JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (3): 5-8.doi: 10.6040/j.issn.1671-7554.0.2015.409

Previous Articles     Next Articles

Effects of oxygen with different concentrations on the expressions of S100β protein and neuron-specific enolase after cardiopulmonary resuscitation in rats

WANG Limin1, WANG Xudong2   

  1. 1. Postgraduate Training Base;
    2. Department of Emergency, Aerospace Central Hospital of Liaoning Medical University, Beijing 100049, China
  • Received:2015-04-23 Online:2016-03-10 Published:2016-03-10

PDF (PC)

335

Abstract: Objective To investigate the effects of oxygen with different concentrations on the outcome of cardiopulmonary resuscitation(CPR)in rats. Methods Thirty-two SD rats were randomly divided into the control group and resuscitation group, which was subdivided into 21% oxygen concentration group, 50% oxygen concentration group and 100% oxygen concentration group, with 8 in each group. Anesthesia, tracheal intubation, and vascular puncturation were performed for the control group. Other groups were given 21%, 50% and 100% oxygen respectively. ELISA assay was used to detect the levels of of serum S100β protein and neuron-specific enolase(NES)at 0.5, 3, and 6 h after successful CPR. Results Compared with the control group, the level of serum S100β protein in resuscitation group was higher(P<0.05)and reached the peak at 3h time point, while the level of serum NSE rose stably with the increase of time(P<0.05). After a successful resuscitation, the risen levels of S100β protein and NSE were as follows: 21% oxygen concentration group > 100% oxygen concentration group > 50% oxygen concentration group(P<0.05). Conclusion Compared with 21% and 100% oxygen, 50% oxygen inhalation can decrease the levels of serum S100β protein and NSE after a successful CPR, minimize secondary brain injury and thus improve the brain functionality.

Key words: Neuron-specific enolase, Cardiopulmonary resuscitation, S100β protein, Cardiac arrest

CLC Number: 

  • R459.7
[1] Xu J, Sun S, Lu X, et al. Remote ischemic pre- and postconditioning improve postresuscitation myocardial and cerebral function in a rat model of cardiac arrest and resuscitation[J]. Crit Care Med, 2015, 43(1): e12-e18.
[2] Miyata K, Mikami T, Asai Y, et al. Subarachnoid hemorrhage after resuscitation from out-of-hospital cardiac arrest[J]. J Stroke Cerebrovasc Dis, 2014, 23(3): 446-452.
[3] 谷兴华,张供,张希全,等.血清蛋白B亚型和神经元特异性烯醇化酶对早期判断体外循环术后脑损伤的意义[J].中华医学杂志, 2007, 87(14): 975-977. GU XingHua, ZHANG Gong, ZHANG Xiquan, et al. Clinical values of detection of serum levels of S100B and NSE in diagnosis of brain injuries at early period after cardiopulmonary bypass[J]. Natl Med J China, 2007, 87(14): 975-977.
[4] Sramko M, Peichl P, Wichterle D, et al. A novel biomarker-based approach for the detection of asymptomatic brain injury during catheter ablation of atrial fibrillation[J]. J Cardiovasc Electrophysiol, 2014, 25(4): 349-354.
[5] Martínez-Morillo E, García Hernández P, Begcevic I, et al. Identification of novel biomarkers of brain damage in patients with hemorrhagic stroke by integrating bioinformatics and mass spectrometry-based proteomics[J]. J Proteome Res, 2014, 13(2): 969-981.
[6] Rohlwink UK, Figaji AA. Biomarkers of brain injury in cerebral infections[J]. Clin Chem, 2014, 60(6): 823-834.
[7] Thel MC, OConnor CM. Cardiopulmonary resuscitation: historical perspective to recent investigations[J]. Am Heart J, 1999, 137(1): 39-48.
[8] Peberdy MA, Kaye W, Ornato JP, et al. Cardiopulmonary resuscitation of adults in the hospital: a report of 14720 cardiac arrests from the National Registry of Cardiopulmonary Resuscitation[J]. Resuscitation, 2003, 58(3): 297-308.
[9] 沈洪,王士雯. 2003:中国心肺复苏的关注问题[J].中国危重病急救医学, 2003, 15(5): 262-264.
[10] 李媛媛,张楠,徐谧山,等.新生小鼠缺氧缺血脑组织基质细胞衍生因子1α的表达及其作用[J].山东大学学报(医学版), 2015, 53(10): 26-31. LI Yuanyuan, ZHANG Nan, XU Mishan, et al. Expressing of stromal cell derived factor-1α and its effects in hypoxic-ischemic brain tissue of neonatal mice[J]. Journal of Shandong University(Health Science), 2015, 53(10): 26-31.
[11] Wheeler KT, Payne V, DAgostino RB Jr, et al. Impact of breathing 100% oxygen on radiation-induced cognitive impairment[J]. Radiat Res, 2014, 182(5): 580-585.
[12] 孙斌,缪珀,任静,等.空气及纯氧对新生儿复苏效果的Meta分析[J].中国血液流变学杂志, 2014, 24(1): 110-114. SUN Bin, MIAO Po, REN Jing, et al. Effect of air and 100% oxygen resuscitation of newborn: a Meta-analysis[J]. Chinese Journal of Hemorheology, 2014, 24(1): 110-114.
[13] 陈素艳,高峰,张清勇,等.不同浓度常压氧对大鼠脑缺血再灌注损伤早期的保护作用[J].中华实验外科杂志, 2014, 31(1): 111-113. CHEN Suyan, GAO Feng, ZHANG Qingyong, et al. Protective effect of different concentrations of normobaric oxygen in early cerebral ischemic-reperfusion injury rat model[J]. Chin J Exp Surg, 2014, 31(1): 111-113.
[14] Clark SD, Saker F, Schneeberger MT, et al. Administration of 100% oxygen does not hasten resolution of symptomatic spontaneous pneumothorax in neonates[J]. J Perinatol, 2014, 34(7): 528-531.
[15] 朱佳骏,姜舟,吴明远.空气或纯氧复苏宫内窒息新生大鼠血气和大脑SOD水平的比较[J].中华急诊医学杂志, 2011, 20(1): 48-51. ZHU Jiajun, JIANG Zhou, WU Mingyuan. Effects of resuscitation with air or oxygen on blood gas and cerebral SOD concentration in neonatal rats with intrauterine asphyxia[J]. Chin J Emerg Med, 2011, 20(1): 48-51.
[16] Solberg R, Enot D, Deigner HP, et al. Metabolomic analyses of plasma reveals new insights into asphyxia and resuscitation in pigs[J]. PLoS One, 2010, 5(3): e9606.
[17] 蒋红斌,黎惟广.不同氧浓度复苏囊正压呼吸在早产儿原发性呼吸暂停中的应用[J].中国基层医药, 2011, 18(18): 2540-2541.
[18] 王珂,张振香,陈素艳,等.45%浓度氧对大鼠缺血再灌注损伤的实验研究[J].中国实用神经疾病杂志, 2013, 16(13): 50-51.
[19] 李神清,熊敏,孙新飞.血清BNP和S100蛋白在心肺复苏后脑损伤判断中的作用[J].国际检验医学杂志, 2014, 35(16): 2254-2256.
[20] Žurek J, Fedora M. The usefulness of S100B, NSE, GFAP, NF-H, secretagogin and Hsp70 as a predictive biomarker of outcome in children with traumatic brain injury[J]. Acta Neurochir(Wien), 2012, 154(1): 93-103.
[21] Larsson IM, Wallin E, Kristofferzon ML, et al. Post-cardiac arrest serum levels of glial fibrillary acidic protein for predicting neurological outcome[J]. Resuscitation, 2014, 85(12): 1654-1661.
[1] LIANG Lining, ZHONG Xia, JI Xianfei, HU Haoran, ZHU Fangfang, CHEN Jibin,CHEN Qianqian, SHANG Deya. Protective effects of post-conditioning with nicorandil on myocardial, cerebral and renal ischemia-reperfusion injury following cardiac arrest in swine [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 46-51.
[2] YU Yujuan, GAO Chengjie, WANG Xiaopeng, LI Bo. Effects of parecoxibsodium on inflammatory response and its neuroprotection in patients undergoing brain tumor surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 65-68.
[3] ZANG Lijiao, QIU Jie, ZHUANG Genmiao, AN Li. Correlation among serum S100B protein, NSE and neonatal hypoglycemic brain damage [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 51-54.
[4] HAN Xiao, LIU Kun, SHI Jiahai, GAO Fumin, YOU Qingsheng. Cerebral protection of Xingnaojing injection in patients undergoing cardiopulmonary bypass surgery [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 54-57.
[5] WANG Bo-song, ZHONG Jing-quan, LI Jing-sha, ZENG Qi-xian, LIU Hong-zhen, SU Guo-ying, LIU Dong-lin， ZHANG Yun. Comparison of effects of continuous and one-shock defibrillations on a  canine model of prolonged ventricular fibrillation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 37-.
[6] WANG Bo-song, ZHONG Jing-quan, LI Jing-sha, ZENG Qi-xian, LIU Hong-zhen, SU Guo-ying, LIU Dong-lin， ZHANG Yun. Comparison of effects of continuous and one-shock defibrillations on a  canine model of prolonged ventricular fibrillation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 37-.
[7] . Coronary perfusion pressure during ventricular fibrillation and waveform-based  indicators for the return of spontaneous circulation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(2): 54-.
[8] MENG Xianglin, ZHONG Jingquan, SU Guoying, ZENG Qixian, LIU Hongzhen，LIU Donglin, WANG Yuanlong, LI Jingsha, ZHANG Yun . Comparison of the effects of two doses of epinephrine during cardiopulmonary  resuscitation  in a swine model of prolonged ventricular fibrillation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 32-35.
[9] ZOU Yan-guo,XU Rong-ting,HE Xu-dong,XING Jie-ling,SUI Wen-juan,SHAO Hua,WANG Xing-lei. Effects of Shengmai Injection on NF-κB in patients after resuscitation of cardiac arrest [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(11): 1127-1130.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd