JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (2): 74-78.doi: 10.6040/j.issn.1671-7554.0.2015.373

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Antifungal effects and relevant mechanisms of metabolites of Lactobacillus casei against Candida albicans biofilms in vitro

FENG Fan, HU Xiaoyan, WU Weifang, SUN Xiaolin, SUN Yundong, CAO Qian, XIAO Ying, YAN Shikun, SHI Peikun, ZENG Beini, ZHOU Yabin   

  1. Department of Medical Microbiology, Medical School of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-04-13 Online:2017-02-10 Published:2017-02-10

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Abstract: Objective To detect the antifungal effects and relevant mechanisms of metabolites of Lactobacillus casei against Candida albicans biofilms in vitro. Methods Metabolites of Lactobacillus casei were obtained by extraction and distillation. MIC80 of metabolites of Lactobacillus casei against planktonic cells and biofilms were determined with the tetrazolium salt(XTT)reduction assay. The metabolic activity of Candida albicans biofilms against metabolites of Lactobacillus casei was assessed quantitatively. Yeast-to-hypha morphological transition was examined qualitatively with inverted microscope. Two-dimensional gel electrophoresis(2-DE)was used to search differentially expressed protein spots against metabolites of Lactobacillus casei-treated Candida albicans. Results MIC80 of metabolites of Lactobacillus casei against Candida albicans planktonic cells and biofilms were 1∶256 and 1∶128, respectively. When the dilution ratio was 1∶256, metabolites of Lactobacillus casei blocked the yeast-to-hypha transition. Compared with six significant protein spots achieved from the 2-DE maps, four of them were indentified. The expressions of enolase, ubiquitin ligase and malate dehydrogenase were increased, while the expression of hexokinase was decreased under the stimulation of metabolites of Lactobacillus casei. Conclusion Metabolites of Lactobacillus casei can inhibit Candida albicans biofilms in vitro.

Key words: Candida albicans, Metabolite of Lactobacillus casei, Antifungal effects, Biofilm, Two-dimensional gel electrophoresis

CLC Number: 

  • R379.4
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