Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (10): 18-24.doi: 10.6040/j.issn.1671-7554.0.2020.1585

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Localization and expression of L-type calcium channel in mice primary endolymph sac epithelium

SUI Rongcui1, HAN Shuhui1, ZHANG Xianzhao2, FAN Xintai1, WANG Na1, HOU Lingxiao1, XU Anting1   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, The second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, Shandong, China;
    2. Department of Otorhinolaryngology Head and Neck Surgery, Jining No.1 Peoples Hospital, Jining 272100, Shandong, China
  • Published:2021-10-15

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Abstract: Objective To investigate the localization and expression of voltage-gated calcium channels in primary epithelial cells of the endolymphatic sac in C57BlL/6 mice. Methods Eight of all 60 C57BL/6 mices, 2 days post portum(P2B6), were sacrificed under anesthesia to obtain temporal bone specimens. The endolymphatic sac was located and primary cells were extracted to culture in low serum. The other 52 mice were treated as above. The temporal bone specimens were fixed with 4% formaldehyde and decalcified and embedded. The primary endolymphatic sac epithelial cells were characterized with immunohistofluorescence and immunocytofluorescence, and the expression of L-type calcium channels were determined. Results The endolymphatic sac epithelial cells were tightly arranged in P2B6 mice. The cells could clustered as ridge-like protrusions, which were mainly flat epithelium with small and elliptical cell bodies. The cell culture showed stone-like irregular paving, with different cell shapes and sizes, rich cytoplasm, and oval, large nucleuses. Immunofluorescence showed that the L-type calcium channel protein was uniformly expressed on the endolymphatic sac epithelial cell membrane, and highly expressed in the nucleuses. Conclusion The expression of L-type voltage-dependent calcium channel alpha 1C subunit(CACNA1C), L-type voltage-dependent calcium channel alpha 1S subunit(CACNA1S), and L-type voltage-dependent calcium channel alpha 1D subunit(Cav1.3/CACNA1D)are positive in primary endolymphatic sac epithelial cells of C57BL/6 mice, which provides a basis for cell function experiments and helps to explore the mechanism of endolymphatic sac in maintaining homeostasis.

Key words: Endolymphatic sac primary epithelial cells, L-type calcium channel, Immunofluorescence, Mice

CLC Number: 

  • R392.32
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