JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 16-20.doi: 10.6040/j.issn.1671-7554.0.2015.1029

Previous Articles     Next Articles

Effect of umbilical cord mesenchymal stem cells on microglial polarization and proliferation

WANG Na, CHEN Naiyao   

  1. Department of Hematology, Affiliated Hospital of North China University of Science and Technology, Tangshan 063000, Hebei, China
  • Received:2015-10-29 Online:2016-10-10 Published:2016-10-10

PDF (PC)

255

Abstract: Objective To observe the effect of umbilical cord mesenchymal stem cells(hUC-MSCs)on the microglia proliferation and activation in normal or inflammatory conditions, in order to explore their immunomodulation effect on the central nervous system. Methods After BV2 cells were co-cultured with hUC-MSCs-CM and/or lipopolysaccharide(LPS)for 24 or 48 h, the proliferation was analyzed by MTT colorimetric method, TNF-α and IL-6 in the supernatant were measured by ELISA kit, Arginase 1(Arg1)protein and mRNA levels were examined by Western blotting, TNF-α and IL-6 mRNA expression level were tested by real-time PCR. Results (1) After long-time LPS stimulation, BV2 cells obviously proliferated, while hUC-MSCs-CM inhibited the proliferation. (2) BV2 cultured with LPS expressed high levels of TNF-α and IL-6, while in both hUC-MSCs-CM and LPS conditions, the TNF-α and IL-6 levels were lower than in LPS stimulation. (3) The level of Arg1 was increased in BV2 cells co-cultured with hUC-MSCs-CM for 48 h. Conclusions Human UC-MSCs inhibit microglia proliferation caused by inflammatory stimulation. Human UC-MSCs induce microglial cells to M2 polarization and reduce the expression of proinflammatory factors via paracrine mechanism, and this may be the mechanism for its neuroprotective effect.

Key words: Neuroprotection, Immunomodulation, Microglia, Umbilical cord mesenchymal stem cells

CLC Number: 

  • R392.1
[1] Saijo K, Glass CK. Microglial cell origin and phenotypes in health and disease[J]. Nat Rev Immunol, 2011, 11(11): 775-787.
[2] Lee WH, Sonntag WE, Mitschelen M, et al. Irradiation induces regionally specific alterations in pro-inflammatory environments in rat brain[J]. Int J Radiat Biol, 2010, 86(2): 132-144.
[3] Jenrow KA, Brown SL, Lapanowski K, et al. Selective inhibition of microglia-mediated neuroinflammation mitigates radiation-induced cognitive impairment[J]. Radiat Res, 2013, 179(5): 549-556.
[4] Mosser DM, Edwards JP. Exploring the full spectrum of macrophage activation[J]. Nat Rev Immunol, 2008, 8(12): 958-969.
[5] Chhor V, Le Charpentier T, Lebon S, et al. Characterization of phenotype markers and neuronotoxic potential of polarised primary microglia in vitro[J]. Brain Behav Immun, 2013, 32:70-85. doi: 10.1016/j.bbi.2013.02.005.
[6] Tang Y, Le W. Differential roles of M1 and M2 microglia in neurodegenerative diseases[J]. Mol Neurobiol, 2016, 53(2): 1181-1194.
[7] Hegyi B, Kornyei Z, Ferenczi S, et al. Regulation of mouse microglia activation and effector functions by bone marrow-derived mesenchymal stem cells[J]. Stem Cells Dev, 2014, 23(21): 2600-2612.
[8] Beltrami AP, Cesselli D, Bergamin N, et al. Multipotent cells can be generated in vitro from several adult human organs(heart, liver, and bone marrow)[J]. Blood, 2007, 110(9): 3438-3446.
[9] Gao S, Mao F, Zhang B, et al. Mouse bone marrow-derived mesenchymal stem cells induce macrophage M2 polarization through the nuclear factor-kappaB and signal transducer and activator of transcription 3 pathways[J]. Exp Biol Med(Maywood), 2014, 239(3): 366-375.
[10] Ennis WJ, Sui A, Bartholomew A. Stem cells and healing: impact on inflammation[J]. Adv Wound Care(New Rochelle), 2013, 2(7): 369-378.
[11] Kharraz Y, Guerra J, Mann CJ, et al. Macrophage plasticity and the role of inflammation in skeletal muscle repair[J]. Mediators Inflamm, 2013: 491497. doi: 10.1155/2013/491497.
[12] Mandal P, Pratt BT, Barnes M, et al. Molecular mechanism for adiponectin-dependent M2 macrophage polarization: Link between the metabolic and innate immune activity of full-length adiponectin[J]. J Biol Chem, 2011, 286(15): 13460-13469.
[13] Busch SA, Silver J. The role of extracellular matrix in CNS regeneration[J]. Curr Opin Neurobiol, 2007, 17(1): 120-127.
[14] Tang Y, Li T, Li J, et al. Jmjd3 is essential for the epigenetic modulation of microglia phenotypes in the immune pathogenesis of Parkinsons disease[J]. Cell Death Differ, 2014, 21(3): 369-380.
[15] Rath M, Muller I, Kropf P, et al. Metabolism via Arginase or Nitric Oxide Synthase: Two Competing Arginine Pathways in Macrophages[J]. Front Immunol, 2014, 5: 532. doi: 10.3389/fimmu.2014.00532
[1] YU Yujuan, GAO Chengjie, WANG Xiaopeng, LI Bo. Effects of parecoxibsodium on inflammatory response and its neuroprotection in patients undergoing brain tumor surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 65-68.
[2] LIU Ning, L(¨overU)Xin, LI Dong, HUNAG Zhiwei, LIU Yi, ZHANG Leling. Construction and identification of lentiviral vector for angiopoietin-1 gene and its expression in human umbilical cord mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(12): 1-7.
[3] QIAO Shan, HAN Tao, LI Wenna, WANG Shengjun, ZHAO Xiuhe, YANG Xue, LIU Xuewu. Neuroprotective effects of histone deacetylases inhibitor 11r on rats following status epilepticus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(6): 39-43.
[4] LI Guiting, ZHANG Rui, ZOU Shanshan, DING Ming. Effects of ketamine on the learning and memory ability as well as the TSPO protein expression in hippocampus in juvenile mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 55-60.
[5] YUAN Ruili, SUN Ruopeng, LIU Xinjie. Caspase-1 activation contributes to Nod2-induced tolerance in microglia cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 34-38.
[6] LI Mei, MENG Qinghui, CAI Qiaoying, XU Yan, FAN Xiaoting. Effect of acetylpuerarin on the expression of caspase-3 in BV-2 microglia induced by Aβ25-35 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(10): 32-36.
[7] LIU Juan, HAN Guoqing, LIU Hui, QIN Chengyong. Mechanism of human umbilical cord mesenchymal stem cells suppressing cholangiocarcinoma cell growth [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 1-5.
[8] HAN Xiaojuan1, MA Xue-qiang2, DU Yi-feng1. Effects of Piper Kadsura Ohwi extracts on β-amyloid protein-induced activation of microglia in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 6-10.
[9] QI Shen, HAN Xiao-juan, WANG Lu, ZHANG Qing-hua, DU Yi-feng. Effects of Piper Kadsura Ohwi extracts on IL-1β and TNF-α  expressions in activated microglia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(5): 11-14.
[10] SHANG Xiao1, CUI Yuan-xiao1, LIU Xiao-yu1, FENG Xiao-ya2. Experimental study of human umbilical cord mesenchymal stem cells combined with minimal hematoma aspiration in the therapy of rats with intracerebral hemorrhage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2013, 51(10): 10-14.
[11] ZHANG Hui, GUO Yu-ji, HAO Ai-jun, MA Bao-hua. Effect of Granulocyte-colony stimulating factor on microglial function in
the acute spinal cord injury mice model [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(5): 10-.
[12] WEN Xian-yu， MAO qiao， CUI Chun-ai. Effects of nutmeg extraction on microglial BV2 cells treated with IFN-γ [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 21-.
[13] WANG Mei-xia, LIU Xue-ping, XU Song, DONG Chuan-fang, HOU Liang, YUAN Shu-hua. Effect of advanced glycation end products on interleukin-1β and tumor  necrosis factor α secretion from microglial cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 34-38.
[14] YANG Li-ling， WANG Lu， REN Xiao-yan， HAN Xiao-juan， DU Yi-feng. JAK/STAT signaling pathway mediates β-amyloid protein-induced TNF-α action of microglia in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(7): 33-36.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd