JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (5): 12-16.doi: 10.6040/j.issn.1671-7554.0.2015.1257

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Expression of histone methyltransferase SMYD3 in prostate cancer tissues and its role in mTOR pathway

XIA Chuanyou, LI Luchao, LI Xiaofeng, YAN Keqiang, ZHANG Nianzhao, FAN Yidong, LIU Cheng   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-12-08 Online:2016-05-16 Published:2016-05-16

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Abstract: Objective To determine the expression of histone methyltransferase SMYD3 in prostate cancer tissues and to explore its role and mechanism in prostate cancer progression. Methods The expression of SMYD3 in 33 pairs of prostate cancer tissues and matched non-tumor tissues were detected with immunohistochemical staining. Prostate cancer cell line LNCaP and PC3 were transfected with siRNA and SMYD3 over-expressing vectors, respectively, to evaluate their growth. The expressions of proteins involved in mTOR pathway were evaluated with Western blotting. Results Prostate cancer had over-expressed SMYD3 compared with non-tumor normal tissues. The SMYD3 expression could be found in both nucleus and cytoplasm. SMYD3 abundance had a positive correlation with cell proliferation. The number of LNCaP cells was lower in SMYD3 siRNA group compared with the control group, and the number of PC3 cells was higher in SMYD3-PC vector group than in the control group. The depletion of the enzyme could down-regulate the expression of p-mTOR(P<0.05)and p-p70S6K(P<0.05), while the expression of p-4EBP-1(P<0.05)could be up-regulated; the trend of protein expression in mTOR pathway in PC3 was similar to that in LNCaP. Conclusion SMYD3 is over-expressed in prostate cancer tissues compared with non-tumor tissues, and SMYD3 can be expressed both in nucleus and cytoplasm. The expression of SMYD3 can activate the growth of prostate cancer cells by activating mTOR pathway.

Key words: Prostate neoplasms, MTOR pathway, SiRNA, SMYD3

CLC Number: 

  • R737.25
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