JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 77-80.doi: 10.6040/j.issn.1671-7554.0.2014.679

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Antibiotic resistance on group A streptococcus among children in 4 cities of Shandong Province in 2013

LIU Zhenyan1, HU Bin2, BI Zhenwang2, KOU Zengqiang2, FANG Ming2, CHEN Baoli2, REN Yanyan2, BI Zhenqiang1,3   

  1. 1. Department of Epidemiology and Biostatistics, School of Public Health, Shandong University, Jinan 250012, Shandong, China;
    2. Institute for Bacterial Infectious Disease Prevention and Control, Shandong Center for Disease Control and Prevention, Jinan 250014, Shandong, China;
    3. Shandong Center for Disease Control and Prevention, Shandong Key Laboratory of Infectious Disease Prevention and Control, Shandong University Institute of Preventive Medicine, Jinan 250014, Shandong, China
  • Received:2014-10-09 Revised:2014-11-25 Published:2015-01-10

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Abstract: Objective To investigate the characteristics of antibiotic resistance, resistance phenotypes and genotypes on group A streptococcus (GAS) isolated from children in 4 cities of Shandong Province in 2013 and to provide reference for clinical treatment of GAS infection. Methods A total of 72 strains of GAS were isolated from throat swabs of children with scarlet fever or asymptomatic carriers. The resistance of GAS strains on 8 antibiotics, including penicillin, erythromycin, clindamycin, levofloxacin, tetracycline, chloramphenicol, vancomycin and cefotaxime, were measured with K-B disk-diffusion method. DNA was extracted from GAS strains, resistance genes (ermA, ermB, mefA, tetM, tetO) were amplified with PCR method, then positive rates of genes were assessed with capillary electrophoresis. Theresistance phenotypes were detected with text. Results Among the 72 strains of GAS, all isolates were susceptible to penicillin, levofloxacin, chloramphenicol, vancomycin and cefotaxime. The resistance rates of erythromycin, clindamycin and tetracycline were 100%, 100% and 94.44%, respectively. Two resistance genes, ermB and tetM, were detected (both 100%), while ermA, mefA and tetO were not. Only cMLS phenotype was found in all isolates (100%), while iMLS and M phenotype were not (0%). Conclusion The resistance pattern of GAS in 4 cities of Shandong Province in 2013 was multiple antibiotic resistance of erythromycin-clindamycin-tetracycline. The resistance phenotype was cMLS, and the genotypes were mainly co-carried ermB and tetM.

Key words: Resistance genes, Children, Drug resistance, Bacterial susceptibility testing, Streptococcus pyogenes, Shandong Province

CLC Number: 

  • R446.5
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