JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Differential expression of M2PK in normal cervix and cervical cancer tissues

BAI Xiao-hui,KONG Li，ZHANG Ai-rong，JIA Ji-hui1，ZHANG Wei-fang，TANG Wei，YU Xiu-ping   

  1. 1. Institute of Microbiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China； 2. Department of Obstetric and Gynecology, Second Hospital of Shandong University,
  • Received:2005-10-29 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24
  • Contact: BAI Xiao-hui

Abstract: To screen out cervical carcinoma related protein markers by a comparative proteome analysis. Methods: Twodimensional gel electrophoresis maps of the proteins extracted from H8 and Caski were applied respectively. The differential expression proteins were identified by MALDITOFMS; Western blot was used to detect M2PK expression from normal cervix and cervical carcinoma tissues. Results: The 2DE maps of H8 cells and Caski cells were established, and the protein M2PK was identified successfully. Western blot confirmed differential expression of M2PK on normal cervix and cervical carcinoma tissues. Conclusion: These proteins from H8 cells and Caski cells are differentially expressed. It will be helpful to find new molecular markers for early diagnosis and treatment of cervical cancer.

Key words: Proteome, Electrophoresis, gel, twodimensional, Cervix neoplasms, M2type pyruvate kinase, Western blot

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