Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (2): 34-40.doi: 10.6040/j.issn.1671-7554.0.2020.1588

Previous Articles     Next Articles

Expression and clinical significance of circulating CD56bright subset of NK cells in patients with Parkinsons disease

LI Xiangqing, YIN Xin, ZHAO Xuelian, ZHAO Peiqing   

  1. Department of Neurology, Zibo Central Hospital, Zibo 255036, Shandong, China
  • Published:2021-03-05

PDF (PC)

333

Abstract: Objective To analyze the expression and clinical significance of circulating CD56bright subset of NK cellsinpatients with Parkinsons disease(PD). Methods A total of 56 PD patients treated during Oct. 2017 and Dec. 2019 and 50 healthy controls matched in age, sex and living environment were enrolled. The levels of cytokines in the peripheral blood of PD patients were detected with ELISA; the ratios of NK cells and CD56bright subset were analyzed with flow cytometry; PD patients were stratified with Hoehn-Yahr staging scale and unified Parkinsons disease rating scale(UPDRS); the correlation between the ratio of CD56bright subset and disease progression was analyzed. Finally, CD56bright subset was selected and cultured to clarify the differences in cytokines between PD group and control group. Results The levels of cytokines in PD group were significantly higher than those in the control group［IL-1β(t=2.46, P=0.015 4), IL-6(t=3.19, P=0.001 9), TNF-α(t=3.37, P=0.001 1), IFN-γ(t=6.36, P<0.001)］. The ratio of NK cells(t=3.13, P=0.007 8)and CD56bright subset(t=4.23, P<0.001)in PD group were significantly increased. The ratio of CD56bright subset was positively correlated with the progression of PD［Hoehn-Yahr staging scale(t=3.79, P=0.005 2), UPDRS(t=3.08, P=0.041)］. PD group could secrete more cytokines than control group after LPS stimulation［IL-1β(t=2.89, P=0.044 6), IL-6(t=3.15, P=0.034 7),TNF-α(t=4.59, P=0.010 1), IFN-γ(t=4.79, P=0.008 7)］. Conclusion NK cells and CD56bright subset are highly expressed in PD patients. CD56bright subset can promote the progression of PD by secreting cytokines, and CD56bright subset can become a new biomarker for diagnosis and a new target for predicting PD prognosis.

Key words: Parkinsons disease, NK cells, CD56bright subset, Disease prognosis, Cytokines

CLC Number: 

