Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 13-17.doi: 10.6040/j.issn.1671-7554.0.2017.163

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Effects of Vitamin D on the content of Ceramide in rat adrenal pheochromocytoma PC12 Cells

GUAN Xinyuan, GAO Jifang, GUO Juanjuan, LIU Shilian   

  1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shandong University, Jinan 250012, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

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Abstract: Objective To invastigate the effect of vitamin D(VD)on the content of ceramide(Cer)in rat adrenal pheochromocytoma PC12 cells. Methods MTT was performed to verify the effect of short-chain C2-Cer(20 μmol/L)on cell proliferation. High performance liquid chromatography tandem mass spectrometry(HPLC-MS)was performed to detect the concentration of C16: 0-Cer in PC12 cells stimulated with VD(50 or 0 nmol/L). qRT-PCR was performed to measure the mRNA expression levels of enzymes which were related to the metabolism of Cer in PC12 cells after being stimulated with VD(50 or 0 nmol/L). Western blotting was performed to measure the protein levels of SPTLC1 and Cers6 in PC12 cells that were stimulated with VD(200, 100, 50, 25, 12.5 and 0 nmol/L). Results Activity of PC12 cells was inhibited by C2-Cer with IC50 being 2.800 μmol/L. Compared with VD at 0 nmol/L, the content of C16: 0-Cer in PC12 cells was decreased by VD at 50 nmol/L ［(19.37±0.61)ng/mL vs(16.50±0.10)ng/mL, P=0.010］, the expressions of SPTLC1 mRNA(P=0.001)and Cers6 mRNA(P<0.001)in PC12 cells were increased, the protein expression of SPTLC1 in PC12 cells was reduced(P=0.032)and the protein expression of Cers6 was increased(P=0.020). Conclusion The concentration of C16:0-Cer is decreased in PC12 cells that were treated with VD, and it may be related to the decrease of protein expression of SPTLC1.

Key words: Ceramide, PC12 cell, Vitamin D, High pressure liquid chrometography /mass spectroscopy

CLC Number: 

  • R744.5+1
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