JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 34-39.doi: 10.6040/j.issn.1671-7554.0.2015.1092

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Preparation and evaluation of 125I-rFlic and 125I-rFlicΔ180-400 in noninvasive radioimaging of allorejection

XUE Ying, ZHANG Chao, LIANG Ting, SONG Jing, HOU Guihua   

  1. Institute of Experimental Nuclear Medicine, Medical School of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-09 Online:2016-10-10 Published:2016-10-10

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Abstract: Objective To evaluate the effect of iodine-125 labeled intact flagellin(125I-rFlic)and its fragment(125I-rFlicΔ180-400)in allograft acute rejection model on non-invasive monitoring allorejection. Methods The rFlic and rFlicΔ180-400 were labeled with iodine-125 with iodogen method, and label rate, stability and receptor binding activities were analyzed. After the allograft/isograft mice models were established, biodistribution, pharmacokinetics and phosphor-autoradiography imaging were detected on day 8 post transplantation after two tracers’ injection through tail vein separately. Results The prepared 125I-rFlic and 125I-rFlicΔ180-400 were stable. Radioligand-based binding assay showed 125I-rFlicΔ180-40 had higher affinity than 125I-rFlic. In ex vivo biodistribution study, 125I-rFlicΔ180-400 showed higher skin graft/opposite-side skin(T/NT)than 125I-rFlic 24 h after tracer injection(P=0.014 8). The pharmacokinetic results indicated the metabolism of125I-rFlicΔ180-400 was faster than that of 125I-rFlic. The whole-body autoradiography showed higher radioactivities in allograft 6 h than 1h after tracers’ injection, and allograft-radioimage of 125I-rFlicΔ180-400 6 h was much clearer than that of 125I-rFlic. Moreover, 125I-rFlic and 125I-rFlicΔ180-400 both showed preferentially graft-accumulated in the allorejection group after 24 h. Conclusion Both 125I-rFlic and 125I-rFlicΔ180-400 can specifically accumulate on graft in allorejection models; the latter is faster metabolized and earlier focused on allograft than the former.

Key words: Radioiodine, Toll-like receptor 5, Flagellin, Allotransplant acute rejection

CLC Number: 

  • R817-33
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