JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 21-24.doi: 10.6040/j.issn.1671-7554.0.2016.224

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Effects of Vd on SPHK1/S1P pathway in PC12 cells and its potential role in EAE

WANG Zhen, GAO Jifang, GUAN Xinyuan, GUO Juanjuan, YANG Duxiao, LIU Shilian   

  1. Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-03-04 Online:2016-10-10 Published:2016-10-10

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Abstract: Objective To investigate the effect of vitamin D(Vd)on experimental autoimmune encephalomyelitis(EAE)through the regulation of sphingosine kinase type 1(SPHK1)on the content of sphingosine-1-phosphate(S1P). Methods PC12 cells cultured in vitro and stimulated with different concentrations of Vd were divided into 6 groups: 0 nmol/L Vd group, 6.25 nmol/L Vd group, 12.5 nmol/L Vd group, 25 nmol/L Vd group, 50 nmol/L Vd group, 100nmol/L Vd group. The SPHK1 levels were analyzed with Western blotting. The expression of SPHK1 mRNA was detected with RT-PCR. The intracellular S1P was detected with ELISA. Results After PC12 cells were stimulated with different concentrations of Vd for 24h, an obvious change was observed between Vd groups and 0 nmol/L Vd group. Compared with 0nmol/L Vd group, the Vd groups had decreased levels of SPHK1, S1P and SPHK1 mRNA(P<0.05). Conclusion Vd might affect the content of S1P via SPHK1 so as to delay the progression of EAE.

Key words: PC12 cells, Vitamin D, Sphingosine kinase type 1, Sphingosine 1-phosphate, Experimental autoimmune encephalomyelitis

CLC Number: 

  • R744.5+1
[1] Dorr J, Doring A, Paul F. Can we prevent or treat multiple sclerosis by individualised vitamin D supply?[J]. EPMAJ, 2013, 4(1):4.doi:10.1186/1878-5085-4-4.
[2] Smolders J, Damoiseaux J, Menheere P, et al. Vitamin D as an immune modulator in multiple sclerosis, a review [J]. J Neuroimmunol, 2008, 194(1-2):7-17.
[3] Pierrot-deseilligny C. Clinical implications of a possible role of vitamin D in multiple sclerosis [J]. J Neurol, 2009, 256(9):1468-1479.
[4] Klampfer L, Huang J, Sasazuki T, et al. Oncogenic Ras promotes butyrate-induced apoptosis through inhibition of gelsolin expression [J]. J Biol Chem, 2004, 279(35):36680-36688.
[5] Zhu Y, Qin Z, Gao J, et al. Vitamin D therapy in experimental allergic encephalomyelitis could be limited by opposing effects of sphingosine 1-phosphate and gelsolin dysregulation[J]. Mol Neurobiol, 2014, 50(3):733-743.
[6] Pebay A, Toutant M, Premont J, et al. Sphingosine-1-phosphate induces proliferation of astrocytes: regulation by intracellular signalling cascades [J]. Eur J Neurosci, 2001, 13(12):2067-2076.
[7] Sorensen SD, Nicole O, Peavy RD, et al. Common signaling pathways link activation of murine PAR-1, LPA, and S1P receptors to proliferation of astrocytes [J]. Mol Pharmacol, 2003, 64(5):1199-1209.
[8] Spach KM, Hayes CE. Vitamin D3 confers protection from autoimmune encephalomyelitis only in female mice [J]. J Immunol, 2005, 175(6):4119-4126.
[9] Hoveizi E, Tavakol S, Ebrahimi-barough S. Neuroprotective effect of transplanted neural precursors embedded on PLA/CS scaffold in an animal model of multiple sclerosis [J]. Mol Neurobiol, 2015, 51(3):1334-1342.
[10] Ambrosini E, Remoli ME, Giacomini E, et al. Astrocytes produce dendritic cell-attracting chemokines in vitro and in multiple sclerosis lesions[J]. J Neuropathol Exp Neurol, 2005, 64(8):706-715.
[11] Meyer R, Weissert R, Diem R, et al. Acute neuronal apoptosis in a rat model of multiple sclerosis[J]. J Neurosci, 2001, 21(16):6214-6220.
[12] Peterson JW, Bo L, Mork S, et al. Transected neurites, apoptotic neurons, and reduced inflammation in cortical multiple sclerosis lesions [J]. Ann Neurol, 2001, 50(3):389-400.
[13] Kulakowska A, Zendzian-piotrowska M, Baranowski M, et al. Intrathecal increase of sphingosine 1-phosphate at early stage multiple sclerosis[J]. Neurosci Lett, 2010, 477(3):149-152.
[14] Cantorna MT, Hayes CE, Deluca HF. 1,25-Dihydroxyvitamin D3 reversibly blocks the progression of relapsing encephalomyelitis, a model of multiple sclerosis[J]. Proc Nati Acad Sci USA, 1996, 93(15):7861-7864.
[15] Embry AF, Smowdon LR, Vieth R. Vitamin D and seasonal fluctuations of gadolinium-enhancing magnetic resonance imaging lesions in multiple sclerosis [J]. Ann Neurol, 2000, 48(2):271-272.
[16] 兰天, 常秀亭, 勾红菊,等. SphK1-S1P信号通路的激活参与糖尿病大鼠肾损伤的研究[J]. 重庆医学, 2013, 42(22):2571-2573. LAN Tian, CHANG Xiuting, GOU Hongju, et al. Activation of Sphk1-S1P signaling pathway is involved in diabetic rat renal injury [J]. Chongqing Medicine, 2013, 42(22):2571-2573.
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