JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (1): 1-4.doi: 10.6040/j.issn.1671-7554.0.2013.383

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Effect of CD40siRNA on CD4+CD25+ regulatory T cells in rats with experimental autoimmune myocarditis

WANG Jie-zhong1, HAN Bo1, GAO Ling2, YANG Wen-wei1, LIU Feng-qin1, Lv-Jian-li1, Zhao Li-jian1   

  1. 1. Department of Pediatric Cardiology; 2. Central Laboratory, Shandong Provincial Hospital Affiliated to
    Shandong University, Jinan 250021, China
  • Received:2013-06-14 Online:2014-01-10 Published:2014-01-10

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Abstract:

 Objective  To investigate the therapeutic effect of CD40 siRNA on viral myocarditis and its effect on CD4+CD25+ regulatory T cells (Treg). Methods  A total of 40 male Lewis rats were randomly divided into four groups: normal control group, experimental autoimmune myocarditis (EAM) model group, CD40 siRNA treatment group and siRNA treatment group, with 10 rats in each group. On the 1st and 8th day, 0.2mL PBS buffer was injected subcutaneously into the two hind footpads of rats in the control group, while 0.2mL porcine cardiac myosin was injected subcutaneously into the two hind footpads of rats in the other three groups. On the 8th day, 25μL CD40siRNA lentiviral expression vector was injected into the tail vein of rats in the CD40siRNA treatment group, and 25μL siRNA lentiviral expression vector was injected into the tail vein of rats in the siRNA group. After all rats were sacrificed on the 21st day, myocardial pathological changes were observed with optical microscope, and CD4+CD25+Treg expression in rats’ spleen was determined with flow cytometry. Results  The myocardial histopathology integral of the CD40siRNA treatment group (11.70 ± 2.95) was significantly lower than that of the EAM model group (17.00 ± 1.76) (P<0.05). The CD4+CD25+Treg expression was significantly higher in the CD40siRNA treatment group (40.70±4.00) than in the EAM model group (12.20±1.10) (P<0.05). Conclusion  CD40siRNA can relieve myocarditis in EAM rats. The mechanism may be related to the up-regulation of CD4+CD25+Treg expression.

Key words: Myocarditis, EAM rats, CD40siRNA, Regulatory T cells

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