JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (1): 49-53.doi: 10.6040/j.issn.1671-7554.0.2016.1548

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Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line

ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia   

  1. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-11-22 Online:2017-01-10 Published:2017-01-10

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Abstract: Objective To detect the expression of liver X receptor α(LXRα)in normal ovarian tissues and ovarian cancer tissues, and to investigate the effects of the LXRs agonist T0901317 on SKOV3 cell line. Methods A total of 51 cases of ovarian cancer treated in our hospital during Aug. 2015 and Aug. 2016 were enrolled. Another 32 cases of benign ovarian lesions served as controls. The expression of LXRα was detected with immunohistochemistry. The proliferation of SKOV3 cell line treated with T0901317 was detected with CCK8 assay. The effect of T0901317 on the apoptosis of SKOV3 cell line was assayed with flow cytometry. Results There was significant difference in LXRα expression between normal ovarian tissues and ovarian cancer tissues(P<0.001). T0901317 significantly inhibited the proliferation of SKOV3 cell line in a dose- and time- dependent manner(P<0.05). Activation of LXRs could induce the apoptosis of SKOV3 cell line significantly(P<0.05). Conclusion The down-regulation of LXRs receptor may be related to the pathogenesis of ovarian cancer. For advanced ovarian cancer, activating LXR pathway is therefore a potential adjuvant therapy.

Key words: Ovarian cancer, Proliferation, Liver X receptors, Apoptosis, T0901317 agonist

CLC Number: 

  • R737.31
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