JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (4): 17-24.doi: 10.6040/j.issn.1671-7554.0.2015.1024

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Effects and potential mechanism of orexin-A on hippocampal neurons under hypoxia condition

LI Guohua1,2, CHI Heng1, XING Huimin2, TANG Jiyou1   

  1. 1. Department of Neurology, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    2. Department of Neurology, Third Peoples Hospital of Jinan, Jinan 250132, Shandong, China
  • Received:2015-10-28 Online:2016-04-10 Published:2016-04-10

Abstract: Objective To investigate the effects of orexin-A(OXA)on the hippocampal neurons of Wistar rats in the state of oxygen glucose deprivation(OGD)and its potential mechanism. Methods Hippocampal nerve cells of Wistar rats were divided into the control group, OGD group, OGD+OXA groups(including OGD+OXA 1 nmol/L group, OGD+OXA 3 nmol/L group, OGD+OXA 10 nmol/L group and OGD+OXA 100 nmol/L group), OGD+U0126 group, and OGD+OXA+U0126 group. The hypoxia models of primary hippocampal neurons were built after 72-hour culture, and then different concentrations of OXA(1, 3, 10 and 100 nmol/L)were added in after OGD. The effects of OXA on the apoptotic rate of hippocampus neurons were detected 48 hours later. MAPK/ERK1/2 pathway was blocked by U0126 to investigate the molecular mechanism. Results Compared with OGD group, the apoptotic rates of hippocampal cells of OGD+OXA 3 nmol/L group, OGD+OXA 10 nmol/L group and OGD+OXA 100 nmol/L group were significantly increased(P<0.01); there was no significant difference between the OGD+OXA 10nmol/L group and OGD+OXA 100 nmol/L group(P>0.05), while the cell apoptotic rate of these two groups were higher than that of OGD+OXA 3 nmol/L group(P<0.01). After U0126 treatment, the apoptotic rate of hippocampal neurons of OGD+U0126 group was significantly lower than that of OGD group(P<0.01), and that of the OGD+U0126+OXA group was lower than OGD+OXA 100 nmol/L group(P<0.01). Conclusion OXA at high concentration can aggravate the damage of 山 东 大 学 学 报 (医 学 版)54卷4期 -李国华,等.Orexin-A对缺氧状态下海马神经元的影响及其机制 \=-OGD on hippocampal neurons, which closely associates with the excessively stimulated ERK1/2 signal pathway by OXA.

Key words: Extracellular signal-regulated kinase, Oxygen glucose deprivation, Hippocampal neurons, Orexin-A

CLC Number: 

  • R74
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