Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (2): 1-6.doi: 10.6040/j.issn.1671-7554.0.2020.0934

    Next Articles

Effects of signal Crosstalk between glioma cells and vascular endothelial cells on the proliferation and invasion of glioma cells

GU Jinhai1, LU Ning2, GU Jiarong3, WEN Yujun1, QIANG Yuanyuan1, HE Zhenquan1, YANG Yong1, WANG Feng1, SUN Tao1, NIU Jianguo1   

  1. 1. Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Department of Neurosurgery, The First Peoples Hospital of Yinchuan, Yinchuan 750001, Ningxia, China;
    3. Department of Preventive Medicine, Medical School of Ningbo University, Ningbo 315211, Zhejiang, China
  • Published:2021-03-05

PDF (PC)

333

Abstract: Objective To explore the signal Crosstalk between glioma cells and human brain microvascular endothelial cells(HBMECs)and its effects on the proliferation and invasion of glioma cells. Methods HBMECs were divided into four groups: the control group was cultured with conventional endothelial cell medium(ECM); the vascular endothelial growth factor(VEGF)group was cultured with ECM treated with VEGF165(50 ng/mL); U87MG co-culture group was co-cultured with U87MG cells; U251 co-culture group was co-cultured with U251 cells. Then ElISA was performed to detect the effects of glioma cells and VEGF on the secretion of CXCL8. The glioma cells(U87MG/U251)were divided into four groups: the control group was cultured with conventional DMEM; U87MG/U251+HBMEC group was co-cultured with HBMECs, U87MG/U251+HBMEC+rhCXCL8 group was co-cultured with HBMECs in DMEM containing rhCXCL8(50 ng/mL, Akt pathway agonist), U87MG/U251+HBMEC+ LY29400 group were co-cultured with HBMECs in DMEM containing LY294002(1 μmol/L, Akt pathway inhibitor). Then Western blotting, CCK-8 and Transwell assays were performed to examine the effects of HBMECs and secretion of CXCL8 on the proliferation and invasion of glioma cells and expression of Akt. Results The secretion of CXCL8 in the VEGF group, U87MG co-culture group and U251 co-culture group were significantly increased compared with that in the control group(P<0.05), which indicated that glioma cells and VEGF could promote the secretion of CXCL8. Compared with the control group, the U87MG/U251+HBMEC group had elevated P-Akt, proliferation and invasion(P<0.05); exposure to CXCL8 further increased P-Akt, proliferation and invasion(P<0.01), whereas exposure to LY294002 suppressed P-Akt(P<0.01), which indicated that HBMECs could activate Akt signaling pathway by secreting CXCL8, and promote the proliferation and invasion of glioma cells. Conclusion There is a crosstalk of VEGF-CXCL8-Akt signal network between glioma cells and HBMECs, which might play an important role in the proliferation and invasion of glioma cells.

Key words: Glioma, Human brain microvascular endothelial cells, Crosstalk, Vascular endothelial growth factor, CXCL8, Akt

CLC Number: 

  • R730.264
[1] WANG Lin-Lin, SUN Mei-Li, SUN Yu-Ping, ZHANG Nan, LIU Chuan-Yong. Expressions of α-Tubulin and γ-Tubulin in human Glioma and their relation with Survivin [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 103-.
[2] HUANG Fang, KANG Rui, TUN Chun-Lin. Expressions of VEGF-C，nuclear factor-kappa B and  Survivin in nasopharyngeal carcinoma and their clinical implications [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 83-.
[3] SUN Qingjie, ZHANG Yisha, GUAN Shanghui, FENG Zhihui. Effects of valproic acid on the survival and tumor recurrence of 134 patients with glioma treated with radiotherapy [J]. Journal of Shandong University (Health Sciences), 2021, 59(8): 80-85.
[4] ZHANG Qian, QIN Mingming, HE Xuejia, CAI Qiujing, ZHANG Yamin, LI Qingsu, ZHU Weiwei. Effects of calcitriol on EMT induced by TGF-β1 in asthma [J]. Journal of Shandong University (Health Sciences), 2021, 59(7): 10-18.
[5] SONG Zhenzhen, SUN Xiaoling, LI Haiou, WANG Fang, ZHANG Ran, YU Dexin. Value of MRI radiomics of glioma and peritumoral edema in evaluating tumor recurrence [J]. Journal of Shandong University (Health Sciences), 2021, 59(11): 53-60.
[6] LI Wenqing, YE Lan, JIANG Yuhua. CDK7 inhibitor THZ1 increases the radiosensitivity of human glioma cell line U251 [J]. Journal of Shandong University (Health Sciences), 2021, 59(1): 8-13.
[7] Qiang WU,Zekun HE,Ju LIU,Xiaomeng CUI,Shuang SUN,Wei SHI. A research on multi-modal MRI analysis based on machine learning for brain glioma [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 81-87.
[8] Tao JIANG. The application of brain-like intelligence in the frontiers of brain science [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 10-13.
[9] Anjing CHEN,Xun ZHANG. The new strategies of targeting SUMOylation in the treatment of glioma [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 88-94.
[10] Gang LI,Hao XUE,Wei QIU,Rongrong ZHAO. Research advances in the formation of glioma immunosuppressive microenvironment [J]. Journal of Shandong University (Health Sciences), 2020, 1(8): 67-73.
[11] XU Jixi, CHEN Weijian. Diffuse midline glioma with H3 K27M mutation in the spinal cord: a case report [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 96-101.
[12] WANG Ying, ZHANG Yafei, WEN Yong. YAP gene overexpression promotes tongue squamous carcinoma growth through PI3K/AKT/mTOR signaling pathway [J]. Journal of Shandong University (Health Sciences), 2020, 58(2): 21-28.
[13] WANG Jian, LI Jian, WANG Yong, ZHU Yaofeng. Baicalein induces apoptosis of bladder cancer cells via inhibition of the PI3K/AKT/mTOR pathway [J]. Journal of Shandong University (Health Sciences), 2019, 57(9): 74-82.
[14] LI Hongyan, FANG Lei, ZHANG Hongying, SU Yuan, LI Ling. A clinical study of 5 cases of leucine-rich glioma inactivated 1 protein antibody associated limbic encephalitis [J]. Journal of Shandong University (Health Sciences), 2019, 57(5): 105-109.
[15] ZHAO Xueying, FAN Mingde, DONG Wenyan, QI Yingzhi, LU Xin. Expression and clinical significance of tripartite motif protein 26 in glioma tissues [J]. Journal of Shandong University (Health Sciences), 2019, 57(3): 63-68.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] WANG Huan-liang,SUN Bao-zhu,DU Hong-mei,ZHOU Chang-qing,ZHANG Li. Controlling the depth of propofol anesthesia using the bispectral indexand the systolic blood pressure[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(5): 471 -474 .
[2] HUANGQing,TIAN Hui,LI Lin,LIANG Fei,LIU Xi-xian. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(6): 556 -559 .
[3] ZHU Mei-jia,HAN Ju,WANG Xin-yi,LU Wei,WANG Ai-hua,GUAN Xin-hua,CAO Xia,CAO Bing-zhen. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(8): 834 -839 .
[4] SONG Hai-yan, WU Yu-ling, ZHANG Yan-ping. Protective effect of oyster extract on apoptotic cells in neural tube defect induced by hyperthermia[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(2): 113 -116 .
[5] SONG Yong-hong,MA Chun-hong,LV Hong-juan,ZHU Chuan-fu,NIE Xiang-min,WANG Mei,LIU Yan,ZHANG Ping. HLA allele polymorphism in northern Hans of China[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(6): 546 -553 .
[6] WANG Zhi-ang,DING Xua,SUN Peng/sup>,WANG Cheng-wei,HAO Xiao-guang,PAN Shun. Role of pre-operative cerebral angiography in cerebral artery stenosis stent angioplasty[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(2): 146 -148 .
[7] HOU Xiao-yang,BU Pei-li,ZHANG Yun,FENG Jin-bo,LIU Chun-xi,LI Chuan-bao,HAO Ming-xiu. Peroxisome proliferatorsactivated receptor α play a critical role in the inhibition of myocardial fibrosis induced by AngⅡ in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(7): 665 -668 .
[8] ZHANG Xiang-li,LIU Feng-ying. Correlation between serum TPA and sVCAM-1 and pathogenesis of pre-eclampsia[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(7): 705 -707 .
[9] YU Hui1,2, CHEN Shaohua1, ZHAO Jiajun2, GAO Ling3
. Effects of ethanol on glycogen content, GSK3β，
and PAMPK of L02 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(04): 75 -78 .
[10] . Association between polymorphism of the angiotensinconverting enzyme gene and the aldosterone synthase gene in pedigreed primary hypertension[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(7): 44 -48 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd