Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (1): 62-69.doi: 10.6040/j.issn.1671-7554.0.2017.379

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Effects and mechanisms of hepatoma-derived growth factor on the proliferation of prostate cancer cells

YANG Feilong1,2, ZHOU Zunlin1, REN Juchao1, YAN Lei1, LIU Hainan1, ZHANG Wenhua1, YU Nengwang1, LI Dawei1, XU Zhonghua1   

  1. 1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Key Laboratory of Cardiovascular Remodeling and Function Research, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To study the effect and mechanism of human hepatoma-derived growth factor(hHDGF)on the proliferation of prostate cancer cells. Methods Lentivirus vector was constructed according to the target sequence of hHDGF gene, which was then packaged into lentivirus particles and purified to transfect prostate cancer cells DU145 and LNCaP. The cells were divided into two groups separately: (1) Control group, which was treated by lentivirus with the insignificant sequence; (2) Experimental group, which was treated by the lentivirus with the target sequence. Relative mRNA expression of hHDGF was evaluated by quantitative real-time polymerase chain reaction(qRT-PCR). CCK-8 assay and colony formation assay were performed to measure cell proliferation ability. Relative expressions of hHDGF, phosphatidylinositol 3-kinase(PI3K), phospho-serine/threonine protein kinase(P-AKT)and phosphorylated mammalian 山 东 大 学 学 报 (医 学 版)56卷1期 -杨飞龙,等.肝癌衍生生长因子对前列腺癌细胞增殖的影响及其机制 \=-target of rapamycin(P-mTOR)proteins were detected by Western blotting. Results Lentivirus vector was successfully constructed, packaged and stably transfected into prostate cancer cells. qRT-PCR and Western blotting revealed that the mRNA and protein expression of hHDGF in experimental group were both downregulated(P<0.05). CCK-8 assay and colony formation assay indicated that cell proliferation of experimental group was inhibited(P<0.05). Western blotting revealed that relative protein expression of PI3K, P-AKT and P-mTOR were all downregulated(P<0.05). Conclusion Cell proliferation of proatate cancer is inhibited by the downregulation of hHDGF expression, which may be related with the inhibition of PI3K-AKT-mTOR signaling way.

Key words: Hepatoma-derived growth factor, Prostate neoplasm, Cell proliferation, PI3K-AKT-mTOR signaling way, DU145, LNCaP

CLC Number: 

  • R737.25
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