JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (7): 22-27.doi: 10.6040/j.issn.1671-7554.0.2014.175

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Inhibitory effect of insulin on prostate cancer LNCaP cell line

WANG Chen, TANG Yueqing, YAN Lei, JIAO Wei, ZHOU Zunlin, XU Zhonghua   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-03-28 Revised:2014-05-27 Online:2014-07-10 Published:2014-07-10

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Abstract: Objective To study the inhibitory effect of insulin on LNCaP human prostate cancer cell line and its possible mechanism. Methods After LNCaP cells were treated with insulin, the expression of insulin receptor was detected with Real-time PCR, immunocytochemistry and Western blotting. The effects of insulin on cell growth, viability, migration and invasion were measured by cell counting, MTT, wound healing and Transwell. The activation of MAPK pathway was determined by Western blotting. Results Insulin increased the mRNA and protein expressions of INSR in LNCaP cells, and inhibited the cell growth, viability, migration and invasion. Expressions of key proteins in the MAPK pathway, including Ras, p-MEK and p-ERK, were significantly lowered by insulin. Conclusion Insulin can inhibit prostate cancer LNCaP cell line through MAPK signal pathway.

Key words: Insulin receptor, Prostatic neoplasms, MAPK pathway, Insulin

CLC Number: 

  • R737.25
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