JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

Previous Articles     Next Articles

Methamphetamine inhibits spontaneous action potential frequency and hyperpolarization-activated cation current in mesolimbic dopaminergic neurons

YUE Qingwei, ZHU Dexiao, WU Jintao, LIU Haili, ZHANG Jing, LI Guibao, DING Zhaoxi, SUN Jinhao   

  1. Institute of Human Anatomy, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2013-11-05 Online:2014-05-10 Published:2014-05-10

PDF (PC)

70

Abstract: Objective  To label and identify the mesolimbic dopaminergic neurons and observe the effects of high concentration methamphetamine (MA) on their electrophysiological properties and hyperpolarization-activated cation current(Ih). Methods  Fluorescent retrograde tracer was injected into nucleus accumbens (NAc) with stereotaxic apparatus. The labeled cells were identified with tyrosine hydroxylase (TH) immunohistochemical staining. Whole-cell patch-clamp recordings were prepared from the labeled neurons in brain slices with and without MA (10μmol/L) treatment. Results  Mesolimbic neurons projected to NAc were successfully labeled with fluorescence tracer. Immunohistochemical staining showed that labeled cells were TH positive, which mainly distributed in the ventral tegmental area (VTA). The spontaneous action potential frequency of labeled neurons was obviously reduced by MA treatment (P<0.05). Besides, the Ih current was obviously inhibited (P<0.05) by MA.  Conclusion  MA inhibits the spontaneous action potential frequency and Ih of mesolimbic dopaminergic neurons.

Key words: Methamphetamine, Neurons, Dopamine, Hyperpolarization-activated cation current, Rats

CLC Number: 

  • R338.8
[1] ZHU Lin, HU San-Yuan, ZHANG Guang-Yong, DING Xiang-Jiu. Protection of PGE2 against intestinal mucosa injuries in obstructed jaundice rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 12-.
[2] SUN Tao, ZHANG Dao-Lai, XIE Shan-Shan, WANG Yu-Zhuo, FENG Yu-Xin, XIN Hua. Effect of ethanol on expression of connexin 43 in primary culture of neural precursor cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 20-.
[3] ZHANG Dao-Lai, SUN Tao, XIE Shan-Shan, WANG Yu-Zhuo, ZHAO Ling, FENG Yu-Xin, XIN Hua. Developmental expression of NMDAR1 subunit on cultured cerebral cortical neurons of fetal rats in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 28-32.
[4] ZHANG Liang, XU Min, ZHUANG Xianghua, LOU Fuchen, LOU Nengjun, LÜ Li, GUO Wenjuan, ZHENG Fengjie, CHEN Shihong. An investigation of endoplasmic reticulum stress and apoptosis in diabetic peripheral neuropathy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 13-17.
[5] GOU Yun, ZHOU Bo, WEI Cao, CHEN Yunhua, XU Li, LIU Fen, ZHANG Chunlin, WEN Min. Protection of thioctic acid in mitochondria in the rat model of Parkinsons disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 18-23.
[6] XIE Haibin, WU Qunzheng, LIU Shaozhuang, HUANG Xin, CHENG Yugang, HU Sanyuan, ZHANG Guangyong. Role of mitochondria-associated membranes in the improvement of insulin sensitivity in diabetic rats after sleeve gastrectomy [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 36-40.
[7] CHEN Wukang, LÜ Cuixia, ZHANG Xiaofei, DONG Lei, DU Yihui, FU Jihua. Prevalence and influencing factors of methamphetamine use among female sex workers [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 73-76.
[8] CHEN Yihui, LIU Xuewei, HOU Xuhao, SUN Qinfeng. Study of mouse alveolar bone defect reparation by dialdehyde bletilla striata glucomanna / hydroxypropyl chitosan / nano-nacre powder composited scaffolds [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 7-11.
[9] LI Guohua, CHI Heng, XING Huimin, TANG Jiyou. Effects and potential mechanism of orexin-A on hippocampal neurons under hypoxia condition [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 17-24.
[10] LI Hongzhi, LIU Jing, SONG Yan, CHI Lingyi, LIU Yuguang. Role of liraglutide in the repair of spinal cord injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 1-5.
[11] REN Baoxin, MA Yunfeng, LIU Dianwei, LI Zhuo, JIANG Yong. Effects of Wnt3a on neuronal autophagy and apoptosis in early brain injury after subarachnoid hemorrhage in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 11-15.
[12] CHEN Zhixin, WANG Ying, CAO Xinran, HEI Naihao, LI Junlong, DONG Bo, GUAN Guangju. Evaluation of the protective effect of AVE0991 on streptozotocin-induced diabetic nephropathy rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 29-33.
[13] ZHANG Dongqing, WANG Yong, CHEN Shouzhen, ZHU Yaofeng, SHI Benkang. Changes on the expressions of alpha1-adrenergic receptor and nerve growth factor and their effects on urethral function in diabetic rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 30-34.
[14] FENG Xiaoyu, ZHANG Hongmei, CHE Xuanqiang, KANG Donghong. Therapeutic effects of human osteoblast-stimulating factor on osteoporosis of ovariectomized rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 8-12.
[15] JIANG Lei, ZHANG Lei, LIANG Jiangjiu. Expression profiles of long noncoding RNAs in rat myocardial hypertrophy induced by pressure overload [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 21-26.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd