JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 25-28.doi: 10.6040/j.issn.1671-7554.0.2016.587

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Changes of hydrogen sulfide concentrations and synthetase expressions in the corpus cavernosum of diabetic rats

ZHAO Huichen1,2*, CHAI Jiachao3,4*, ZHANG Liang5, YUAN Mingzhen6, PENG Li7, LIU Yuantao1   

  1. 1. Department of Endocrinology, Qingdao Municipal Hospital, Qingdao 266071, Shandong, China;
    2. Department of Nephrology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    3. Department of Pediatric Orthopaedics, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    4. Department of General Surgery, Qingdao Women and Childrens Hospital, Qingdao 266011, Shandong, China;
    5. Department of Endocrinology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    6. Department of Urology, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    7. Cadres Ward, Jinan Fourth Peoples Hospital, Jinan 250031, Shandong, China
  • Received:2016-05-23 Online:2016-10-10 Published:2016-10-10

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Abstract: Objective To examine the endogenous hydrogen sulfide(H2S)concentrations and expressions of cystathionine-γ-lyase(CSE)in the corpus cavernosum tissues of diabetic and non-diabetic rats. Methods Rats were randomly divided into two groups. One group was fed with high-fat diet for 8 weeks and injected with streptozocin(STZ)to create type 2 diabetic rat models. The other group was fed with normal diet to serve as controls. All mice were killed and corpus cavernosum tissues were harvested. After that H2S concentrations in corpus cavernosum tissues were measured. The mRNA and protein expressions of CSE were detected with RT-PCR and Western blotting respectively. Results 山 东 大 学 学 报 (医 学 版)54卷10期 -赵蕙琛,等.糖尿病大鼠阴茎海绵体硫化氢含量及其合成酶表达的变化 \=-The H2S level in the corpus cavernosum tissues was significantly decreased in the diabetic group compared with the controls(P<0.01). The mRNA and protein expressions of CSE were significantly decreased in the diabetic group(both P<0.05). Conclusion H2S concentration was significantly decreased in the corpus cavernosum tissues of diabetic rats, which may be associated with the down-regulation of mRNA and protein expressions of CSE.

Key words: Diabetes, Cystathionine-γ-lyase, Erectile dysfunction, Hydrogen sulfide, Rat

CLC Number: 

  • R587.2
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