Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (10): 49-56.doi: 10.6040/j.issn.1671-7554.0.2022.0360

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Distribution characteristics of nasopharyngeal microbiota of 312 healthy infants under 6 months of age detected by MALDI-TOF MS

GUAN Hengyun1, LIU Lanzheng1, JIANG Huiyu1, BAI Aiying1, LIU Hui1, ZHAO Xiaodong2, GAO Shang3, LI Na1, RUAN Shiman1   

  1. 1. Bacterial Disease Inspection Laboratory;
    2. Immunology Institute;
    3. Institute for Infectious Diseases Control, Jinan Center for Disease Control and Prevention, Jinan 250021, Shandong, China
  • Published:2022-09-30

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Abstract: Objective To explore the distribution characteristics of nasopharyngeal microflora in healthy infants under 6 months of age using matrix-assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)so as to provide sample bank information for nasopharyngeal microecology research. Methods Nasopharyngeal swabs were collected from 312 healthy infants during Nov. 2019 and April 2020 in Jinan. The pure strains were obtained after culturing and isolating. The bacterial strains were identified by MALDI-TOF MS to the species-level. The infants were divided into four age groups(≤ 2 months, 3 months group, 4 months group and 5 months group). SPSS 22.0 software was used for chi-square test or Fisher exact test between groups. Results A total of 741 bacterial strains were isolated, including 74 species of 25 genera of bacteria. The dominant genera were Staphylococcus(31.6%), Corynebacterium(30.8%), Streptococcus(18.9%), Moraxella(8.6%)and Dolosicoccus(2.0%). Some uncommon bacteria were also found, such as Abiotrophia defectiva, Rhizobium radiobacter, et al. The detection rate of Staphylococcus aureus was higher in male infants(40.5%)than in female infants(26.4%), while the detection rate of Corynebacterium propinquum was higher in female infants(42.4%)than in male infants(27.4%). In the four age groups, the detection rate of Corynebacterium pseudodiphtheriticum was increased successively(10.8%, 17.7%, 18.9% and 46.7%), as the same for Streptococcus pneumoniae(2.2%, 2.3%, 4.1% and 20.0%). Conclusion The diversity is mainly characterized by dominant bacteria in nasopharyngeal microflora for healthy infants under 6 months of age in Jinan, China. MALDI-TOF MS is a rapid and high-throughput technique for identifying common and uncommon bacterial species. The results can provide valuable reference for the study of nasopharyngeal microecology of children in Jinan.

Key words: Nasopharynx, Matrix assisted laser desorption ionization time of flight mass spectrometry, Micro-ecology, Infant

CLC Number: 

  • R183.3
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