JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (4): 25-31.doi: 10.6040/j.issn.1671-7554.0.2015.1079

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Neuroprotective effect of dl-3n-butylphthalide on focal cerebral ischemia reperfusion injury in rats

ZHAO Xiuqin1, DENG Chunying1, LI Shiying1, ZHANG Jinxia1, HE Yonggui1, YU Hong2, LIU Bin1   

  1. 1. Department of Neurology;
    2.Department of pharmacology, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan 063000, Heibei, China
  • Received:2015-11-06 Online:2016-04-10 Published:2016-04-10

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Abstract: Objective To explore the neuroprotective effect of dl-3n-butylphthalide(NBP)on focal cerebral ischemia reperfusion injury in rats and to investigate the possible mechanism. Methods A total of 75 male Sprague-Dawley(SD)rats were randomly divided into Sham operation group(Sham group), ischemia/reperfusion injury group(IR group), high-dose NBP post-treatment group(high-dose group), medium-dose NBP post-treatment group(medium-dose group)and low-dose NBP post-treatment group(low-dose group), with 15 rats in each group. Focal cerebral ischemia reperfusion model was established with middle cerebral artery occlusion(MCAO)by modified cerebral artery ligation. At 24 h after 2 hours reperfusion, neurological deficits and the infarct volume were measured. TdT-mediated dUTP nick-end labeling(TUNEL)was used to observe the expression of neuronal apoptosis. Immunohistochemistry was used to observe silent mating type information regulation 2 homolog 1(SIRT1)and peroxisome proliferator-activated receptor-γ coactivator-1α(PGC-1α)positive cells. Quantitative Real-time PCR was used to observe the expression of SIRT1 and PGC-1α mRNA. Results The rats in Sham group showed no obvious neurological deficit symptoms and no 山 东 大 学 学 报 (医 学 版)54卷4期 -赵秀芹,等.丁苯酞注射液对大鼠局灶性脑缺血再灌注损伤的神经保护作用 \=-infarct size. Compared with Sham group, the number of apoptotic cells increased in IR group and three dosage levels of NBP post-treatment group(P<0.05), so were the number of SIRT1 and PGC-1α positive cells and the expression of SIRT1 and PGC-1α mRNA(P<0.05). Compared with IR group, the neurological deficit scores decreased in each three dosage level of NBP post-treatment group(P<0.05), so were the infarct size and the number of apoptotic cells(P<0.05), while the number of SIRT1 and PGC-1α positive cells and the expression of SIRT1 and PGC-1α mRNA in NBP post-treatment group were increased(P<0.05). Compared with medium- or low- dose group, the high-dose group had lowest neurological deficit scores, infarct size and the number of apoptotic cells, but highest number of SIRT1 and PGC-1α positive cells and the expression of SIRT1 and PGC-1α mRNA, with significant difference(P<0.05). Conclusion NBP injection can relieve the brain damage induced by focal cerebral ischemia reperfusion in rats, which potentially related to the up-regulation of SIRT1 and PGC-1α expression.

Key words: Butylphthalide, Focal cerebral ischemia reperfuson, Silent mating type information regulation 2 homolog 1, Peroxisome proliferator-activated receptor-γ coactivator-1α, Neuroprotective effect

CLC Number: 

  • R743
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