JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

Previous Articles     Next Articles

Inhibitory effect and its mechanism of emodin on the proliferation of human osteosarcoma cell line

XU Liangzhi1, CHEN Kunlun2   

  1. 1. Department of Orthopaedics, Nuclear Industry 417 Hospital, Lintong 710600, Shaanxi, China;
    2. Department of General Surgery, the Second Affiliated Hospital, School of Medicine,
    Xian Jiaotong University, Xi'an 710004, Shaanxi, China
  • Received:2013-12-02 Online:2014-06-10 Published:2014-06-10

PDF (PC)

165

Abstract: Objective  To explore the inhibitory effect of emodin on the proliferation of human osteosarcoma cells SOSP9607 and the related mechanisms. Methods  Human osteosarcoma cells SOSP9607 were divided randomly into control group and treatment groups (emodin: 25, 50 and 100μmol/L). The growth inhibition ratio (IR) of emodin on osteosarcoma cells was detetced with MTT assay. The apoptosis was analyzed with flow cytometry. The changes of expressions of caspase-3, caspase-9, ERK1/2 and p-ERK1/2 were tested with Western blotting. Results  All of the cells in the treatment groups were obviously inhibited compared to those in control group, which was dose-dependent. Compared with control group, the apoptosis ratio of treatment group increased. Western blotting showed that the expressions of caspase-3 and caspase-9 protein increased in treatment group compared to control group, while the expression of p-ERK1/2 protein decreased. Conclusion  Emodin could inhibit the proliferation of human osteosarcoma cell by suppressing ERK signaling pathway and activating caspase-3 and caspase-9.

Key words: Emodin, ERK1/2, Apoptosis, Osteosarcoma

CLC Number: 

  • R738.1
[1] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[2] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32-37.
[3] ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong. An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 13-17.
[4] LI Rui, MA Weihong, REN Manyi, ZHAO Mengmeng, JIANG Shan, JU Yuanyuan, GUO Ying, SUN Zhaohui, SUI Shujian. Correlation between TWEAK and cardiac remodeling in essential hypertensive patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 49-55.
[5] GUO Hehe, SUN Zhiqiang, LIU Yanjuan, LIU Yichen, LI Guang, ZHENG Fang. Norcantharidin influences the expression of Notch signal pathway in multiple myeloma U226 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 32-37.
[6] TU Yunhua, CHEN Liyuan, WANG Xi, ZHOU Ying, KANG Yingqian, XUE Yuecui, RONG Dongyun, YE Zhenyuan, CAO Yu. Effect of ar-turmerone derivatives on proliferation and apoptosis in human melanoma WM35 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(2): 68-73.
[7] GONG Luwei, ZHOU Lizhen, SU Guohai. Perindopril ameliorates cardiac dysfunction via Akt-FoxO1 pathway in diabetic cardiomyopathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 65-70.
[8] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[9] ZHANG Kun, ZHAGN Xiaoguo, CUI Xingxing, CHEN Shijun. Expression of apoptosis-related genes in peripheral blood mononuclear cells of patients with HBV infection and the relationship with chronicity of hepatitis B [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 38-42.
[10] JU Yuanyuan, REN Manyi, LI Rui, ZHAO Mengmeng, SUI Shujian. Tumor necrosis factor-like weak inducer of apoptosis promotes expression of matrix metalloproteinase 2 and collagen Ⅰ in rat cardiac fibroblasts via the ERK1/2 signaling pathway [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(5): 23-28.
[11] LI Hongzhi, LIU Jing, SONG Yan, CHI Lingyi, LIU Yuguang. Role of liraglutide in the repair of spinal cord injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 1-5.
[12] REN Baoxin, MA Yunfeng, LIU Dianwei, LI Zhuo, JIANG Yong. Effects of Wnt3a on neuronal autophagy and apoptosis in early brain injury after subarachnoid hemorrhage in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 11-15.
[13] HAO Fengqin, LI Na. Neuroprotective effect of flavanoids extracted from onion on retinal ganglion cells with diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 7-10.
[14] CHENG Xiangyu, XING Rui, XING Zhaoquan, GUO Zhaoxin, GUO Xiaoyu, SU Jing, MENG Liwei, LIU Zhaoxu. Effects of nitidine chloride on the proliferation and apoptosis of prostate cancer PC-3 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 13-18.
[15] GUO Changyan, QIN Xuejiao, WU Xiaoli, GAO Xue, CAI Keli. Protective effect of mitochondria-targeted peptide SS-31 against H2O2-induced apoptosis in human lens epithelial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 23-26.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd