Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 13-17.doi: 10.6040/j.issn.1671-7554.0.2017.163

Previous Articles     Next Articles

Effects of Vitamin D on the content of Ceramide in rat adrenal pheochromocytoma PC12 Cells

GUAN Xinyuan, GAO Jifang, GUO Juanjuan, LIU Shilian   

  1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shandong University, Jinan 250012, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

PDF (PC)

18

Abstract: Objective To invastigate the effect of vitamin D(VD)on the content of ceramide(Cer)in rat adrenal pheochromocytoma PC12 cells. Methods MTT was performed to verify the effect of short-chain C2-Cer(20 μmol/L)on cell proliferation. High performance liquid chromatography tandem mass spectrometry(HPLC-MS)was performed to detect the concentration of C16: 0-Cer in PC12 cells stimulated with VD(50 or 0 nmol/L). qRT-PCR was performed to measure the mRNA expression levels of enzymes which were related to the metabolism of Cer in PC12 cells after being stimulated with VD(50 or 0 nmol/L). Western blotting was performed to measure the protein levels of SPTLC1 and Cers6 in PC12 cells that were stimulated with VD(200, 100, 50, 25, 12.5 and 0 nmol/L). Results Activity of PC12 cells was inhibited by C2-Cer with IC50 being 2.800 μmol/L. Compared with VD at 0 nmol/L, the content of C16: 0-Cer in PC12 cells was decreased by VD at 50 nmol/L ［(19.37±0.61)ng/mL vs(16.50±0.10)ng/mL, P=0.010］, the expressions of SPTLC1 mRNA(P=0.001)and Cers6 mRNA(P<0.001)in PC12 cells were increased, the protein expression of SPTLC1 in PC12 cells was reduced(P=0.032)and the protein expression of Cers6 was increased(P=0.020). Conclusion The concentration of C16:0-Cer is decreased in PC12 cells that were treated with VD, and it may be related to the decrease of protein expression of SPTLC1.

Key words: Ceramide, PC12 cell, Vitamin D, High pressure liquid chrometography /mass spectroscopy

CLC Number: 

