JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 34-39.doi: 10.6040/j.issn.1671-7554.0.2015.1092

Previous Articles     Next Articles

Preparation and evaluation of 125I-rFlic and 125I-rFlicΔ180-400 in noninvasive radioimaging of allorejection

XUE Ying, ZHANG Chao, LIANG Ting, SONG Jing, HOU Guihua   

  1. Institute of Experimental Nuclear Medicine, Medical School of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-09 Online:2016-10-10 Published:2016-10-10

PDF (PC)

144

Abstract: Objective To evaluate the effect of iodine-125 labeled intact flagellin(125I-rFlic)and its fragment(125I-rFlicΔ180-400)in allograft acute rejection model on non-invasive monitoring allorejection. Methods The rFlic and rFlicΔ180-400 were labeled with iodine-125 with iodogen method, and label rate, stability and receptor binding activities were analyzed. After the allograft/isograft mice models were established, biodistribution, pharmacokinetics and phosphor-autoradiography imaging were detected on day 8 post transplantation after two tracers’ injection through tail vein separately. Results The prepared 125I-rFlic and 125I-rFlicΔ180-400 were stable. Radioligand-based binding assay showed 125I-rFlicΔ180-40 had higher affinity than 125I-rFlic. In ex vivo biodistribution study, 125I-rFlicΔ180-400 showed higher skin graft/opposite-side skin(T/NT)than 125I-rFlic 24 h after tracer injection(P=0.014 8). The pharmacokinetic results indicated the metabolism of125I-rFlicΔ180-400 was faster than that of 125I-rFlic. The whole-body autoradiography showed higher radioactivities in allograft 6 h than 1h after tracers’ injection, and allograft-radioimage of 125I-rFlicΔ180-400 6 h was much clearer than that of 125I-rFlic. Moreover, 125I-rFlic and 125I-rFlicΔ180-400 both showed preferentially graft-accumulated in the allorejection group after 24 h. Conclusion Both 125I-rFlic and 125I-rFlicΔ180-400 can specifically accumulate on graft in allorejection models; the latter is faster metabolized and earlier focused on allograft than the former.

Key words: Radioiodine, Toll-like receptor 5, Flagellin, Allotransplant acute rejection

CLC Number: 

  • R817-33
[1] Bouvy AP, Klepper M, Kho MM, et al. The impact of induction therapy on the homeostasis and function of regulatory T cells in kidney transplantpatients[J]. Nephrol Dial Transplant, 2014, 29(8):1587-1597.
[2] Schumann J, Stanko K, Woertge S, et al. The mitochondrial protein TCAIM regulates activation of T cells and thereby promotes tolerance induction of allogeneic transplants[J]. Am J Transplant, 2014, 14(12):2723-2735.
[3] Bates JT, Graff AH, Phipps JP, et al. Enhanced antigen processing of flagellin fusion proteins promotes the antigen-specific CD8+T cell response independently of TLR5 and MyD88[J]. J Immunol, 2011, 186(11):6255-6262.
[4] Savar NS, Jahanian-Najafabadi A, Mahdavi M, et al. In silico and in vivo studies of truncated forms of flagellin(Flic)of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary tract infections[J]. J Biotechnol, 2014, 175: 31-37. doi: 10.1016/j.jbiotec.2014.01.037.
[5] Liu F, Yang J, Zhang Y, et al. Recombinant flagellins with partial deletions of the hypervariable domain lose antigenicity but not mucosal adjuvancy[J]. Biochem Biophys Res Commun, 2010, 392(4):582-587.
[6] Hao J, Zhang C, Liang T, et al. rFlic prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner[J]. Cell Mol Immunol, 2014, 11(2):206-214.
[7] Zhang C, Hou G, Liang T, et al. A prospective study of macrophage migration inhibitory factor as a marker of inflammatory detection[J]. J Cell Mol Med, 2009, 13(9B): 4077-4083.
[8] Atif SM, Lee SJ, Li LX, et al. Rapid CD4+T-cell responses to bacterial flagellin require dendritic cell expression of Syk and CARD9[J]. Eur J Immunol, 2015, 45(2):513-524.
[9] Sun H, Yang G, Liang T, et al. Non-invasive imaging of allogeneic transplanted skin graft by 131I-anti-TLR5 mAb[J]. J Cell Mol Med, 2014, 18(12):2437-2444.
[10] Yang C, Yun Q, Sun H, et al. Non\-invasive imaging of Toll\-like receptor 5 expression using 131I\-labeled mAb in the mice bearing H22 tumors[J]. Oncol Lett, 2014, 7(6):1919-1924.
[11] Ding H, Zhang H, Ding H, et al. Transplantation of placenta-derived mesenchymal stem cells reduces hypoxic-ischemic brain damage in rats by ameliorating the inflammatory response[J]. Cell Mol Immunol, 2015,28. doi: 10.1038/cmi.2015.99.
[12] Chen DL, Wang X, Yamamoto S, et al. Increased T cell glucose uptake reflects acute rejection in lung grafts[J]. Am J Transplant, 2013, 13(10):2540-2549.
[13] Cheng D, Sun H, Liang T, et al. Noninvasive allograft imaging of acute rejection: evaluation of(131)I-anti-CXCL10 mAb[J]. Inflammation, 2015, 38(1):456-464.
[14] Payne GA, Hage FG, Acharya D. Transplant allograft vasculopathy: Role of multimodality imaging in surveillance and diagnosis[J]. J Nucl Cardiol, 2015, 28.[Epub ahead of print]
[15] Walker CM, Hobbs SB, Carter BW, et al. Dyspnea, Cough, and Abnormal Thoracic Imaging after Lung Transplantation[J]. Ann Am Thorac Soc, 2016, 13(1):134-136. doi:10.1513/AnnalsATS.201509-648CC.
[1] YANG Huan， LIANG Ting， ZHANG Chao， SONG Jing， HAO Jing， HOU Gui-hua. Preparation of 131I-rFliC and its biodistribution
 in breast cancer-bearing mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(12): 31-36.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd