng 273500, Shandong, China;
    2. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2013-05-15 Online:2013-09-10 Published:2013-09-10

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Abstract:

Objective  To study the role of G protein-coupled receptor kinase 2 (GRK2) on myocardial fibrosis. Methods    Rat model of myocardial fibrosis was established by subcutaneous injection with isoproterenol. Myocardial histological changes of the rats were tested by microscope after hematoxylin and eosin staining (HE). Myocardial fibroblasts were isolated and cultured with differential anchoring velocity. The expressions of GRK2 and collagen type Iα were examined by western blotting. The cAMP level was tested after GRK2 gene was silenced by siRNA. Results   HE staining showed myocardium necrosis, hypertrophy, and lymphocytic infiltration. The expression of GRK2 (P＜0.05) and collagen type Iα (P＜0.05) were up-regulated in isoproterenol-induced rats. The level of cAMP decreased significantly in isoproterenol-induced rats (P＜0.05). However, the level of cAMP increased after GRK2 gene silenced by siRNA (P＜0.05). Conclusion    The intracellular cAMP   increased after GRK2 gene expression is silenced by siRNA. GRK2 may serve as a novel potential therapeutic target.

Key words: Myocardial fibrosis; G-protein-coupled receptor kinase 2; Cyclic adenosine monophosphate

CLC Number: 

  • R542.2
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