Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (12): 47-54.doi: 10.6040/j.issn.1671-7554.0.2018.049

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Expression of karyopherin α2 and its effect on the biological behaviors of glioma

LI Jie, HE Dong, ZHANG Rui, YANG Fan, FENG Shaobin, YANG Yihang, XIN Tao   

  1. Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the clinical significance and biological effects of karyopherin α2(KPNA2)on glioma. Methods A total of 90 specimens of cerebral astrocytoma were collected, including WHO grades Ⅱ, Ⅲ and Ⅳ(30 cases for each grade), and 15 normal peritumoral tissues served as controls. The expression of KPNA2 was detected with qRT-PCR and immunohistochemistry. After that, the lentiviruses expressing small hairpin RNAs(shRNAs)of KPNA2 were introduced into the U87 and U251 glioma cells. The effects of KPNA2-knockdown on the proliferation of glioma cells were detected with CCK8 and EdU, and the effects on the cell migration and invasion were assessed with Transwell assay. Results KPAN2 was overexpressed in glioma tissues, and high level of KPNA2 was positively correlated with the WHO classification of glioma(rs=0.559, P<0.001)and negatively correlated with the prognosis of patients(overall survival: χ2=21.39, P<0.001; relapse-free survival: χ2=8.057, P=0.004). In addition, KPNA2-knockdown significantly weakened the proliferation, migration and invasion of glioma cell lines U87 and U251. Conclusion KPNA2 plays an important role in the tumorigenesis and development of glioma and may serve as a novel biomarker or therapeutic target for clinical diagnosis and treatment.

Key words: Karyopherin α2, Glioma, Clinicopathologic features, Prognosis, Cell proliferation

CLC Number: 

  • R574
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