Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (3): 35-40.doi: 10.6040/j.issn.1671-7554.0.2021.0176

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Periodontal ligament stem cells regulate the functions of macrophages in vitro

ZHANG Ludan1, DING Xiaoling2, CUI Shuyue1, CHENG Chen1, WEI Fulan3, DING Gang1   

  1. 1. School of Stomatology, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Clinical Competency Training Center, Weifang Medical University, Weifang 261053, Shandong, China;
    3. School of Stomatology, Shandong University, Jinan 250012, Shandong, China
  • Published:2021-04-06

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Abstract: Objective To explore the effects of periodontal ligament stem cells(PDLSCs)on the phenotypes and functions of macrophages. Methods After PDLSCs were isolated and cultured, the expression profiles of STRO-1, CD146 and CD90, as well as the multipotent differentiation capabilities were detected. After macrophages were isolated from peripheral blood, they were cocultured with an equal amount PDLSCs in Transwell co-culture condition at 37 ℃ and 5% CO2, which were set as the experimental group. Macrophages cultured alone were set as the control group. After 3d co-culture, the expression profiles of CD14+CD206+ macrophages were examined by flow cytometry. After 24 h co-culture, macrophages were obtained, fluorescein isothiocyanate labeled dextran was added. Then, after 30 min incubation, the phagocytosis rate of macrophages was detected with flow cytometry. After 3 d co-culture, the supernatant was collected, and the concentrations of IL-10, IL-6 and TNF-α were determined with enzyme-linked immunosorbent assays. Results PDLSCs displayed fusiform fibroblast-like morphology, positive for the mesenchymal stem cells surface markers including STRO-1, CD146 and CD90, and could differentiate into bone cells and lipid cells. Compared with the control group, the experimental group had significantly increased expression of CD14+CD206+macrophages ［(38.73±6.32)% vs(8.39±2.71)%, t=127.7, P=0.004 9), unchanged phagocytosis rate of macrophages ［(36.7±5.1)% vs(38.6±4.3)%, t=3.904, P=0.159 6］, elevated level of IL-10 ［(382.5±18.2)pg/mL vs(198.5±11.4)pg/mL, t=76.36, P=0.000 3］, but decreased levels of IL-6 ［(453.1±70.42)pg/mL vs(936.7±49.9)pg/mL, t=53.12, P=0.011 5)and TNF-α ［(64.9±11.3)pg/mL vs(131.7±19.3)pg/mL, t=51.48, P=0.000 6］. Conclusion PDLSCs are capable of converting macrophages into M2 phenotype without affecting the phagocytic functions. Meanwhile, they can stimulate the secretion of IL-10 but inhibit the secretion of IL-6 and TNF-α.

Key words: Periodontal ligament stem cells, Macrophages, Polarization, Transwell co-culture system

CLC Number: 

  • R781
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