JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (3): 19-22.doi: 10.6040/j.issn.1671-7554.0.2013.607

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Metabolic features of glioma cells observed by proton magnetic resonance spectroscopy at high strength magnetic field

ZHANG Zhaotao1, ZHANG Hua2, HE Jingzhen1, ZHU Jianhui3, LIU Yun1,  WANG Jianzhen1, LI Chuanfu1, ZENG Qingshi1   

  1. 1. Department of Radiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong， China;
    2. Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan 250062, Shandong，  China;
    3. Department of Radiology, Maigaiti Hospital of Xinjiang, Maigaiti 844600, Xinjiang， China
  • Received:2013-10-11 Online:2014-03-10 Published:2014-03-10

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Abstract:

To observe the metabolic features of glioma cells by proton magnetic resonance spectroscopy (1H-MRS) at 14.7T high strength magnetic field. Methods  Glioma cell lines C6 and  U251 were cultivated. Water-soluble metabolites were extracted by adding perchloric acid. 1H-MRS at 14.7T magnetic field was acquired and special metabolic ratios were calculated, including lactate (Lac) / creatine (Cr), succinate (Suc) / Cr, N-acetylaspartate (NAA) / Cr, choline (Cho) / Cr, Cho / NAA, Glycine (Gly) / Cr, and myo-inositol (MI) / Cr. Results  C6 and U251 cell lines had almost the same water-soluble metabolites but different metabolic ratios. Conclusion  MRS can be used to analyze cells’ metabolic features and monitor physiological or pathological state at the cell level.

Key words: Proton magnetic resonance spectroscopy, Brain glioma cells, Water-soluble metabolites

CLC Number: 

  • R574
[1] XU Yanjie, CUI Yi, LI Hongxia, SHI Wenqi, LI Fuyan, WANG Jianzhen, ZENG Qingshi. Effect of X-ray on the activity of MMP-2 and Cho/Cr ratio of U87 glioma multicellular spheroid [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 6-10.
[2] HU Fangzhi, ZHANG Zhengjun, GENG Houfa, LIANG Qiuhua, SUN Lin. Relationship between microalbuminuria and brain damage in patients with type 2 diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(2): 43-47.
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