JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Effects of BRMS1 gene transfection on invasion potential of breast

TANG Lu-bing,SUN Jing-zhong,GAO Hai-dong,WANG Tian-tian   

  1. 1.Department of Breast Surgery; 2.Laboratory of Cardiovascular Disease, Qilu Hospital of Shandong University, Jinan 250012, Shandong， China
  • Received:2006-03-29 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

Abstract: To evaluate the invasion potential of breast cancer cell MDAMB231 transfected by BRMS1 gene in vitro. Methods: A eukaryotic expression vector containing BRMS1 gene was transfected into breast cancer cell MDAMB231 by lipofectamine，and then RTPCR was used to detect the expression of BRMS1mRNA in cells before and after transfection, scanning electron microscope to observe the ultrastructure, and Boyden Chamber to assay the change of invasion. Results: After transfection, BRMS1mRNA was upregulated significantly, the ultrastructure obviously changed, and the ability of invasion significantly dropped off in vitro. Conclusion: BRMS1 gene suppresses the invasion of breast cancer cell MDAMB231 in vitro.

Key words: Breast neoplasms, Genes, BRMS1, Invasiveness

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