Journal of Shandong University (Health Sciences) ›› 2020, Vol. 1 ›› Issue (7): 1-6.doi: 10.6040/j.issn.1671-7554.0.2020.0392

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Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury

SUO Dongyang1, SHEN Fei1, GUO Hao2, LIU Lichang1, YANG Huimin1, YANG Xiangdong1   

  1. 1. Department of Nephropathy, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Blood Purification Center, High-tech Zone Branch Hospital, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250000, Shandong, China
  • Online:2020-07-20 Published:2020-07-10

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Abstract: Objective To explore the role of T cell immunoglobulin domain and mucin domain protein-3(Tim-3)in acute kidney injury(AKI)and study its mechanism. Methods Six to eight weeks old C57 male mice were randomly divided into the control group(CTRL group), cisplatin group(AKI group), and cisplatin + Tim-3 group(RMT group), 4 in each group. Cisplatin(20 mg/kg)was injected intraperitoneally into the mice in the AKI group to construct the AKI animal model, and kidney injury and Tim-3 expression were detected. In RMT group, the antibody RMT3-23(200 μg/per mouse)was used to block the Tim-3 molecule and to establish an AKI model, and then the kidney injury in the AKI and RMT groups and the expressions of Tim-3 and autophagy-related molecules were detected. Results The renal proximal tubules were damaged. Western blotting results showed that the expression level of Tim-3 in AKI group was higher than that in CTRL group (t=3.876, P=0.008 2). Compared with the AKI group, the expression levels of NGAL and P62 in the RMT group increased without the statistical difference(tNGAL=1.664, P=0.157 0; tP62=1.991, P=0.103 1), while the expression of LC3II decreased with a statistical difference(t=5.901, P=0.002 0). The phagocytosis was inhibited and the renal tubular damage was aggravated(P=0.010 1). Conclusion Tim-3 can reduce AKI renal injury induced by cisplatin by regulating the autophagic activity of renal tubular epithelial cells.

Key words: T cell immunoglobulin domain and mucin domain protein-3, Acute kidney injury, Cisplatin, Autophagy, Mouse

CLC Number: 

  • R574
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