Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (10): 79-85.doi: 10.6040/j.issn.1671-7554.0.2017.985

Previous Articles    

Effects of TAZ knockdown on the proliferation and apoptosis of TSCC cell line CAL27 and the molecular mechanism

CHEN Xiyan1,2, WANG Qi1,2, GU Weiting3, WEN Yong1,2   

  1. 1. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, Shandong, China;
    2. School of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    3. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2017-10-17 Published:2022-09-27

PDF (PC)

106

Abstract: Objective To explore the effects of TAZ knockdown on the proliferation and apoptosis of tongue squamous cell carcinoma(TSCC)cell line CAL27, and the possible molecular mechanism. Methods CAL27 cells were divided into the interference group and control group. The former was transfected with targeting TAZ shRNA, and the latter with non-specific targeting sequence. The interference efficiency was detected with real-time fluorescent quantitative PCR(RT-PCR)and Western blotting. The changes of proliferation were detected with CCK-8 assay and EdU staining assay. The cell cycle distribution and cell apoptosis were tested with flowcytometry. The expressions of proteins related to cell cycle and apoptosis were determined with Western blotting. Results The results of RT-PCR and Western blotting showed 山 东 大 学 学 报 (医 学 版)56卷10期 -陈希彦,等.干扰TAZ基因对舌鳞癌细胞CAL27增殖凋亡的影响及其机制 \=-that the mRNA and protein expressions of the interference group decreased sufficiently. The CCK-8 and EdU staining assay indicated that knockdown of TAZ led to the inhibition of proliferation activity. Flowcytometry results showed that cell cycle arrested in G0/G1 phase and cell apoptosis increased after TAZ knockdown. Meanwhile, knockdown of TAZ decreased the expressions of CYCLIN D1, CDK4, and CDK6. By contrast, the expressions of proapoptotic proteins like Bax and cleaved Caspase9 increased, while the expression of anti-apoptotic protein like Bcl-2 decreased. Conclusion Knockdown of TAZ can affect the proliferation and apoptosis of TSCC cell line CAL27 by modulating the expression of proteins related to cell cycle and apoptosis.

Key words: TAZ gene, Tongue squamous cell carcinoma CAL27 cell line, Cell proliferation, Cell apoptosis

CLC Number: 

  • R739.8
[1] 杨凯, 温玉明, 李龙江, 等. 舌鳞状细胞癌预后因素的探讨[J]. 华西口腔医学杂志, 2001, 19(3): 161-163. YANG Kai, WEN Yuming, LI Longjiang, et al. Investigation of the tongue squamous cell carcinoma prognostic factors[J]. West China Joural of Stomatology, 2001, 19(3): 161-163.
[2] 郑家伟, 李金忠, 钟来平, 等. 口腔鳞状细胞癌临床流行病学研究现状[J]. 中国口腔颌面外科杂志, 2007, 5(2): 83-90. ZHENG Jiawei, LI Jinzhong, ZHONG Laiping, et al. Clinical epidemiology and risk factors of oral squamous cell carcinoma: An overview[J]. China Journal of Oral and Maxillofacial Surgery, 2007, 5(2): 83-90.
[3] Chaturvedi AK, Anderson WF, Lortet-Tieulent J, et al. Worldwide trends in incidence rates for oral cavity and oropharyngeal cancers[J]. J Clin Oncol, 2013, 31(36): 4550-4559.
[4] Chi AC, Day TA, Neville BW. Oral cavity and oropharyngeal squamous cell carcinoma-an update[J]. CA Cancer J Clin, 2015, 65(5): 401-421.
[5] Zhao B, Tumaneng K, Guan KL. The Hippo pathway in organ size control, tissue regeneration and stem cell self-renewal[J]. Nat Cell Biol, 2011, 13(8): 877-883.
[6] Harvey KF. Growth control: re-examining Zyxin's role in the hippo pathway[J]. Curr Biol, 2015, 25(6): R230-R231.
[7] Harvey KF, Zhang X, Thomas DM. The Hippo pathway and human cancer[J]. Nat Rev Cancer, 2013, 13(4): 246-257.
[8] Wang J, Rouse C, Jasper JS, et al. ABL kinases promote breast cancer osteolytic metastasis by modulating tumor-bone interactions through TAZ and STAT5 signaling[J]. Sci Signal, 2016, 9(413): 12. doi: 10.1126/scisignal.aad3210.
[9] Xiao H, Jiang N, Zhou B, et al. TAZ regulates cell proliferation and epithelial-mesenchymal transition of human hepatocellular carcinoma[J]. Cancer Sci, 2015, 106(2): 151-159.
[10] Buglioni S, Vici P, Sergi D, et al. Analysis of the hippo transducers TAZ and YAP in cervical cancer and its microenvironment[J]. Oncoimmunology, 2016, 5(6): e1160187. doi: 10.1080/2162402X.2016.1160187.
[11] Li YW, Shen H, Frangou C, et al. Characterization of TAZ domains important for the induction of breast cancer stem cell properties and tumorigenesis[J]. Cell Cycle, 2015, 14(1): 146-156.
[12] Hiemer SE, Zhang L, Kartha VK, et al. A YAP/TAZ-regulated molecular signatureis associated with oral squamous cell carcinoma[J]. Mol Cancer Res, 2015, 13(6): 957-968.
[13] Jotte RM, Spigel DR. Advances in molecular-based personalized non-small-cell lung cancer therapy: targeting epidermal growth factor receptor and mechanisms of resistance[J]. Cancer Med, 2015, 4(11): 1621-1632.
[14] Rosell R, Carcereny E, Gervais R, et al. Erlotinib versus standard chemotherapy as fi rst-line treatment for European patients with advanced EGFR mutation-positive non-small-cell lung cancer(EURTAC): a multicentre, open-label, randomised phase 3 trial[J]. Lancet Oncol, 2012, 13(3): 239.
[15] Edgar BA. From cell structure to transcription: Hippo forges a new path[J]. Cell, 2006, 124(2): 267-273.
[16] Xie D, Cui J, Xia T, et al. Hippo transducer TAZ promotes epithelial mesenchymal transition and supports pancreatic cancer progression[J]. Oncotarget, 2015, 6(34): 35949-35963.
[17] Di Benedetto A, Mottolese M, Sperati F, et al. Association between AXL, Hippo Transducers, and survival outcomes in male breast cancer[J]. J Cell Physiol, 2017, 232(8): 2246-2252.
[18] Li Z, Wang Y, Zhu Y, et al. The Hippo transducer TAZ promotes epithelial to mesenchymal transition and cancer stem cell maintenance in oral cancer[J]. Mol Oncol, 2015, 9(6): 1091-1105.
[19] Gandhirajan RK, Jain M, Walla B, et al. Cysteine S-glutathionylation promotes ptability and activation of the Hippo downstream effector transcriptional co-activator with PDZ-binding motif(TAZ)[J]. J Biol Chem, 2016, 291(22): 11596-11607.
[20] 袁丽, 郭硕, 曹颖, 等. Bcl-2基因多态性与食管癌及癌前病变发病风险的相关性[J]. 肿瘤, 2015, 35(12): 1322-1328. YUAN Li, GUO Shuo, CAO Ying, et al. Association between Bcl-2 gene polymorphism and risk of esophageal squamous cell carcinoma and precancerous lesions[J]. Tumor, 2015, 35(12):1322-1328.
[21] 郑虹, 耿明. HSP70、Caspase3在胶质细胞瘤中的表达及与肿瘤病理分级的关系[J]. 山东大学学报(医学版), 2007, 45(9): 944-946. ZHENG Hong, GENG Ming. Expression of HSP70 and Caspase3 in human glioma and its relationship with pathological grades[J]. Journal of Shandong University(Health Science), 2007, 45(9): 944-946.
[22] Ruijtenberg S, van den Heuvel S. Coordinating cell proliferation and differentiation: Antagonism between cell cycle regulators and cell type-specific gene expression[J]. Cell Cycle, 2016, 15(2): 196-212.
[23] Shyam N, Rao NN, Narang RD, et al. Immunohistochemical characterization of cyclin dependent kinase-4 in different histological grades of oral leukoplakia and oral squamous cell carcinoma[J]. J Oral Maxillofac Pathol, 2014, 18(1): 36-41.
[24] Wang X, Freire Valls A, Schermann G, et al. YAP/TAZ Orchestrate VEGF Signaling during Developmental Angiogenesis[J]. Dev Cell, 2017, 42(5): 462-478.
[1] ZHAO Ge, ZOU Cunhua, SONG Dongdong, ZHAO Shuping. Effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 53-58.
[2] REN Hong, WANG Wei, LIN Yan, LOU Jianwei, JI Kunqian, ZHAO Yuying, YAN Chuanzhu. A case report of Barth syndrome [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 123-127.
[3] WANG Zhengyang, XIA Yan, SHI Kaixuan, TAO Kun, WANG Xiaojie. Effects of Trametinib on PAX8 expression in ovarian cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(10): 23-29.
[4] DU Tiantian, LI Juan, ZHAO Yinghui, DUAN Weili, WANG Jing, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression profiles of long non-coding RNA LINC02474 and effects on cell proliferation in colorectal cancer [J]. Journal of Shandong University (Health Sciences), 2021, 59(10): 57-67.
[5] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32-37.
[6] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7-14.
[7] LI Xiaofeng, DU Xiaoyi, LIU Hainan, LIU Cheng, FAN Yidong. Berberines effects on the proliferation, apoptosis and DNA damage and repair of human renal cell carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 54-59.
[8] HUANG Ping, ZHANG Kun, LI Fang, HUANG Guobao, YAN Bing,XIAO Dongjie, WANG Yunshan, LIU Hua. Comparative study on biological characteristics of mesenchymal stem cells from human umbilical cord and adipose [J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 72-78.
[9] YANG Feilong, ZHOU Zunlin, REN Juchao, YAN Lei, LIU Hainan, ZHANG Wenhua, YU Nengwang, LI Dawei, XU Zhonghua. Effects and mechanisms of hepatoma-derived growth factor on the proliferation of prostate cancer cells [J]. Journal of Shandong University (Health Sciences), 2018, 56(1): 62-69.
[10] LI Hongzhi, LIU Jing, SONG Yan, CHI Lingyi, LIU Yuguang. Role of liraglutide in the repair of spinal cord injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 1-5.
[11] YOU Jiebing, SUN Zhongwen, DU Juan, XU Wenjuan, WANG Yalin, LI Shuai, ZHU Meijia. Effects of rosiglitazone and insulin on the expression of TIPE2 and cell apoptosis in the brain of type 2 diabetic GK rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 43-48.
[12] YANG Lu, LIU Yanguo, LI Jisheng, WANG Xiuwen. Effect of bufalin on chemosensitivity to cisplatin in non-small cell lung cancer and the underlying mechanisms [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 6-11.
[13] TU Yan, LI Zhenjiang, TANG Aiping, FEI Yan, LI Huihui, LI Jian, HE Wenfeng. RNAi-mediated silencing of CD147 inhibits the proliferation and invasion of leukemic cells SHI-1 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 1-5.
[14] DONG Ke1, PU Yedi2, LIU Qiong1, DAI Guangxia1, LI Lizhen1, LI Hao1, WANG Lingling1, WANG Luqun1. Inhibition of proliferation of human non-Hodgkins lymphoma Raji cells by small interference RNA silencing Pokemon gene [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 53-57.
[15] XU Min, ZHANG Xianghua, SUN Aili, NI Yihong, SUN Fudun, CHEN Shihong. High glucose induced RSC96 Schwann cells injury in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 44-48.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd