Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (2): 21-28.doi: 10.6040/j.issn.1671-7554.0.2019.1034

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YAP gene overexpression promotes tongue squamous carcinoma growth through PI3K/AKT/mTOR signaling pathway

WANG Ying, ZHANG Yafei, WEN Yong   

  1. Department of Implantology, School and Hospital of Stomatology, Shandong University &
    Shandong Key Laboratory of Oral Tissue regeneration &
    Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, Shandong, China
  • Online:2020-02-10 Published:2022-09-27

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Abstract: Objective To explore the molecular mechanism of YAP gene overexpression on the proliferation and apoptosis of tongue squamous carcinoma cells through the PI3K/AKT/mTOR signaling pathway. Methods CAL27 and SCC25 cell lines overexpressing YAP gene were constructed. The effects of YAP gene overexpression on cell proliferation, cell cycle and apoptosis were detected with CCK-8 and flow cytometry. Effects of YAP gene overexpression on the CDK4, CDK6, p21, BAX and PI3K/AKT/mTOR signaling pathways were analyzed with Western blotting. Results The mRNA expression of YAP in the overexpressing group was higher than that in the control group(t=-19.29, P<0.001; t=-5.44, P<0.001), and the protein expression was also higher(t=-31.28, P<0.001; t=-42.84, P<0.001). In the overexpressing group, the proportion of cells in G1 phase decreased, while the proportion of cells in S phase 山 东 大 学 学 报 (医 学 版)58卷2期 -王莹,等.过表达YAP基因通过PI3K/AKT/mTOR信号通路促进舌鳞癌增殖 \=-increased; the numbers of both early apoptosis and late apoptosis cells reduced; the protein expressions of CDK4, CDK6, BCL-2, PARP increased, while the protein expressions of p21, BAX and caspase3 decreased. YAP gene overexpression increased the expressions of AKT(tCAL27=-30.60, P<0.001; tSCC25=-16.77, P<0.001), p-AKT(tCAL27=-10.23, P<0.001; tSCC25=-10.67, P<0.001), mTOR(tCAL27=-13.93, P<0.001; tSCC25=-9.57, P<0.001), and p-mTOR(tCAL27=-17.17, P<0.001; tSCC25=-8.51, P<0.001). Conclusion YAP gene overexpression promotes proliferation of CAL27 and SCC25 cells and inhibits apoptosis through the PI3K/AKT/mTOR signaling pathway.

Key words: YAP gene overexpression, Tongue squamous cell carcinoma, Proliferation, Apoptosis, PI3K/AKT/mTOR signaling pathway

CLC Number: 

  • R739.86
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