Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (2): 80-87.doi: 10.6040/j.issn.1671-7554.0.2017.804

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Carbon disulfide induced embryo implantation by changing the polarization and functional of ovarian corpus luteum macrophages

ZHANG Tongchao1, WANG Zhiping2   

  1. 1. Department of Epidemiology;
    2. Department of Occupational and Environmental Health, School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-28

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Abstract: Objective To investigate the polarization and functional changes of ovarian corpus luteum macrophages in pregnant mice exposed to carbon disulfide(CS2)and to explore the role of macrophages in implantation failure induced by CS2 from the perspective of immune injury. Methods The animal model of exposure to CS2 at different time points was established. Single dose of CS2(0.4 LD50, 631.4 mg/kg)was injected intraperitoneally at the third(GD3), fourth(GD4), and fifth(GD5)day of gestation, and the solvent control group was set up(equal volume of olive oil). The continuous observation endpoints were designed at different time points after exposure. Ovarian tissues were collected for HE staining, immunohistochemistry, and real-time quantitative PCR detection, and the polarization and functional changes of macrophages were also determined. Results (1) The polarization balance of ovarian corpus luteum macrophages changed from the M1 type of macrophages(M1>M2, such as GD3-treated group at GD4 observation endpoint, t=10.000, P<0.001)to the M2 type of macrophages(M1F=10.235, P=0.003). (2) The expression of TNF-α and Vegfa mRNA increased significantly(such as Vegfa in GD4- 山 东 大 学 学 报 (医 学 版)56卷2期 -张同超,等.二硫化碳通过改变卵巢黄体巨噬细胞极化和功能导致胚胎植入障碍 \=-treated group at GD7 observation endpoint, F=6.801, P=0.007). The ovarian corpus luteum mainly characterized by vasodilatation and congestion, and structural changes(such as the structure evaluation of GD4-treated group at GD5 observation endpoint, F=8.638, P=0.019). The expression of Cox2 and Hif1-α mRNA showed a tendency of initial increase and then decrease(such as Cox2 in GD3-treated group at GD5 observation endpoint, F=6.184, P=0.035). Conclusion The mechanism of embryo implantation failure induced by CS2 exposure may be due to the polarization and functional changes of ovarian corpus luteum macrophages, which triggers and aggravates the damage in the structure and function of ovarian corpus luteum.

Key words: Carbon disulfide, Macrophage polarization, Ovarian corpus luteum, Embryo implantation disorder, Mice

CLC Number: 

  • R114
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