Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (8): 24-30.doi: 10.6040/j.issn.1671-7554.0.2023.0017

• 基础医学 • Previous Articles    

Effects and mechanism of Hanjingtang on the oxidative stress induced by cold stimulation in aortic vascular smooth muscle cells

LIU Yang1, CHEN Guihai2   

  1. 1. College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China;
    2. Basic Medical School, Guangxi University of Traditional Chinese Medicine, Nanning 530000, Guangxi, China
  • Published:2023-08-30

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Abstract: Objective To observe the effects and mechanism of Hanjingtang on the oxidative stress induced by cold stimulation in aortic vascular smooth muscle cells. Methods The experimental cells were divided into blank control group, model group, Hanjingtang(HJT)group and dimethyl fumarate(DMF)group. The DMF group was defined as the positive control. After vascular smooth muscle cells(A7r5)were cultured for 24 hours, they received cold stimulation at 4 ℃ for 4 hours. Cell viability was detected with CCK-8; expressions of superoxide dismutase(SOD)and malonaldehyde(MDA)were determined with immunochemistry; expression of active oxygen(ROS)was detected with DCFH-DA fluorescence; expressions of interleukin-1β(IL-1β)and nucleotide-binding oligomeric domain-like receptor protein 3(NLRP3)were assessed with Elisa; protein expressions of cellular nuclear factor E2-associated factor 2(Nrf 2), NAD(P)H quinone oxidoreductase 1(NQO1), nuclear factor κB(NF-κB), and human nuclear factor κB inhibitor protein α(IκBα)were determined with Western blotting; mRNA expressions of NQO1 and NF-κB were detected with quantitative real-time PCR. Results Compared with the blank control group, the model group had decreased cell viability and SOD(P<0.01); increased ROS, MDA, IL-1β and NLRP3 levels; increased protein expressions of NF-κB and IκBα, but decreased protein expressions of Nrf2 and NQO1(P<0.01); increased mRNA expression of NF-κB, but decreased mRNA expression of NQO1(P<0.01). Compared with the model group, the HJT group had increased cell viability and SOD(P<0.01); decreased ROS, IL-1β, NLRP3(P<0.01), and MDA(P<0.05)levels; increased protein expressions of Nrf2 and NQO1(P<0.01), but decreased protein expressions of NF-κB and IκBα(P<0.05, P<0.01); decreased mRNA expression of NF-κB(P<0.01), but increased mRNA expression of NQO1(P<0.05). Conclusion The mechanism of enhancing cold-stimulated aortic smooth muscle cell vitality, regulating cellular ROS expression, inhibiting inflammatory factor expression, and improving oxidative stress response may be related to Nrf2/NF-κB pathway.

Key words: Cold stimulation, Oxidative stress, Hanjingtang, Nuclear factor E2-associated factor 2, Nuclear factor κB

CLC Number: 

  • R259
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