  • R741.04
[1] Croisier E, Moran LB, Dexter DT, et al. Microglial inflammation in the parkinsoniansubstantianigra: relationship to alpha-synuclein deposition[J]. J Neuroinflammation, 2005, 2:14.doi: 10.1186/1742-2094-2-14.
[2] Markaki I, Bergstrom S, Tsitsi P, et al. Cerebrospinal fluid levels of kininogen-1 indicateearly cognitive impairment in parkinsons disease[J]. Mov Disord, 2020, 35(11):2101-2106.
[3] Glass CK, Saijo K, Winner B, et al. Mechanisms underlying inflammation in neurodegeneration[J]. Cell, 2010, 140(6):918-934.
[4] Baltus THL, Morelli NR, de Farias CC, et al. Association of -94 ATTG insertion/deletion NFkB1 and c.*126G>A NFkBIA genetic polymorphisms with oxidative and nitrosative stress biomarkers in Brazilian subjects with Parkinsons Disease[J]. Neurosci Lett, 2020, 740:135487.doi:10.1016/j.neulet.2020.135487.
[5] Lahooti B, Chhibber T, Bagchi S, et al. Therapeutic role of inflammasome inhibitors in neurodegenerative disorders[J]. Brain Behav Immun, 2021, 91:771-783. doi: 10.1016/j.bbi.2020.11.004.
[6] Jiang S, Gao H, Luo Q, et al. The correlation of lymphocyte subsets, natural killer cell, and Parkinsons disease: a meta-analysis[J]. Neurol Sci, 2017, 38(8):1373-1380.
[7] Yang M, Zhou Y, Liu L, et al. Decreased A20 expression on circulating CD56(bright)NK cells contributes to a worse disease status in patients with ankylosing spondylitis[J]. Clin Exp Immunol, 2019, 198(1):1-10.
[8] Ilie OD, Ciobica A, McKenna J, et al. Minireview on the relations between gut microfloraand parkinsons disease: further biochemical(oxidative stress), inflammatory, and neurological particularities[J]. Oxid Med Cell Longev, 2020, 2020:4518023.doi: 10.1155/2020/4518023.
[9] Rees K, Stowe R, Patel S, et al. Helicobacter pylori eradication for Parkinsons disease[J]. Cochrane Database Syst Rev, 2011,(11):CD008453.doi:10.1002/14651858.CD008453.pub2.
[10] Tan AH, Mahadeva S, Thalha AM, et al. Small intestinal bacterial overgrowth in Parkinsons disease[J]. Parkinsonism Relat Disord, 2014, 20(5):535-540.
[11] 张忠霞, 马晓伟, 王彦永, 等. 帕金森病模型小鼠黑质纹状体系统氧化应激的增龄性改变[J]. 山东大学学报(医学版), 2014, 52(9): 26-29. ZHANG Zhongxia, MA Xiaowei, WANG Yanyong, et al. Impact of aging on the nigro-strital oxidative stress in a mice model of Parkinsons disease[J].Journal of Shandong University(Health Sciences), 2014, 52(9): 26-29.
[12] Matheoud D, Cannon T, Voisin A, et al. Intestinal infection triggers Parkinsons disease-like symptoms in Pink1(-/-)mice[J]. Nature, 2019, 571(7766):565-569.
[13] 陈思, 王牧川, 任楠楠,等. Parkin基因多态性与山东省汉族帕金森病发病风险的关系[J]. 山东大学学报(医学版), 2015, 53(4): 71-74. CHEN Si, WANG Muchuan, REN Nannan, et al. Relationship between polymorphisms of Parkin gene and the risk of Parkinsons disease in Han nationality of Shandong Province[J]. Journal of Shandong University(Health Sciences), 2015, 53(4): 71-74.
[14] Hirsch EC, Hunot S. Neuroinflammation in Parkinsons disease: a target for neuroprotection?[J]. Lancet Neurol, 2009, 8(4):382-397.
[15] Teismann P, Ferger B. Inhibition of the cyclooxygenase isoenzymes COX-1 and COX-2 provide neuroprotection in the MPTP-mouse model of Parkinsons disease[J]. Synapse, 2001, 39(2):167-174.
[16] Qin XY, Zhang SP, Cao C, et al. Aberrations in peripheral inflammatory cytokine levels in P arkinsondisease: asystematic review and meta-analysis[J]. JAMA Neurol, 2016, 73(11):1316-1324.
[17] Bokor M, Farago A, Garam T, et al. Antibody-dependent cell-mediated cytotoxicity(ADCC)in Parkinsons disease[J]. J Neurol Sci, 1993, 115(1):47-50.
[18] Vandenhaute J, Wouters CH, Matthys P. Natural killer cells in systemic autoinflammatory diseases: afocus on systemic juvenile idiopathic arthritis and macrophage activation syndrome[J]. Front Immunol, 2019, 10:3089.doi:10.3389/fimmu.2019.03089.
[19] Li T, Gao N, Cui W, et al. Natural killer cells and their function in Takayasus arteritis[J]. Clin Exp Rheumatol, 2020, 124(2):84-90.
[20] Cooper MA, Fehniger TA, Turner SC, et al. Human natural killer cells: a unique innate immunoregulatory role for the CD56(bright)subset[J]. Blood, 2001, 97(10):3146-3151.
[21] 张伟, 王政洁, 闫越颖,等. Tim-3对早期梅毒患者外周血CD56dim NK细胞的影响及初步机制[J]. 中国免疫学杂志, 2019, 35(16):2005-2010. ZHANG Wei, WANG Zhengjie, YAN Yueying, et al. Expression of Tim-3 on peripheral CD56dim NK cells of patients with earlysyphilis and its preliminary mechanisms[J]. Chinese Journal of Immunology, 2019, 35(16):2005-2010.
[22] Luci C, Gaudy-Marqueste C, Rouzaire P, et al. Peripheral natural killer cells exhibit qualitative and quantitative changes in patients with psoriasis and atopic dermatitis[J]. Br J Dermatol, 2012, 166(4):789-796.
[23] Jiang W, Chai NR, Maric D, et al. Unexpected role for granzyme K in CD56bright NK cell-mediated immunoregulation of multiple sclerosis[J]. J Immunol, 2011, 187(2):781-790.
[1] LI Tao, YANG Chunlin, DU Tong, LI Heng, WANG Congcong, LI Xiaoli, DUAN Ruisheng,ZHANG Peng. Effects of diabetes mellitus on the subsets and function of NK cells in myasthenia gravis [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 31-36.
[2] ZHANG Xiufang, LI Peizheng, ZHANG Bohan, SUN Congcong, LIU Yiming. Protective effect and mechanism of growth differentiation factor-15 in LPS-induced models of Parkinsons disease [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 1-7.
[3] ZHAO Hailong, WANG Hao, FANG Yuqing, MAO Fei, ZHAO Zhangning, TIAN Xiangqi, XU Xinrong, WANG Min, LI Xiuhua. Efficacy of additional idebenone in the treatment of 34 patients with Parkinsons disease with depression [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 38-44.