  • R744.5+1
[1] Grigoriadis N, Van Pesch V. A basic overview of multiple sclerosis immunopathology[J]. Eur J Neurol, 2015, 22(2):3-13.
[2] Dyment DA, Yee IM, Ebers GC. Multiple sclerosis in stepsiblings: recurrence risk and ascertainmen[J]. J Neurol Neurosurg Psychiatry, 2006, 77(2):258-259.
[3] Beecham AH, Patsopoulos NA, Xifara DK. International multiple slerosis genetics consortium(IMSGC), analysis of immune-related loci identifies 48 new susceptibility variants for multiple sclerosis[J]. Nat Genet, 2013, 45(11):1353-1360.
[4] Cross AH, Naismith RT. Established and novel disease-modifying treatments in multiple sclerosis[J]. J Intern Med, 2014, 275(4):350-363.
[5] Smolders J, Damoiseaux J, Menheere P. Vitamin D as an immune modulator in multiple sclerosis, a review[J]. J Neuroimmunology, 2008, 194(1): 7-17.
[6] Scott TF, Hackett CT, Dworek DC. Low vitamin D level is associated with higher relapse rate in natalizumab treated MS patients[J]. J Neurol Sci, 2013, 330(1-2): 27-31.
[7] Martinelli V, Dalla Costa G, Colombo B. Vitamin D levels and risk of multiple sclerosis in patients with clinically isolated syndromes[J]. Mult Scler, 2014, 20(2):147-155.
[8] Spach KM, Hayes CE. Vitamin D3 confers protcttion from autimmune encephalomyelit is only in female mice[J]. J Immunol, 2005, 175(6):4119-4126.
[9] Gomez-Mu(~overn)oz A, Presa N, Gomez-Larrauri A. Control of inflammatory responses by ceramide, sphingosine 1-phosphate and ceramide 1-phosphate[J]. Prog Lipid Res, 2016, 61:51-62. doi: 10.1016/j.plipres.2015.09.002.
[10] Dai L, Trillo-Tinoco J, Bai A, et al. Ceramides promote apoptosis for virus-infected lymphoma cells through induction of ceramide synthases and viral lytic gene expression[J]. Oncotarget, 2015, 6(27): 24246-24260.
[11] Saddoughi SA, Ogretmen B. Diverse functions of ceramide in cancer cell death and proliferation[J]. Adv Cancer Res, 2013, 117:37-58. doi: 10.1016/B978-0-12-394274- 6.00002-9.
[12] Susanne S, Ferreiros N, Birod K. Ceramide synthase 6 plays a critical role in the development of experimental autoimmune encephalomyelitis[J]. J Immunol, 2012, 188(11):5723-5733.
[13] Zhu Y, Qin Z, Gao J. Vitamin D therapy in experimental allergic encephalomyelitis could be limited by opposing effects of sphingosine-1-phosphate and gelsolin dysregulation[J]. Mol Neurobiol, 2014, 50(3):733-743.
[14] Hoveizi E, Tavakol S, Ebrahimi-Barough S. Neuroprotective effect of transplanted neural precursors embedded on PLA/CS scaffold in an animal model of multiple sclerosis[J]. Mol Neurobiol, 2015, 51(3):1334-1342.
[15] Ohno Yusuke, Nakamichi S, Ohkuni A. Essential role of the cytochrome P450 CYP4F22 in the production of acylceramide, the key lipid for skin permeability barrier formation[J]. PNAS, 2015, 112(25):7707-7712.
[16] Macchi B, Marino-Merlo F, Nocentini U, et al. Role of inflammation and apoptosis in multiple sclerosis: comparative analysis between the periphery and the central nervous system[J]. J Neuroimmunol, 2015, 287:80-87. doi:10.1016/j.jneuroim.2015.08.016.
[17] Hartfield PJ, Manyne GC, Murray AW. Ceramide induces apoptosis in PC12 cells[J]. FEBS Letters, 1997, 401(2-3):148-152.
[18] Ben-David O, Futerman AH. The role of the ceramide acyl chain length in neurodegeneration: involvement of ceramide synthases[J]. Neuromolecular Med, 2010, 12(4):341-350.
[19] Hojjati MR, Li Z, Jiang XC. Serine palmitoyl-CoA transferase(SPT)deficiency and sphingolipid levels in mice[J]. Biochim Biophys Acta, 2005, 1737(1):44-51.
[20] Michael M, Sarah S. Sphingolipid metabolites in inflammatory disease[J]. Nature, 2014, 510(7503): 58-67.
[1] Ping LIU,Yuwen SONG,Ping WANG,Guangwei TIAN,Fengjie ZHENG,Li LYU,Jiaojiao DU,Jing ZHANG,Xianghua ZHUANG,Shihong CHEN. Correlation between vitamin D deficiency and depression in patients with type 2 diabetes mellitus [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 51-56,102.
[2] SHI Rui, SUN Pei, WANG Lulu, DING Lin, XIA Jin, WANG Yan, PANG Shuguang. Vitamin D and mecobalamin combined with mouse nerve growth factor for diabetic peripheral neuropathy: a clinical observation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 64-67.
[3] WANG Zhen, GAO Jifang, GUAN Xinyuan, GUO Juanjuan, YANG Duxiao, LIU Shilian. Effects of Vd on SPHK1/S1P pathway in PC12 cells and its potential role in EAE [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 21-24.
[4] HOU Lijun1, XING Lijian2, LI Guanghong1, LIU Yan3, QI Wenbo4. Association between vitamin D receptor gene ApaI polymorphism and impaired glucose tolerance [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 51-54.
[5] ZHANG Yanling1, ZHANG Xu1, ZHANG Wenwen1, WANG Furong2, YU Chunxiao1, GUAN Qingbo1, YU Guina1, GAO Ling3, ZHAO Jiajun1, XU Jin1. Relationship between serum level of 25-hydroxyvitamin D and fasting blood glucose in healthy adults of Jinan [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(3): 59-63.
[6] FENG Xiao-wen, GAO Qing, DONG Hai-man, PENG Lei, YUE Qing-wei, ZHANG Jing, BAO Li-hua, LI Gui-bao, SUN Jin-hao, GAO Ying-mao. Protective effect of resveratrol on Aβ25-35-induced PC12 cell injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(4): 56-60.
[7] MIAO Yu-e, WANG Jue, WANG Wei-bo. Expression of vitamin D receptor in gastric carcinoma and
normal mucosa adjacent to carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(2): 74-.
[8] YIN Xiao1, YAN Ling2, LU Yong1. Effects of vitamin D intervention on obesity and  its related metabolic disorders [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 1-.
[9] YIN Xiao1, YAN Ling2, LU Yong1. Effects of vitamin D intervention on obesity and  its related metabolic disorders [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 1-.
[10] WANG Xiang-Yu1，MEI Yuan-Wu2，YANG Hong-Wei3. Protective effect of curcumin on dopamineinduced apoptosis in PC12 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 29-33.
[11] WANG Yan, KANG Dong-hong, CAO Wei, WANG Ping, LIU Zhi-wen. Relationship between serum 25-hydroxyvitamin D and the quality of life in middle-aged and older people [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 1-.
[12] LI Xiaohong1,2, LI Yun1, WANG Banqin1, LIU Shilian1. Association of DBP gene polymorphism and risk of multiple sclerosis in Han people of Shandong Province [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 67-.
[13] LIU Li, CUI Dong-Sheng, CUI Hui-Xian, GU Ping, GENG Yuan, WANG Ming-Wei. Expression of prostate apoptosis response4 in PC12 cells 
after retonone treatment [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(5): 34-37.
[14] ZENG Ji-ping,WANG Li-na,WANG Li-xiang,REN Xiao-hui,ZHANG Meng-ye,XIA Wen,CUI Xing. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(5): 467-470.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24 -31 .
[2] GUO Zhihua, ZHAO Daqing, XING Yuan, WANG Wei, LIANG Leping, YANG Jing, ZHAO Qianqian. Single-stage end-to-end anastomosis in the management of severe cervical tracheal stenosis[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 72 -76 .
[3] Di ZHANG,Meng YU,Xia LIU. A general overview of neuromodulation[J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 50 -60 .
[4] DU Yong,LI Yu,JIANG Peng. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(5): 519 -523 .
[5] . [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(4): 372 -375 .
[6] Xingang LI,Xin ZHANG,Anjing CHEN. The latest advances in human brain projects[J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 5 -9, 21 .
[7] ZHU Xuguo1,2,3, YU Guanying3, LI Yaonan1,2,3, LIU Lei1, ZHANG Jixun1, LIU Zhenzhong1, JIANG Duyin1,2,3. Initial exploration on the recycling of deep second-degree burn skin for constructing model of complex skin transplanting[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(4): 53 -57 .
[8] MIAO Jun-dong,LIU Wei-hua,REN Man-yi,SUI Shu-jian. The QRS interval for cardiac insufficiency following acute myocardial infarction[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(2): 188 -191 .
[9] HAN Ting, DU Jing, YU Jiali, ZHENG Wenzhou, WANG Yan. Factors influencing root resorption after replantation of avulsed permanent teeth: a report of 47 teeth[J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 115 -119 .
[10] PAN Hong,WANG Li-hua,MA Lu-xin. Pentacam and IOLMaster in keratometry and anterior chamber depth measurements: a contrast study[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(6): 624 -627 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd