JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Neuroprotective effects of insulinlike growth factor 1 on cultured dorsal
root ganglion neurons with neurotoxicity induced by glutamate

ZHANG Fengjun, WANG Lihong, LIU Zhen, XING Ziying, WANG Huaijing, LI Zhenzhong
   

  1. (Department of Anatomy, School of Medicine, Shandong University, Jinan 250012, China)
  • Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
  • Contact: LI Zhenzhong

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Abstract: To determine the neuroprotective effects of insulinlike growth factor 1 (IGF1) on cultured dorsal root ganglion (DRG) neurons injured by glutamate (Glu). MethodsDRG neurons of Wistar rat embryos were cultured for 48?h in vitro and then exposed to Glu (200?μmol/L) with or without IGF1 (10?nmol/L). The living cells were observed with an inverted contrast microscope. The cultures were processed for detecting the apoptosis rate using flow cytometry and for detecting the fluorescent intensity of intracellular Ca2+ using confocal laser scanning microscopy (CLSM). ResultsSurvival status of the DRG cells with IGF1 incubation was better than that without IGF1 incubated cultures. The apoptosis rate and fluorescent intensity of Ca2+ were lower in cultures with IGF1 than those without. ConclusionIGF1 may reduce intracellular Ca2+ concentration, inhibits neuronal apoptosis, and protects the DRG neurons from neurotoxicity induced by Glu.

Key words: Insulinlike growth factor 1, Ganglion, Neuron, Glutamate, Rat,Wistar

CLC Number: 

  • R322.85
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