Abstract: Objective  To investigate the effect of SIRT3 gene on the expressions of inflammatory factors intercellular cell adhesion molecule-1 (ICAM-1) and E-selectin in human aortic endothelial cell (HAEC) stimulated by oxidized-low density lipoprotein(ox-LDL). Methods  The cells were randomly divided into normal control group, transfection reagent control group, negative control group, and SIRT3 siRNA (1, 2, 3 and 4) groups. When the most effective interference sequence was selected out, the cells were divided into normal control group, siRNA group, (20, 30 and 40μg/mL) ox-LDL groups and siRNA+(20, 30 and 40μg/mL) ox-LDL groups. The cells were transfected for 24h and stimulated by ox-LDL for 6h. SIRT3 mRNA level was detected by qRT-PCR and its protein level was analyzed by Western blotting.  Mean fluorescence intensities (MFIs) of ICAM-1 and E-selectin antibody were detected by flow  cytometry. Results  SIRT3siRNA2 was the most effective interference sequence. The interference efficiency of SIRT3siRNA2 was 88.62% in mRNA level and 80.37% in protein level. Expressions of SIRT3 mRNA had significant differences in ox-LDL groups (all P＜0.05) compared with that in normal control group; with the increase of ox-LDL concentration, the expression of SIRT3 mRNA gradually declined (all P＜0.05). MFI of ICAM-1 and E-selectin had statistical differences in siRNA+ox-LDL groups compared with that in siRNA group (all P＜0.05); with the increase of ox-LDL concentration, the MFI was enhanced obviously (all P＜0.05). MFI of ICAM-1 and E-selectin in siRNA group had no significant difference compared with that in normal control group (P＞0.05). Conclusion  The SIRT3 gene has association with the expressive increments of  E-selectin and ICAM-1 in HAEC stimulated by ox-LDL, and may regulate the inflammatory reaction in artery endothelial cell injury process.

Key words: E-selectin, siRNA, SIRT3, Human aortic endothelial cell, Oxidized-low density lipoproteinSIRT3, Intercellular cell adhesion molecule-1