[4] LIU Liwen, MA Jun, LI Peizheng, ZHANG Xiufang, LIU Yiming. Caregiver burden and influencing factors of 128 patients with Parkinsons disease [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 45-49.
[5] ZHANG Weiwei, HUA Fang, LIANG Chaoshuai, CHU Miaomiao, SUN Jiayi, FRANK Zaucke, XIN Wei. Thyroid stimulating hormone promotes chondrocyte differentiation via anti-inflammatory protein CTRP3 [J]. Journal of Shandong University (Health Sciences), 2022, 60(10): 1-8.
[6] Shaohua GE,Tian DING,Hongrui LIU. Role and regulatory mechanism of type 2 immunity in tissue repair [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 51-56.
[7] Jing SHAN,Xiaoqiu WANG,Dajin LI. Molecular mechanisms of NK cells involved in endometriosis and its associated infertility [J]. Journal of Shandong University (Health Sciences), 2021, 59(8): 8-13.
[8] LI Ning, WANG Cuilan, ZHANG Yurong. Association analysis of cerebral artery Doppler index and serum EGF level with arteriosclerosis and cognitive impairment in 60 patients with Parkinsons disease [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 61-66.
[9] GUO Pei, LI Xiuhua, ZHANG Xiaotao, HE Tianqi, ZHU Meijia, TANG Jiyou, ZHAO Zhangning, MAO Fei. Clinical characteristics and influencing factors of sleep disorders in patients with Parkinsons disease [J]. Journal of Shandong University (Health Sciences), 2018, 56(4): 76-80.
[10] WANG Qingshi, CHEN Yunzhen, LIU Haichun, WU Wenliang, JIAO Guangjun, LI Xiaofeng, XU Daxia. Effects of inflammatory cytokines secreted by nucleus pulposus cells on proliferation and osteogenic ability of posterior longitudinal ligament fibroblasts [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 22-26.
[11] YUAN Bing, LI Ranran, HAN Mingyong. Primary tumor regulates the pulmonary microenvironment in melanoma carcinoma model and facilitates lung metastasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(11): 13-18.
[12] LI Xiuhua, LI Xiaoli, DUAN Ruisheng, ZHU Meijia, CAO Lili, LI Yanbin, WANG Si, YUE Longtao, MA Qinghai, LIU Fei. Effects of 1,25(OH)2D3 on the induction of immune tolerance in Lewis rats with EAMG [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 5-10.
[13] YANG Yong-rong, LI Jun, LIANG Ye, LI Jin-du, LAN Jia-fu, LI Tian-zi. The study on association of TNF-α-238G/A gene polymorphisms in patients with oral lichen planus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(S1): 10-11.
[14] CHEN Guoling, REN Ran, ZHANG Yinghui, SONG Wei, KAO Xin, ZHANG Han. Significance and effect of emodin on activation of nuclear factor-κB in corneas of keratitis rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 44-47.
[15] WU Fengjuan, LI Tao, QI Qian, LI Yu. Effects of azithromycin and dexamethasone on the secretion of ICAM-1 and TNF-α in A549 cells stimulated with Aspergillus fumigatus conidia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 25-28.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] LYU Longfei, LI Lin, LI Shuhai, QI Lei, LU Ming, CHENG Chuanle, TIAN Hui. Application of laparoscopic fine needle catheter jejunostomy in minimally invasive McKeown resection of esophageal cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 77 -81 .
[2] SHAO Haigang, WANG Xuan, WANG Qing. Anatomy of the root canal system of mandibular first premolar in population of Shandong Province[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 85 -89 .
[3] SHI Shuang, LI Juan, MI Qi, WANG Yunshan, DU Lutao, WANG Chuanxin. Construction and application of a miRNAs prognostic risk assessment model of gastric cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 47 -52 .
[4] TANG Fang1,2, ZHANG Yingqian3, WANG Zhiqiang4, KANG Dianmin4,
WANG Jiezhen1, XUE Fuzhong1. A 2D minimal spanning tree model of the spatial structures of natural focal disease[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(01): 106 -110 .
[5] HUANG Fei,WANG Huaijing,XING Yi,GAO Wei,LI Yonggang,XING Ziying,LI Zhenzhong. Protective effects of NGF and GM1 on primary sensory neurons in SD rat with sciatic nerve injury[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(4): 332 -335 .
[6] LI Yu-liang,WANG Yong-zheng,WANG Xiao-hua,ZHANG Fu-jun,ZHU Li-dong,ZHANG Wang-ming,LI Zheng,LI Zhen-jia,ZHANG Kai-xian. I implantation combined with Gemcitabine in the treatment of advanced pancreatic cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(4): 393 -396 .
[7] LIU Hai-Chun, ZHANG Jian-Feng, CHEN Yuan-zhen. Bone collagen and biomechanical propertyies in the femur of osteoporosis rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(5): 42 -45 .
[8] FENG Fuli1, WEI Shuzhen2, ZHANG Yonghuan3, LI Li1, CHEN Rong1, LIRuifeng1. Relationship between high mortality and altered expression of Klotho in insulin resistance rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(6): 5 -8 .
[9] ZHU Xiaoli1, GUO Shuling1, SU Lei1, FENG Yuxin2, YUAN Fangshu1. Extraction of total proteins from demodex and qualification of their molecular weights[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(5): 58 -62 .
[10] WANG Xiaoju1，　WANG Mingming2，　XU Wansu2,　ZHAO Shengmei3，CUI Sunan2，　LI Xiaoying2，　LIU Chunhua1
. Expression of peripheral blood lymphocyte ubiquitin mRNA in chronic HBV infection with active inflammation and its clinical significance[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 58 -61 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd