JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 49-54.doi: 10.6040/j.issn.1671-7554.0.2014.818

Previous Articles     Next Articles

Effects of theanine on the proliferation and differentiation of neural stem cells

LIANG Yu1, XIN Tao1, LIU Qian2, ZHENG Xiangrong1, YANG Yihang1, PANG Qi1   

  1. 1. Department of Neurosurgery, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Histology and Embryology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-12 Revised:2015-03-09 Online:2015-04-10 Published:2015-04-10

PDF (PC)

177

Abstract: Objective To explore the effects of theanine on the proliferation and differentiation of neural stem cells (NSCs). Methods NSCs were isolated from Kunming mouse embryonic at E12.5 by mechanical blowing combined with stencils of 200 mesh. Isolated NSCs were then identified with nestin immunofluorescent staining technique. Different concentrations of theanine were added into nutrient fluid, cell activity at different time was detected with MTS, and the final concentration of theanine (100 μmol/L) was determined. Then NSCs were divided into two groups: control group, theanine (100 μmol/L) treated group. The proliferation and differentiation of NSCs were determined with BrdU labeling and immunofluorescent staining of microtubule associated protein 2 (MAP2) and glial fibrillary acidic protein (GFAP). The Q-PCR and Western blotting were used to detect the expression of MAP2 and GFAP. Results The optical density of theanine (100 μmol/L) treated group was significantly increased in MTS (P <0.05). The ratio of BrdU-positive cells and MAP2-positive cells against total NSCs treated with theanine was significantly increased in comparison with that in control group (P <0.05), but there was no noticeable difference in GFAP immunofluorescence between the two groups. The expression of MAP2 treated with theanine was significantly increased compared with that in the control group, there was no noticeable difference in GFAP expression between the two groups. Conclusion Theanine (100 μmol/L) can promote the proliferation of NSCs and its neuronal differentiation.

Key words: Proliferation, Differentiation, Mice, Theanine, Neural stem cells

CLC Number: 

  • R34
[1] Meade AJ, Meloni BP, Mastaglia FL, et al. AP-1 inhibitory peptides attenuate in vitro cortical neuronal cell death induced by kainic acid[J]. Brain Res, 2010, 1360: 8-16.
[2] Revishchin AV, Korochkin LI, Okhotin VE, et al. Neural stem cells in the mammalian brain[J]. Int Rev Cytol, 2008, 265: 55-109.
[3] Falk S, Sommer L. Stage- and area-specific control of stem cells in the developing nervous system[J]. Curr Opin Genet Dev, 2009, 19(5): 454-460.
[4] 孟红旗, 全亚萍. 神经干细胞在神经系统疾病中的应用[J]. 中国组织工程研究与临床康复, 2010, 14(1): 175-178. MENG Hongqi, QUAN Yaping. Application of neural stem cells in nervous system diseases[J]. Journal of Clinical Rehabilitaitve Tissue Engineering Research, 2010, 14(1): 175-178.
[5] Desai MJ, Gill MS, Hsu WH, et al. Pharmacokinetics of theanine enantiomers in rats[J]. Chirality, 2005, 17(3): 154-162.
[6] Cho HS, Kim S, Lee SY, et al. Protective effect of the green tea component, L-theanine on environmental toxins-induced neuronal cell death[J]. Neurotoxicology, 2008, 29(4): 656-662.
[7] 熊方令, 高宜录. 内源性神经干细胞修复中枢神经系统损伤的策略[J]. 基础医学与临床, 2010, 30(10): 1105-1108. XIONG Fangling, GAO Yilu. Strategy to repair central lesion by endogenous neural stem cells[J]. Basic & Clinical Medicine, 2010, 30(10): 1105-1108.
[8] Suh H, Deng W, Gage FH, et al. Signaling in adult neurogenesis[J]. Annu Rev Cell Dev Biol, 2009, 25: 253-275.
[9] Unno T, Suzuki Y, Kakuda T, et al. Metabolism of theanine,gamma-glutamylethylamide, in rats[J]. J Agric Food Chem, 1999, 47(4): 1593-1596.
[10] Borzelleca JF, Peters D, Hall W, et al. A 13-week dietary toxicity and toxicokinetic study with l-theanine in rats[J]. Food Chem Toxicol, 2006, 44(7): 1158-1166.
[11] 陈淑珍, 甄永苏. 茶氨酸的药理学作用及机制[J]. 医学研究杂志, 2013, 42(11): 17-20.
[12] Nagasawa K, Aoki H, Yasuda E, et al. Possible involvement of group I mGluRs in neuroprotective effect of theanine[J]. Biochem Biophys Res Commun, 2004, 320(1): 116-122.
[13] Takahashi M, Okubo N, Chosa N, et al. Fibroblast growth factor-1-induced ERK1/2 signaling reciprocally regulates proliferation and smooth muscle cell differentiation of ligament-derived endothelial progenitor cell-like cells[J]. Int J Mol Med, 2012, 29(3): 357-364.
[14] Lonic A, Powell JA, Kong Y, et al. Phosphorylation of serine 779 in fibroblast growth factor receptor 1 and 2 by protein kinase C (epsilon) regulates Ras/mitogen-activated protein kinase signaling and neuronal differentiation[J]. J Biol Chem, 2013, 288(21): 14874-14885.
[15] Phoenix TN, Temple S. Spred1, a negative regulator of Ras-MAPK-ERK, is enriched in CNS germinal zones, dampens NSC proliferation, and maintains ventricular zone structure[J]. Genes Dev, 2010(1): 45-56.
[16] Sainz RM, Mayo JC, Tan DX, et al. Antioxidant activity of melatonin in Chinese hamster ovarian cells: changes in cellular proliferation and differentiation[J]. Biochem Biophys Res Commun, 2003, 302(3): 625-634.
[17] Cowles MW, Brown DD, Nisperos SV, et al. Genome-wide analysis of the bHLH gene family in planarians identifies factors required for adult neurogenesis and neuronal regeneration[J]. Development, 2013, 140(23): 4691-4702.
[18] Fu J, Zhao SD, Liu HJ, et al. Melatonin promotes proliferation and differentiation of neural stem cells subjected to hypoxia in vitro[J]. J Pineal Res, 2011, 51(1): 104-112.
[1] DUAN Chu-Hong, LIU Kai, YIN Hai-Yan, ZHAO Shi-Dou, LIU Feng-Tao. Effects of lectin receptors of WGA,RCA and ECL on the development of cleft palate induced by excess retinoid acid in KM mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 47-.
[2] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[3] SUN Yifeng, GAO Yu, LIANG Yongyuan, GAO Yang. Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 34-39.
[4] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7-14.
[5] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32-37.
[6] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38-46.
[7] WANG Kaimin, TAN Juanjuan, YAN Zhiqiang, LI Zhiqiang. Effects of Sirtuin on hypoxia-induced proliferation of human umbilical cord-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 23-30.
[8] LI Jingyuan, SONG Ling, LIN Song, SONG Hui. Effects of carbon monoxide releasing molecule-3 on the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 31-37.
[9] LI Shanshan, YANG Jing, ZHANG Jin. Post-transcriptional regulation mechanism of sclerostin in mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 43-48.
[10] JIAO Fengping, WANG Yu, YU Ailian, TIAN Zhaoju, YANG Shulin. Immunogenicity of the eukaryotic expression vector pcDNA3.1(-)-P6-gBCTL-TBK-1 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 75-78.
[11] SHI Peikun, ZENG Beini, WU Weifang, HU Xiaoyan, MA Tianjia, ZHOU Yabin. Expression and role of Keap1 in renal cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 94-99.
[12] SUN Zeyu, CHEN Ying, LIN Jiaxiang, LIU Juan, ZHAO Weiming. Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 1-6.
[13] WANG Tiantian, CHEN Jie. Expression and clinical significance of EFEMP2 in endometrial cancer tissues and cell [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 52-58.
[14] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[15] TIAN Siqi, LIU Riqiang, YANG Ning, WEI Wei, YANG Huawei. Effect of expression of ribosomal protein S6 Kinase 4 variants on the proliferation of breast cancer cell MDA-MB-231 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 32-36.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd