Abstract:

Objective  To observe the destructive effects of 1-three chlorine methyl-l,2,3,4-four hydrogenation of beta carboline (Taclo) on the dopaminergic neurons in the substantia nigra after striatal injection and search for a new method to establish parkinson disease (PD) rat model. Methods  Sixty Wistar male rats were randomly divided into polyethylene glycol (PEG) and Taclo groups, 30 in each group. Apomorphine-induced rotation was observed two weeks after injection and rats′ trajectory was recorded by the automatic video tracking system. The rats were sacrificed one month later and tyrosine hydroxylase (TH) immunohistochemistry was conducted. Results  Twenty-four rats constantly rotated to the left side at a speed above 210r/30min in Taclo group. The trajectory became sparse in these rats. The Results   of immunohistochemistry showed that the number of TH-positive neurons in right substantia nigra of rats in Taclo group decreased than those in left substantia nigra and bilateral substantia nigra of rats in PEG group (P<0.05). Twenty-four rats can be regarded as successful PD rat models. Conclusion  PD rat model can be established by injecting Taclo sterically and directionally into the striatum.

Key words: Parkinson disease, 1-three chlorine methyl-l,2,3,4-four hydrogenation of beta carboline, Rat model, Dopaminergic neuron in substantia nigra